Reviewed, UniProtKB/Swiss-Prot P96880 (PUR6_MYCTU)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole carboxylase catalytic subunit
    EC=4.1.1.21
Alternative name(s):
    AIR carboxylase
      Short name=AIRC

Gene names

Name:	purE
Ordered Locus Names:	Rv3275c, MT3375
ORF Names:	MTCY71.15c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This subunit can alone transform AIR to CAIR, but in association with purK, which possesses an ATPase activity, an enzyme complex is produced which is capable of converting AIR to CAIR efficiently under physiological condition By similarity.
Catalytic activity	5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homooctamer By similarity.
Sequence similarities	Belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Phosphoribosylaminoimidazole carboxylase catalytic subunit		PRO_0000074977
Sites
	Binding site	16	1	Substrate By similarity
	Binding site	19	1	Substrate By similarity
	Binding site	46	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96880-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 18D5F357FA7BC8D1	FASTA	174	17,675
	10 20 30 40 50 60 MTPAGERPRV GVIMGSDSDW PVMADAAAAL AEFDIPAEVR VVSAHRTPEA MFSYARGAAE 70 80 90 100 110 120 RGLEVIIAGA GGAAHLPGMV AAATPLPVIG VPVPLGRLDG LDSLLSIVQM PAGVPVATVS 130 140 150 160 170 IGGAGNAGLL AVRMLGAANP QLRARIVAFQ DRLADVVAAK DAELQRLAGK LTRD

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842582 Genomic DNA. Translation: CAB07078.1. AE000516 Genomic DNA. Translation: AAK47716.1.
PIR	D70979.
RefSeq	NP_217792.1. NP_337902.1.
3D structure databases
HSSP	HSSP built from PDB template 1O4V based on UniProtKB Q9WYS7.
ModBase	Search...
Genome annotation databases
GeneID	888721. 922446.
GenomeReviews	Gene locus MT3375 in contig AE000516_GR. Gene locus Rv3275c in contig AL123456_GR.
KEGG	mtc:MT3375. mtu:Rv3275c.
TIGR	MT3375.
Organism-specific databases
TubercuList	Rv3275c.
Phylogenomic databases
HOGENOM	P96880.
OMA	GVVMGSS.
Enzyme and pathway databases
BRENDA	4.1.1.21. 809.
Family and domain databases
InterPro	IPR000031. AIR_COase_core. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
PANTHER	PTHR23046. AIR_carboxyl. 1 hit.
Pfam	PF00731. AIRC. 1 hit. [Graphical view]
ProDom	PD002193. AIR_carboxyl. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01162. purE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR6_MYCTU

Accession

Primary (citable) accession number: P96880

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	November 3, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)