P96861 (Y3579_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized tRNA/rRNA methyltransferase Rv3579c/MT3685
EC=2.1.1.-

Gene names

Ordered Locus Names:

Rv3579c, MT3685

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the RNA methyltransferase TrmH family.
Sequence caution	The sequence AAK48043.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA methylation Inferred from electronic annotation. Source: GOC RNA processing Inferred from electronic annotation. Source: InterPro growth Inferred from mutant phenotype PubMed 12657046. Source: MTBBASE
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Uncharacterized tRNA/rRNA methyltransferase Rv3579c/MT3685		PRO_0000379581
Sites
	Binding site	261	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	281	1	S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	290	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96861 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: C02D12F75646D71D	FASTA	322	34,010
	10 20 30 40 50 60 MPGNSRRRGA VRKSGTKKGA GVGSGGQRRR GLEGRGPTPP AHLRPHHPAA KRARAQPRRP 70 80 90 100 110 120 VKRADETETV LGRNPVLECL RAGVPATALY VALGTEADER LTECVARAAD SGIAIVELLR 130 140 150 160 170 180 ADLDRMTANH LHQGIALQVP PYNYAHPDDL LAAALDQPPA LLVALDNLSD PRNLGAIVRS 190 200 210 220 230 240 VAAFGGHGVL IPQRRSASVT AVAWRTSAGA AARIPVARAT NLTRTLKGWA DRGVRVIGLD 250 260 270 280 290 300 AGGGTALDDV DGTDSLVVVV GSEGKGLSRL VRQNCDEVVS IPMAAQAESL NASVAAGVVL 310 320 AEIARQRRRP REPREQTQNR MI

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842583 Genomic DNA. Translation: CAB07153.1. AE000516 Genomic DNA. Translation: AAK48043.1. Different initiation. AL123456 Genomic DNA. Translation: CCP46402.1.
PIR	A70607.
RefSeq	NP_218096.1. NC_000962.3. NP_338229.1. NC_002755.2. YP_006517069.1. NC_018143.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZ0 based on UniProtKB P63177.
ProteinModelPortal	P96861.
SMR	P96861. Positions 70-307.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3579c.
Proteomic databases
PRIDE	P96861.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK48043; AAK48043; MT3685.
GeneID	13317188. 888317. 926535.
KEGG	mtc:MT3685. mtu:Rv3579c. mtv:RVBD_3579c.
PATRIC	18129859. VBIMycTub22151_4024.
Organism-specific databases
TubercuList	Rv3579c.
Phylogenomic databases
eggNOG	COG0566.
HOGENOM	HOG000218798.
KO	K03218.
OMA	NITDPRN.
ProtClustDB	CLSK792577.
Family and domain databases
InterPro	IPR004441. rRNA_MeTrfase_TrmH. IPR001537. SpoU_MeTrfase. IPR013123. SpoU_subst-bd. [Graphical view]
Pfam	PF00588. SpoU_methylase. 1 hit. PF08032. SpoU_sub_bind. 1 hit. [Graphical view]
SMART	SM00967. SpoU_sub_bind. 1 hit. [Graphical view]
TIGRFAMs	TIGR00186. rRNA_methyl_3. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Y3579_MYCTU

Accession

Primary (citable) accession number: P96861
Secondary accession number(s): L0TEK2, Q7D586

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program