P96910 (GAL1_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactokinase
EC=2.7.1.6

Alternative name(s):
Galactose kinase

Gene names

Name:	galK
Ordered Locus Names:	Rv0620, MT0648
ORF Names:	MTCY19H5.01c, MTCY20H10.01

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP MF_00246
Pathway	Carbohydrate metabolism; galactose metabolism. HAMAP MF_00246
Subcellular location	Cytoplasm Potential HAMAP MF_00246.
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_00246
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW galactokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Galactokinase HAMAP MF_00246		PRO_0000184619
Regions
	Nucleotide binding	101 – 111	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P96910 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: 3424D08EF05C4AE9	FASTA	363	37,572
	10 20 30 40 50 60 MTVSYGAPGR VNLIGEHTDY NLGFALPIAL PRRTVVTFTP EHTGAITARS DRADGSARIP 70 80 90 100 110 120 LDTTPGQVTG WAAYAAGAIW ALRGAGHPVP GGAMSITSDV EIGSGLSSSA ALIGAVLGAV 130 140 150 160 170 180 GAATGTRIDR LERARLAQRA ENDYVGAPTG LLDHLAALFG APKTALLIDF RDITVRPVAF 190 200 210 220 230 240 DPDACDVVLL LMDSRARHCH AGGEYALRRA SCERAAADLG VSSLRAVQDR GLAALGAIAD 250 260 270 280 290 300 PIDARRARHV LTENQRVLDF AAALADSDFT AAGQLLTASH ESMREDFAIT TERIDLIAES 310 320 330 340 350 360 AVRAGALGAR MTGGGFGGAV IALVPADRAR DVADTVRRAA VTAGYDEPAV SRTYAAPGAA ECR

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842574 Genomic DNA. Translation: CAB09965.1. AE000516 Genomic DNA. Translation: AAK44872.1.
PIR	G70911.
RefSeq	NP_215134.1. NC_000962.2. NP_335058.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P96910.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003658; EBMYCP00000003658; EBMYCG00000003656. EBMYCT00000069775; EBMYCP00000067834; EBMYCG00000069770.
GeneID	887936. 925049.
GenomeReviews	Gene locus MT0648 in contig AE000516_GR. Gene locus Rv0620 in contig AL123456_GR.
KEGG	mtc:MT0648. mtu:Rv0620.
PATRIC	18123139. VBIMycTub22151_0702.
TIGR	MT0648.
Organism-specific databases
TubercuList	Rv0620.
Phylogenomic databases
GeneTree	EBGT00050000016589.
HOGENOM	HBG725121.
OMA	YNDGFVM.
PhylomeDB	P96910.
ProtClustDB	PRK00555.
Family and domain databases
HAMAP	MF_00246. Galactokinase. [Tree]
InterPro	IPR000705. Galactokinase. IPR022963. Galactokinase_bac. IPR019741. Galactokinase_CS. IPR019539. GalKase_gal-bd. IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006206. Mevalonate/galactokinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00849.
Pfam	PF10509. GalKase_gal_bdg. 1 hit. PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000530. Galactokinase. 1 hit.
PRINTS	PR00473. GALCTOKINASE. PR00959. MEVGALKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00131. Gal_kin. 1 hit.
PROSITE	PS00106. GALACTOKINASE. 1 hit. PS00627. GHMP_KINASES_ATP. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAL1_MYCTU

Accession

Primary (citable) accession number: P96910

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program