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P96965

- P96965_PSEFL

UniProt

P96965 - P96965_PSEFL

Protein
Submitted name:

2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase

Gene

cumD

Organism
Pseudomonas fluorescens
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341Acetate 1
    Sitei103 – 1031Important for catalytic activityImported
    Binding sitei215 – 2151Phosphate 2
    Sitei224 – 2241Important for catalytic activityImported
    Binding sitei252 – 2521Acetate 1
    Sitei252 – 2521Important for catalytic activityImported

    GO - Molecular functioni

    1. hydrolase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    HydrolaseImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolaseImported
    Gene namesi
    Name:cumDImported
    OrganismiPseudomonas fluorescensImported
    Taxonomic identifieri294 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IUNX-ray2.80A/B1-282[»]
    1IUOX-ray2.00A1-282[»]
    1IUPX-ray1.60A1-282[»]
    1UK6X-ray1.95A1-282[»]
    1UK7X-ray1.70A1-282[»]
    1UK8X-ray1.60A1-282[»]
    1UK9X-ray1.80A1-282[»]
    1UKAX-ray1.70A1-282[»]
    1UKBX-ray1.80A1-282[»]
    2D0DX-ray1.65A1-282[»]
    ProteinModelPortaliP96965.
    SMRiP96965. Positions 2-277.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP96965.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni121 – 1222Phosphate 1 binding
    Regioni258 – 2614Phosphate 2 binding

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PRINTSiPR00111. ABHYDROLASE.
    PR00412. EPOXHYDRLASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P96965-1 [UniParc]FASTAAdd to Basket

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    MANLEIGKSI LAAGVLTNYH DVGEGQPVIL IHGSGPGVSA YANWRLTIPA    50
    LSKFYRVIAP DMVGFGFTDR PENYNYSKDS WVDHIIGIMD ALEIEKAHIV 100
    GNSFGGGLAI ATALRYSERV DRMVLMGAAG TRFDVTEGLN AVWGYTPSIE 150
    NMRNLLDIFA YDRSLVTDEL ARLRYEASIQ PGFQESFSSM FPEPRQRWID 200
    ALASSDEDIK TLPNETLIIH GREDQVVPLS SSLRLGELID RAQLHVFGRC 250
    GHWTQIEQTD RFNRLVVEFF NEANTPKLVG RP 282
    Length:282
    Mass (Da):31,490
    Last modified:May 1, 1997 - v1
    Checksum:i2FA69148F390DED9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83955 Genomic DNA. Translation: BAA12150.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D83955 Genomic DNA. Translation: BAA12150.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IUN X-ray 2.80 A/B 1-282 [» ]
    1IUO X-ray 2.00 A 1-282 [» ]
    1IUP X-ray 1.60 A 1-282 [» ]
    1UK6 X-ray 1.95 A 1-282 [» ]
    1UK7 X-ray 1.70 A 1-282 [» ]
    1UK8 X-ray 1.60 A 1-282 [» ]
    1UK9 X-ray 1.80 A 1-282 [» ]
    1UKA X-ray 1.70 A 1-282 [» ]
    1UKB X-ray 1.80 A 1-282 [» ]
    2D0D X-ray 1.65 A 1-282 [» ]
    ProteinModelPortali P96965.
    SMRi P96965. Positions 2-277.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P96965.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000639. Epox_hydrolase-like.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00111. ABHYDROLASE.
    PR00412. EPOXHYDRLASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of cumene (isopropylbenzene) degradation genes from Pseudomonas fluorescens IP01."
      Habe H., Kasuga K., Nojiri H., Yamane H., Omori T.
      Appl. Environ. Microbiol. 62:4471-4477(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: IP01Imported.
    2. "Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products."
      Fushinobu S., Saku T., Hidaka M., Jun S.Y., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T.
      Protein Sci. 11:2184-2195(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS).
    3. "A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products."
      Fushinobu S., Jun S.Y., Hidaka M., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T.
      Biosci. Biotechnol. Biochem. 69:491-498(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS), ACTIVE SITE.
    4. "Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01."
      Jun S.Y., Fushinobu S., Nojiri H., Omori T., Shoun H., Wakagi T.
      Biochim. Biophys. Acta 1764:1159-1166(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

    Entry informationi

    Entry nameiP96965_PSEFL
    AccessioniPrimary (citable) accession number: P96965
    Entry historyi
    Integrated into UniProtKB/TrEMBL: May 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3