P96989 (OMPB_RICTY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Outer membrane protein B

Alternative name(s):
168 kDa surface-layer protein
Cell surface antigen 5
Short name=Sca5
Surface protein antigen
rOmp B
Short name=rOmpB

Cleaved into the following 2 chains:

1. 120 kDa surface-exposed protein
   Alternative name(s):
   120 kDa outer membrane protein OmpB
   Surface protein antigen
   p120
2. 32 kDa beta peptide

Gene names

Name:	ompB
Synonyms:	slp
Ordered Locus Names:	RT0699

Organism

Rickettsia typhi (strain ATCC VR-144 / Wilmington) [Complete proteome] [HAMAP]

Taxonomic identifier

257363 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group › Rickettsia typhi

Protein attributes

Sequence length	1645 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The 120 kDa surface-exposed protein is a major structural protein which may play a role as a rickettsial virulence factor and/or immunogen during infection. The 32 kDa beta peptide may serve as a membrane anchor.
Subcellular location	Outer membrane protein B: Periplasm By similarity. 120 kDa surface-exposed protein: Secreted. Cell surface. Note: Surface exposed. This bacterium is covered by a S-layer with hexagonal symmetry. 32 kDa beta peptide: Cell outer membrane; Multi-pass membrane protein Potential. Note: The cleaved C-terminal fragment (autotransporter domain) is localized in the outer membrane.
Sequence similarities	Belongs to the rickettsiae OmpA/OmpB family. Contains 1 autotransporter (TC 1.B.12) domain. [View classification]

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell membrane Cell outer membrane Membrane Periplasm Secreted
Domain	Transmembrane Transmembrane beta strand
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1645	1645	Outer membrane protein B		PRO_0000387583
	Chain	1 – 1328	1328	120 kDa surface-exposed protein		PRO_0000032660
	Propeptide	1329 – 1352	24	Potential		PRO_0000032661
	Chain	1353 – 1645	293	32 kDa beta peptide		PRO_0000032662
Regions
	Domain	1357 – 1645	289	Autotransporter
Experimental info
	Sequence conflict	657	1	H → N AA sequence Ref.3
	Sequence conflict	842	1	V → I AA sequence Ref.3
	Sequence conflict	1071	1	G → A AA sequence Ref.3
	Sequence conflict	1306	1	G → S AA sequence Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P96989 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0CB5641C7EB185EE	FASTA	1,645	169,698
	10 20 30 40 50 60 MAQKPNFLKK IISAGLVTAS TATIVAGFSG VAMGAVMQYN RTTNAAATTV DGAGFDQTGA 70 80 90 100 110 120 GVNLPVATNS VITANSNNAI TFNTPNGNLN SLFLDTANTL AVTINENTTL GFVTNVTKQG 130 140 150 160 170 180 NFFNFTIGAG KSLTITGHGI TAQQAATTKS AQNVVSKVNA GAAINDNDLS GVGSIDFTAA 190 200 210 220 230 240 PSVLEFNLIN PTTQEAPLTL GDNAKIVNGA NGILNITNGF VKVSDKTFAG IKTINIGDNQ 250 260 270 280 290 300 GLMFNTTPDA ANALNLQGGG NTINFNGRDG TGKLVLVSKN GNATEFNVTG SLGGNLKGVI 310 320 330 340 350 360 EFDTTAAAGK LIANGGAANA VIGTDNGAGR AAGFIVSVDN GNAATISGQV YAKDIVIQSA 370 380 390 400 410 420 NAGGQVTFEH LVDVGLGGKT NFKTADSKVI ITENASFGST DFGNLAVQIV VPNNKILTGN 430 440 450 460 470 480 FIGDAKNNGN TAGVITFNAN GTLVSGNTDP NIVVTNIKAI EVEGAGIVQL SGIHGAELRL 490 500 510 520 530 540 GNAGSIFKLA DGTVINGPVN QNPLVNNNAL AAGSIQLDGS AIITGDIGNG AVNAALQDIT 550 560 570 580 590 600 LANDASKILT LSGANIIGAN AGGAIHFQAN GGTIQLTSTQ NNILVDFDLD VTTDQTGVVD 610 620 630 640 650 660 ASSLTNNQTL TINGSIGTIG ANTKTLGRFN VGSSKTILNA GDVAINELVM ENDGSVHLTH 670 680 690 700 710 720 NTYLITKTIN AANQGKIIVA ADPINTDTAL ADGTNLGSAE SPLSNIHFAT KAANGDSILH 730 740 750 760 770 780 IGKGVNLYAN NITTTDANVG SLHFRSGGTS IVSGTVGGQQ GLKLNNLILD NGTTVKFLGD 790 800 810 820 830 840 ITFNGGTKIE GKSILQISSN YITDHIESAD NTGTLEFVNT DPITVTLNKQ GAYFGVLKQV 850 860 870 880 890 900 MVSGPGNIAF NEIGNGVAHA IAVDSISFEN ASLGASLFLL SGTPLDVLTI KSTVGNGTVD 910 920 930 940 950 960 NFNAPILVVS GIDSMINNGQ VIGDQKNIIA LSLGSDNSIT VNSNTLYAGI RTTKTNQGTV 970 980 990 1000 1010 1020 TLSGGIPNNP GTIYGLGLEN GDPKLKQVTF TTDYNNLGSI IATNVTINDD VTLTTGGIAG 1030 1040 1050 1060 1070 1080 TDFDGKITLG SINGNANVKF VDRTFSHPTS MIVSTKANQG TVTYLGNALV GNIGSSDIPV 1090 1100 1110 1120 1130 1140 ASVRFTGNDS GVGLQGNIHS QNIDFGTYNL TILNSDVILG GGTTAINGEI DLLTNNLIFA 1150 1160 1170 1180 1190 1200 NGTSTWGNNT SLSTTLNVSN GNVGQIVIAE GAQVNATTTG TTTIKIQDNA NANFSGTQTY 1210 1220 1230 1240 1250 1260 TLIQGGARFN GTLGAPNFDV TGNNIFVKYE LIRDANQDYV LTRTNDVLNV VTTAVGNSAI 1270 1280 1290 1300 1310 1320 ANAPGVHQNI AICLESTDTA AYNNMLLAKD SSDVATFIGA IATDTGAAVA TVNLNDTQKT 1330 1340 1350 1360 1370 1380 QDLLGNRLGA LRYLSNSETA DVGGSETGAV SSGDEAIDQV SYGVWAKPFY NIAEQDKKGG 1390 1400 1410 1420 1430 1440 LAGYKAKTAG VVVGLDTLAN DNLMIGAAIG ITKTDIKHQD YKKGDKTDIK GLSFSLYGAQ 1450 1460 1470 1480 1490 1500 QLVKNFFAQG SAIFTLNKVK SKSQRYFFDA NGKMNKQIAA GNYDNITFGG NLMFGYDYNA 1510 1520 1530 1540 1550 1560 LQGVLVTPMA GLSYLKSSNE NYKETGTTVA NKRIHSKFSD RIDLIVGAKV TGSAMNINDI 1570 1580 1590 1600 1610 1620 VIYPEIHSFV VHKVNGKLSK AQSMLDGQTA PFISQPDRTA KTSYNIGLSA NIRSDAKMEY 1630 1640 GIGYDFNAAS KYTAHQGTLK VRINF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of the gene encoding the crystalline surface layer protein of Rickettsia typhi." Hahn M.-J., Kim K.-K., Kim I., Chang W.-H. Gene 133:129-133(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.
[2]	"Complete genome sequence of Rickettsia typhi and comparison with sequences of other Rickettsiae." McLeod M.P., Qin X., Karpathy S.E., Gioia J., Highlander S.K., Fox G.E., McNeill T.Z., Jiang H., Muzny D., Jacob L.S., Hawes A.C., Sodergren E., Gill R., Hume J., Morgan M., Fan G., Amin A.G., Gibbs R.A. , Hong C., Yu X.-J., Walker D.H., Weinstock G.M. J. Bacteriol. 186:5842-5855(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC VR-144 / Wilmington.
[3]	"Mapping of monoclonal antibody binding sites on CNBr fragments of the S-layer protein antigens of Rickettsia typhi and Rickettsia prowazekii." Ching W.M., Carl M., Dasch G.A. Mol. Immunol. 29:95-105(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Strain: ATCC VR-144 / Wilmington.
[4]	"Evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing." Hackstadt T., Messer R., Cieplak W. Jr., Peacock M.G. Infect. Immun. 60:159-165(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1353-1371, IDENTIFICATION OF CLEAVAGE SITE. Strain: ATCC VR-144 / Wilmington.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L04661 Unassigned DNA. Translation: AAB48987.1. AE017197 Genomic DNA. Translation: AAU04158.1.
PIR	JN0896.
RefSeq	YP_067640.1. NC_006142.1.
3D structure databases
ProteinModelPortal	P96989.
ModBase	Search...
Protein-protein interaction databases
STRING	257363.RT0699.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAU04158; AAU04158; RT0699.
GeneID	2959181.
KEGG	rty:RT0699.
PATRIC	17910646. VBIRicTyp34752_0718.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG12793.
HOGENOM	HOG000010784.
OMA	TINAANQ.
ProtClustDB	CLSK871043.
Enzyme and pathway databases
BioCyc	RTYP257363:GJEQ-733-MONOMER.
Family and domain databases
InterPro	IPR005546. Auto_transptbeta. IPR006315. OM_autotransptr_brl. IPR022095. rOmpB. [Graphical view]
Pfam	PF03797. Autotransporter. 1 hit. PF12334. rOmpB. 1 hit. [Graphical view]
SMART	SM00869. Autotransporter. 1 hit. [Graphical view]
SUPFAM	SSF103515. Auto_transptbeta. 1 hit.
TIGRFAMs	TIGR01414. autotrans_barl. 1 hit.
PROSITE	PS51208. AUTOTRANSPORTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OMPB_RICTY

Accession

Primary (citable) accession number: P96989

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program