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P96965

- P96965_PSEFL

UniProt

P96965 - P96965_PSEFL

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Protein
Submitted name:

2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase

Gene

cumD

Organism
Pseudomonas fluorescens
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei103 – 1031Important for catalytic activityImported
Sitei224 – 2241Important for catalytic activityImported
Sitei252 – 2521Important for catalytic activityImported

GO - Molecular functioni

  1. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseImported

Names & Taxonomyi

Protein namesi
Submitted name:
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolaseImported
Gene namesi
Name:cumDImported
OrganismiPseudomonas fluorescensImported
Taxonomic identifieri294 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IUNX-ray2.80A/B1-282[»]
1IUOX-ray2.00A1-282[»]
1IUPX-ray1.60A1-282[»]
1UK6X-ray1.95A1-282[»]
1UK7X-ray1.70A1-282[»]
1UK8X-ray1.60A1-282[»]
1UK9X-ray1.80A1-282[»]
1UKAX-ray1.70A1-282[»]
1UKBX-ray1.80A1-282[»]
2D0DX-ray1.65A1-282[»]
ProteinModelPortaliP96965.
SMRiP96965. Positions 2-277.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96965.

Family & Domainsi

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

P96965-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANLEIGKSI LAAGVLTNYH DVGEGQPVIL IHGSGPGVSA YANWRLTIPA
60 70 80 90 100
LSKFYRVIAP DMVGFGFTDR PENYNYSKDS WVDHIIGIMD ALEIEKAHIV
110 120 130 140 150
GNSFGGGLAI ATALRYSERV DRMVLMGAAG TRFDVTEGLN AVWGYTPSIE
160 170 180 190 200
NMRNLLDIFA YDRSLVTDEL ARLRYEASIQ PGFQESFSSM FPEPRQRWID
210 220 230 240 250
ALASSDEDIK TLPNETLIIH GREDQVVPLS SSLRLGELID RAQLHVFGRC
260 270 280
GHWTQIEQTD RFNRLVVEFF NEANTPKLVG RP
Length:282
Mass (Da):31,490
Last modified:May 1, 1997 - v1
Checksum:i2FA69148F390DED9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83955 Genomic DNA. Translation: BAA12150.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83955 Genomic DNA. Translation: BAA12150.1 .

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IUN X-ray 2.80 A/B 1-282 [» ]
1IUO X-ray 2.00 A 1-282 [» ]
1IUP X-ray 1.60 A 1-282 [» ]
1UK6 X-ray 1.95 A 1-282 [» ]
1UK7 X-ray 1.70 A 1-282 [» ]
1UK8 X-ray 1.60 A 1-282 [» ]
1UK9 X-ray 1.80 A 1-282 [» ]
1UKA X-ray 1.70 A 1-282 [» ]
1UKB X-ray 1.80 A 1-282 [» ]
2D0D X-ray 1.65 A 1-282 [» ]
ProteinModelPortali P96965.
SMRi P96965. Positions 2-277.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P96965.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view ]
Pfami PF00561. Abhydrolase_1. 1 hit.
[Graphical view ]
PRINTSi PR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.
SUPFAMi SSF53474. SSF53474. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Analysis of cumene (isopropylbenzene) degradation genes from Pseudomonas fluorescens IP01."
    Habe H., Kasuga K., Nojiri H., Yamane H., Omori T.
    Appl. Environ. Microbiol. 62:4471-4477(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: IP01Imported.
  2. "Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products."
    Fushinobu S., Saku T., Hidaka M., Jun S.Y., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T.
    Protein Sci. 11:2184-2195(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS).
  3. "A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products."
    Fushinobu S., Jun S.Y., Hidaka M., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T.
    Biosci. Biotechnol. Biochem. 69:491-498(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS), ACTIVE SITE.
  4. "Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01."
    Jun S.Y., Fushinobu S., Nojiri H., Omori T., Shoun H., Wakagi T.
    Biochim. Biophys. Acta 1764:1159-1166(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Entry informationi

Entry nameiP96965_PSEFL
AccessioniPrimary (citable) accession number: P96965
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1997
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3