P96871 (RMLD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
dTDP-4-dehydrorhamnose reductase
EC=1.1.1.133

Alternative name(s):
dTDP-4-keto-L-rhamnose reductase
dTDP-6-deoxy-L-lyxo-4-hexulose reductase
dTDP-6-deoxy-L-mannose dehydrogenase
dTDP-L-rhamnose synthase

Gene names

Name:	rmlD
Synonyms:	strL
Ordered Locus Names:	Rv3266c, MT3366

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4-hexulose to yield dTDP-L-rhamnose. Involved in the biosynthesis of the dTDP-L-rhamnose which is a component of the critical linker, D-N-acetylglucosamine-L-rhamnose disaccharide, which connects the galactan region of arabinogalactan to peptidoglycan via a phosphodiester linkage. Ref.3
Catalytic activity	dTDP-6-deoxy-beta-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-beta-L-mannose + NADPH. Ref.3
Pathway	Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the dTDP-4-dehydrorhamnose reductase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dTDP-rhamnose biosynthetic process Inferred from direct assay PubMed 10521660PubMed 19969466. Source: MTBBASE extracellular polysaccharide biosynthetic process Inferred from genetic interaction Ref.3. Source: UniProtKB
Molecular_function	dTDP-4-dehydrorhamnose reductase activity Inferred from genetic interaction Ref.3. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	dTDP-4-dehydrorhamnose reductase		PRO_0000395352
Regions
	Nucleotide binding	12 – 16	5	NAD By similarity
	Nucleotide binding	16 – 17	2	NADP By similarity
	Nucleotide binding	35 – 36	2	NAD By similarity
	Nucleotide binding	41 – 42	2	NAD/NADP By similarity
	Nucleotide binding	62 – 65	4	NAD By similarity
	Nucleotide binding	63 – 65	3	NADP By similarity
	Nucleotide binding	132 – 136	5	NAD/NADP By similarity
	Region	104 – 105	2	Substrate binding By similarity
Sites
	Binding site	157	1	Substrate; via amide nitrogen By similarity
	Binding site	158	1	NAD; via amide nitrogen By similarity
	Binding site	256	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96871 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: F8D73C1674599E09	FASTA	304	32,045
	10 20 30 40 50 60 MAGRSERLVI TGAGGQLGSH LTAQAAREGR DMLALTSSQW DITDPAAAER IIRHGDVVIN 70 80 90 100 110 120 CAAYTDVDGA ESNEAVAYAV NATGPQHLAR ACARVGARLI HVSTDYVFDG DFGGAEPRPY 130 140 150 160 170 180 EPTDETAPQG VYARSKLAGE QAVLAAFPEA AVVRTAWVYT GGTGKDFVAV MRRLAAGHGR 190 200 210 220 230 240 VDVVDDQTGS PTYVADLAEA LLALADAGVR GRVLHAANEG VVSRFGQARA VFEECGADPQ 250 260 270 280 290 300 RVRPVSSAQF PRPAPRSSYS ALSSRQWALA GLTPLRHWRS ALATALAAPA NSTSIDRRLP STRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Formation of dTDP-rhamnose is essential for growth of mycobacteria." Ma Y., Pan F., McNeil M. J. Bacteriol. 184:3392-3395(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN DTDP-RHAMNOSE BIOSYNTHESIS, CATALYTIC ACTIVITY. Strain: ATCC 25618 / H37Rv.
[4]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842582 Genomic DNA. Translation: CAB07093.1. AE000516 Genomic DNA. Translation: AAK47707.1. AL123456 Genomic DNA. Translation: CCP46085.1.
PIR	C70978.
RefSeq	NP_217783.1. NC_000962.3. NP_337893.1. NC_002755.2. YP_006516743.1. NC_018143.1.
3D structure databases
HSSP	HSSP built from PDB template 1N2S based on UniProtKB P26392.
ProteinModelPortal	P96871.
SMR	P96871. Positions 7-282.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3266c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK47707; AAK47707; MT3366.
GeneID	13318089. 888704. 922434.
KEGG	mtc:MT3366. mtu:Rv3266c. mtv:RVBD_3266c.
PATRIC	18129154. VBIMycTub22151_3673.
Organism-specific databases
TubercuList	Rv3266c.
Phylogenomic databases
eggNOG	COG1091.
HOGENOM	HOG000227711.
KO	K00067.
OMA	GSPTYVG.
ProtClustDB	CLSK792390.
Enzyme and pathway databases
UniPathway	UPA00124.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR005913. dTDP_dehydrorham_reduct. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF04321. RmlD_sub_bind. 1 hit. [Graphical view]
TIGRFAMs	TIGR01214. rmlD. 1 hit.
ProtoNet	Search...
Other
BindingDB	P96871.
ChEMBL	CHEMBL1938225.

Entry information

Entry name

RMLD_MYCTU

Accession

Primary (citable) accession number: P96871
Secondary accession number(s): L0TEU1, Q7D5T1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program