P96985 (TPIS3_RHIEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Triosephosphate isomerase
Short name=TIM
EC=5.3.1.1

Alternative name(s):
Triose-phosphate isomerase

Gene names

Name:	tpiA
Synonyms:	tpi

Organism

Rhizobium etli (strain CFN 42 / ATCC 51251)

Taxonomic identifier

347834 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	226 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147_B
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147_B Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147_B
Subunit structure	Homodimer By similarity. HAMAP MF_00147_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00147_B.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: UniProtKB-KW glycolysis Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›226	›226	Triosephosphate isomerase HAMAP MF_00147_B		PRO_0000090273
Sites
	Active site	91	1	Electrophile By similarity
	Active site	163	1	Proton acceptor By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	226	1

Sequences

	Sequence		Length	Mass (Da)
	P96985 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 8F23101844618DDB	FASTA	226	24,292
	10 20 30 40 50 60 MNPMQADAKQ LLQEFKQLLQ ENEITEEKCN IGLAPVTLAL TSTQAELANA ARSVFTVAQD 70 80 90 100 110 120 VSRFGNMGAY TGEVSAELLK DSQIEYVLIG HSERREYFAE SAAILNAKAQ NALNAGLKVI 130 140 150 160 170 180 YCVGESLEQR ESGQAEVVVL QQICDLASVV TAEQWPHIVI AYEPIWAIGT GKTASPEDAQ 190 200 210 220 TMHAKIREGL TQITSHGANM AILYGGSVKA ENAVELAACP DINGAL

References

[1]	"Evidence that eukaryotic triosephosphate isomerase is of alpha-proteobacterial origin." Keeling P.J., Doolittle W.F. Proc. Natl. Acad. Sci. U.S.A. 94:1270-1275(1997) [PubMed: 9037042] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73964 Genomic DNA. Translation: AAB48823.1.
3D structure databases
ProteinModelPortal	P96985.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG0149.
Family and domain databases
HAMAP	MF_00147_B. TIM_B. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. IPR020861. Triosephosphate_isomerase_AS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
SUPFAM	SSF51351. Triophos_ismrse. 1 hit.
TIGRFAMs	TIGR00419. Tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS3_RHIEC

Accession

Primary (citable) accession number: P96985

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program