P96965 (P96965_PSEFL) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase EMBL BAA12150.1

Gene names

Name:

cumD EMBL BAA12150.1

Organism

Pseudomonas fluorescens EMBL BAA12150.1

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Hydrolase EMBL BAA12150.1
Technical term	3D-structure PDB 1UKA PDB 1UKB PDB 1UK6 PDB 1UK7 PDB 1UK9 PDB 2D0D PDB 1IUP PDB 1UK8 PDB 1IUO PDB 1IUN
Gene Ontology (GO)
Molecular function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	P96965 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 2FA69148F390DED9	FASTA	282	31,490
	10 20 30 40 50 60 MANLEIGKSI LAAGVLTNYH DVGEGQPVIL IHGSGPGVSA YANWRLTIPA LSKFYRVIAP 70 80 90 100 110 120 DMVGFGFTDR PENYNYSKDS WVDHIIGIMD ALEIEKAHIV GNSFGGGLAI ATALRYSERV 130 140 150 160 170 180 DRMVLMGAAG TRFDVTEGLN AVWGYTPSIE NMRNLLDIFA YDRSLVTDEL ARLRYEASIQ 190 200 210 220 230 240 PGFQESFSSM FPEPRQRWID ALASSDEDIK TLPNETLIIH GREDQVVPLS SSLRLGELID 250 260 270 280 RAQLHVFGRC GHWTQIEQTD RFNRLVVEFF NEANTPKLVG RP

References

[1]	"Analysis of cumene (isopropylbenzene) degradation genes from Pseudomonas fluorescens IP01." Habe H., Kasuga K., Nojiri H., Yamane H., Omori T. Appl. Environ. Microbiol. 62:4471-4477(1996) [PubMed: 8953719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: IP01 EMBL BAA12150.1.
[2]	"Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products." Fushinobu S., Saku T., Hidaka M., Jun S.Y., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T. Protein Sci. 11:2184-2195(2002) [PubMed: 12192074] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS).
[3]	"A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products." Fushinobu S., Jun S.Y., Hidaka M., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T. Biosci. Biotechnol. Biochem. 69:491-498(2005) [PubMed: 15784976] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS).
[4]	"Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01." Jun S.Y., Fushinobu S., Nojiri H., Omori T., Shoun H., Wakagi T. Biochim. Biophys. Acta 1764:1159-1166(2006) [PubMed: 16844437] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D83955 Genomic DNA. Translation: BAA12150.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IUN	X-ray	2.80	A/B	1-282	[»]
1IUO	X-ray	2.00	A	1-282	[»]
1IUP	X-ray	1.60	A	1-282	[»]
1UK6	X-ray	1.95	A	1-282	[»]
1UK7	X-ray	1.70	A	1-282	[»]
1UK8	X-ray	1.60	A	1-282	[»]
1UK9	X-ray	1.80	A	1-282	[»]
1UKA	X-ray	1.70	A	1-282	[»]
1UKB	X-ray	1.80	A	1-282	[»]
2D0D	X-ray	1.65	A	1-282	[»]

ProteinModelPortal

P96965.

SMR

P96965. Positions 2-277.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PRINTS

PR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.

ProtoNet

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Entry information

Entry name

P96965_PSEFL

Accession

Primary (citable) accession number: P96965

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1997
Last sequence update:	May 1, 1997
Last modified:	January 11, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)