2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase

Preferred order of sections

Names and origin

Protein names

Submitted name:
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase EMBL BAA12150.1

Gene names

Name:

cumD EMBL BAA12150.1

Organism

Pseudomonas fluorescens EMBL BAA12150.1

Taxonomic identifier

294 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Hydrolase EMBL BAA12150.1
Technical term	3D-structure PDB 1UKA PDB 1UKB PDB 1UK6 PDB 1UK7 PDB 1UK9 PDB 2D0D PDB 1IUP PDB 1UK8 PDB 1IUO PDB 1IUN
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Site

103

1

Important for catalytic activity PDB 1UK7

Site

224

1

Important for catalytic activity PDB 1UK7

Site

252

1

Important for catalytic activity PDB 1UK7

Sequences

Sequence

Length

Mass (Da)

Tools

P96965 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 2FA69148F390DED9
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FASTA

282

31,490

        10         20         30         40         50         60 
MANLEIGKSI LAAGVLTNYH DVGEGQPVIL IHGSGPGVSA YANWRLTIPA LSKFYRVIAP 

        70         80         90        100        110        120 
DMVGFGFTDR PENYNYSKDS WVDHIIGIMD ALEIEKAHIV GNSFGGGLAI ATALRYSERV 

       130        140        150        160        170        180 
DRMVLMGAAG TRFDVTEGLN AVWGYTPSIE NMRNLLDIFA YDRSLVTDEL ARLRYEASIQ 

       190        200        210        220        230        240 
PGFQESFSSM FPEPRQRWID ALASSDEDIK TLPNETLIIH GREDQVVPLS SSLRLGELID 

       250        260        270        280 
RAQLHVFGRC GHWTQIEQTD RFNRLVVEFF NEANTPKLVG RP

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References

[1]	"Analysis of cumene (isopropylbenzene) degradation genes from Pseudomonas fluorescens IP01." Habe H., Kasuga K., Nojiri H., Yamane H., Omori T. Appl. Environ. Microbiol. 62:4471-4477(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: IP01 EMBL BAA12150.1.
[2]	"Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products." Fushinobu S., Saku T., Hidaka M., Jun S.Y., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T. Protein Sci. 11:2184-2195(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS).
[3]	"A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products." Fushinobu S., Jun S.Y., Hidaka M., Nojiri H., Yamane H., Shoun H., Omori T., Wakagi T. Biosci. Biotechnol. Biochem. 69:491-498(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS), ACTIVE SITE.
[4]	"Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01." Jun S.Y., Fushinobu S., Nojiri H., Omori T., Shoun H., Wakagi T. Biochim. Biophys. Acta 1764:1159-1166(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D83955 Genomic DNA. Translation: BAA12150.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IUN	X-ray	2.80	A/B	1-282	[»]
1IUO	X-ray	2.00	A	1-282	[»]
1IUP	X-ray	1.60	A	1-282	[»]
1UK6	X-ray	1.95	A	1-282	[»]
1UK7	X-ray	1.70	A	1-282	[»]
1UK8	X-ray	1.60	A	1-282	[»]
1UK9	X-ray	1.80	A	1-282	[»]
1UKA	X-ray	1.70	A	1-282	[»]
1UKB	X-ray	1.80	A	1-282	[»]
2D0D	X-ray	1.65	A	1-282	[»]

ProteinModelPortal

P96965.

SMR

P96965. Positions 2-277.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PRINTS

PR00111. ABHYDROLASE.
PR00412. EPOXHYDRLASE.

ProtoNet

Search...

Other

EvolutionaryTrace

P96965.

Entry information

Entry name

P96965_PSEFL

Accession

Primary (citable) accession number: P96965

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1997
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)