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Protein

Glucose-6-phosphate isomerase

Gene

pgi

Organism
Mycobacterium smegmatis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Pathway:iglycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi), Glucose-6-phosphate isomerase (pgi)
  3. 6-phosphofructokinase (pfkA)
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei353 – 3531Proton donorBy similarity
Active sitei384 – 3841By similarity
Active sitei510 – 5101By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgi
OrganismiMycobacterium smegmatis
Taxonomic identifieri1772 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 549549Glucose-6-phosphate isomerasePRO_0000180676Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246196.MSMEG_5541.

Structurei

3D structure databases

ProteinModelPortaliP96803.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GPI family.Curated

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96803-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSADITETPA WQALSDHHAE IGDRHLTELF ADDPARGTEL ALTVGDLYID
60 70 80 90 100
YSKHRVTRRT LDLLVDLARA AGLEERRDAM FAGEHINTSE DRAVLHTALR
110 120 130 140 150
LPRDAKLVVD GQDVVADVHD VLDRMGDFTD RLRSGEWTGA TGERITTVVN
160 170 180 190 200
IGIGGSDLGP VMVYDALRHY ADAGISARFV SNVDPADLVA KLDGLEPAKT
210 220 230 240 250
LFIVASKTFS TLETLTNATA ARRWLTDALG DAAVAKHSSR CPPTRSWSTK
260 270 280 290 300
FGINTDNMFG FWDWVGGRYS VDSAIGLSVM AVIGKERFAE FLAGFHIVDE
310 320 330 340 350
HFRTAPLHQN APALLGLIGL WYSNFFGAQS RAVLPYSNDL SRFAAYLQQL
360 370 380 390 400
TMESNGKSVR ADGTPVSTDT GEIFWGEPGT NGQHAFYQLL HQGTRLVPAD
410 420 430 440 450
FIGFSQPTDD LPTADGTGSM HDLLMSNFFA QTQVLAFGKT ADAIASEGTP
460 470 480 490 500
ADVVPHKVMP GNRPTTSILA TKLTPSVVGQ LIALYEHQVF TEGVIWGIDS
510 520 530 540
FDQWGVELGK TQAKALLPVL TGDKSPAAQS DTSTDALVRR YRTERGRPA
Length:549
Mass (Da):59,673
Last modified:October 17, 2006 - v2
Checksum:iEC850980E57EF9BC
GO

Sequence cautioni

The sequence AAB52545.1 differs from that shown. Reason: Frameshift at position 101. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88433 Genomic DNA. Translation: AAB52545.1. Frameshift.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U88433 Genomic DNA. Translation: AAB52545.1. Frameshift.

3D structure databases

ProteinModelPortaliP96803.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_5541.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.

Family and domain databases

Gene3Di1.10.1390.10. 1 hit.
HAMAPiMF_00473. G6P_isomerase.
InterProiIPR001672. G6P_Isomerase.
IPR023096. G6P_Isomerase_C.
IPR018189. Phosphoglucose_isomerase_CS.
[Graphical view]
PANTHERiPTHR11469. PTHR11469. 1 hit.
PfamiPF00342. PGI. 1 hit.
[Graphical view]
PRINTSiPR00662. G6PISOMERASE.
PROSITEiPS00765. P_GLUCOSE_ISOMERASE_1. 1 hit.
PS00174. P_GLUCOSE_ISOMERASE_2. 1 hit.
PS51463. P_GLUCOSE_ISOMERASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Interruption of the phosphoglucose isomerase gene results in glucose auxotrophy in Mycobacterium smegmatis."
    Tuckman D., Donnelly R.J., Zhao F.X., Jacobs W.R. Jr., Connell N.D.
    J. Bacteriol. 179:2724-2730(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: mc(2)743.

Entry informationi

Entry nameiG6PI_MYCSM
AccessioniPrimary (citable) accession number: P96803
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: October 17, 2006
Last modified: June 24, 2015
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.