Reviewed,
UniProtKB/Swiss-Prot P96796 (HDRE_METBF)
Last modified
January 19, 2010.
Version 59.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: CoB--CoM heterodisulfide reductase subunit E EC=1.8.98.1 | ||||
| Gene names |
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| Organism | Methanosarcina barkeri (strain Fusaro / DSM 804) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 269797 [NCBI] | ||||
| Taxonomic lineage | Archaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina |
Protein attributes
| Sequence length | 263 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B). HdrE may be responsible for anchoring the complex to the membrane. Ref.3 |
| Catalytic activity | Coenzyme B + coenzyme M + methanophenazine = N-(7-((2-sulfoethyl)dithio)heptanoyl)-O(3)-phospho-L-threonine + dihydromethanophenazine. |
| Pathway | |
| Subunit structure | The heterodisulfide reductase is composed of two subunits; hdrD and hdrE. Ref.3 |
| Subcellular location | Cell membrane; Multi-pass membrane protein Potential. |
| Sequence similarities | Belongs to the hdrE family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Methanogenesis |
| Cellular component | Cell membrane Membrane |
| Domain | Transmembrane |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | methanogenesis Ref.3 Inferred from direct assay. Source: UniProtKB oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Ref.1 Non-traceable author statement. Source: UniProtKB nitrate reductase complexInferred from electronic annotation. Source: InterPro plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | CoB--CoM heterodisulfide reductase activity Ref.3 Inferred from direct assay. Source: UniProtKB nitrate reductase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 263 | 263 | CoB--CoM heterodisulfide reductase subunit E | PRO_0000150083 | |||||
Regions | |||||||||
| Transmembrane | 18 – 38 | 21 | Potential | ||||||
| Transmembrane | 108 – 128 | 21 | Potential | ||||||
| Transmembrane | 150 – 170 | 21 | Potential | ||||||
| Transmembrane | 184 – 204 | 21 | Potential | ||||||
| Transmembrane | 221 – 241 | 21 | Potential | ||||||
Experimental info | |||||||||
| Sequence conflict | 1 – 2 | 2 | MS → K AA sequence Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Heterodisulfide reductase from methanol-grown cells of Methanosarcina barkeri is not a flavoenzyme." Kuenkel A., Vaupel M., Heim S., Thauer R.K., Hedderich R. Eur. J. Biochem. 244:226-234(1997) [PubMed: 9063468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24. |
| [2] | "The Methanosarcina barkeri genome: comparative analysis with Methanosarcina acetivorans and Methanosarcina mazei reveals extensive rearrangement within methanosarcinal genomes." Maeder D.L., Anderson I., Brettin T.S., Bruce D.C., Gilna P., Han C.S., Lapidus A., Metcalf W.W., Saunders E., Tapia R., Sowers K.R. J. Bacteriol. 188:7922-7931(2006) [PubMed: 16980466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [3] | "Purification of a two-subunit cytochrome-b-containing heterodisulfide reductase from methanol-grown Methanosarcina barkeri." Heiden S., Hedderich R., Setzke E., Thauer R.K. Eur. J. Biochem. 221:855-861(1994) [PubMed: 8174566] [Abstract] Cited for: FUNCTION, SUBUNIT, PROTEIN SEQUENCE OF 1-24. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y09870 Genomic DNA. Translation: CAA70996.1. CP000099 Genomic DNA. Translation: AAZ70544.1. Different initiation. |
| PIR | S43469. |
| RefSeq | YP_305124.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | P96796. |
Protein family/group databases | |
| TCDB | 3.D.7.1.1. H2:heterodisulfide oxidoreductase (HHO) family. |
Genome annotation databases | |
| GeneID | 3624317. |
| GenomeReviews | Gene locus Mbar_A1598 in contig CP000099_GR. |
| KEGG | mba:Mbar_A1598. |
| NMPDR | fig|269797.3.peg.1906. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| eggNOG | arNOG07835. |
| HOGENOM | HBG538650. |
Enzyme and pathway databases | |
| BioCyc | MBAR269797:MBAR_A1598-MONOMER. |
| BRENDA | 1.8.98.1. 9. |
Family and domain databases | |
| InterPro | IPR003816. Nitrate_red_gam. [Graphical view] |
| Pfam | PF02665. Nitrate_red_gam. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | HDRE_METBF | ||||||||
| Accession | Primary (citable) accession number: P96796 Secondary accession number(s): Q46C48, Q9UWK1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


