P96786 (FLID_HELPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Flagellar hook-associated protein 2
Short name=HAP2

Alternative name(s):
Filament cap protein
Flagellar cap protein

Gene names

Name:	fliD
Ordered Locus Names:	HP_0752

Organism

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) [Reference proteome] [HAMAP]

Taxonomic identifier

85962 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › Helicobacter pylori

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end. Essential to colonize and establish infection in gastric mucosa as a result of its essential role in motility. Has effect on flaA gene transcription.
Subunit structure	Homopentamer By similarity.
Subcellular location	Secreted. Bacterial flagellum.
Sequence similarities	Belongs to the FliD family.

Ontologies

Keywords
Cellular component	Bacterial flagellum Secreted
Domain	Coiled coil
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro flagellum assembly Inferred from electronic annotation. Source: InterPro
Cellular_component	bacterial-type flagellum Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 685	684	Flagellar hook-associated protein 2		PRO_0000177019
Regions
	Coiled coil	618 – 647	30	Potential
Natural variations
	Natural variant	59	1	A → S in strain: KCTC 0217BP.
	Natural variant	86	1	S → N in strain: KCTC 0217BP.
	Natural variant	91	1	V → A in strain: KCTC 0217BP.
	Natural variant	193	1	V → I in strain: KCTC 0217BP.
	Natural variant	231	1	N → S in strain: KCTC 0217BP.
	Natural variant	258	1	M → I in strain: KCTC 0217BP.
	Natural variant	270	1	N → D in strain: KCTC 0217BP.
	Natural variant	363	1	I → V in strain: KCTC 0217BP.
	Natural variant	368	1	A → S in strain: KCTC 0217BP.
	Natural variant	373	1	S → N in strain: KCTC 0217BP.
	Natural variant	422	1	N → G in strain: KCTC 0217BP.
Experimental info
	Sequence conflict	654 – 685	32	ERFAA…AAKKN → DVLPLMIAKSLKPIKNSIPC K in AAD07800. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P96786 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 12D4F56473D047F6	FASTA	685	74,131
	10 20 30 40 50 60 MAIGSLSSLG LGSKVLNYDV IDKLKDADEK ALIAPLDKKM EQNVEKQKAL VEIKTLLSAL 70 80 90 100 110 120 KGPVKTLSDY STYISRKSNV TGDALSASVG VGVPIQDIKV DVQNLAQGDI NELGAKFSSR 130 140 150 160 170 180 DDIFSQVDTT LKFYTQNKDY AVNIKAGMTL GDVAQSITDA TNGEVMGIVM KTGGNDPYQL 190 200 210 220 230 240 MVNTKNTGED NRVYFGSHLQ STLTNKNALS LGVDGSGKSE VSLNLKGADG NMHEVPIMLE 250 260 270 280 290 300 LPESASIKQK NTAIQKAMEQ ALENDPNFKN LIANGDISID TLHGGESLII NDRRGGNIEV 310 320 330 340 350 360 KGSKAKELGF LQTTTQESDL LKSSRTIKEG KLEGVVSLNG QKLDLSALTK ESNTSEENTD 370 380 390 400 410 420 AIIQAINAKE GLSAFKNAEG KLVINSKTGM LTIKGEDALG KASLKDLGLN AGMVQSYEAS 430 440 450 460 470 480 QNTLFMSKNL QKASDSAFTY NGVSITRPTN EVNDVISGVN ITLEQTTEPN KPAIISVSRD 490 500 510 520 530 540 NQAIIDSLTE FVKAYNELIP KLDEDTRYDA DTKIAGIFNG VGDIRAIRSS LNNVFSYSVH 550 560 570 580 590 600 TDNGVESLMK YGLSLDDKGV MSLDEAKLSS ALNSNPKATQ DFFYGSDSKD MGGREIHQEG 610 620 630 640 650 660 IFSKFNQVIA NLIDGGNAKL KIYEDSLDRD AKSLTKDKEN AQELLKTRYN IMAERFAAYD 670 680 SQISKANQKF NSVQMMIDQA AAKKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the Helicobacter pylori fliD gene, an essential factor in flagellar structure and motility." Kim J.S., Chang J.H., Chung S.I., Yum J.S. J. Bacteriol. 181:6969-6976(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16, CHARACTERIZATION. Strain: KCTC 0217BP.
[2]	"The complete genome sequence of the gastric pathogen Helicobacter pylori." Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G., Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A., Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N., Loftus B.J., Richardson D.L., Dodson R.J. , Khalak H.G., Glodek A., McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E., Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D., Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S., Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C. Nature 388:539-547(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700392 / 26695.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82981 Genomic DNA. Translation: AAC35999.1. AE000511 Genomic DNA. Translation: AAD07800.1.
PIR	H64613.
RefSeq	NP_207545.1. NC_000915.1. YP_006934668.1. NC_018939.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3401N.
MINT	MINT-176105.
STRING	85962.HP0752.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD07800; AAD07800; HP_0752.
GeneID	13869937. 900004.
KEGG	heo:C694_03870. hpy:HP0752.
PATRIC	20592793. VBIHelPyl33062_0785.
Phylogenomic databases
eggNOG	COG1345.
KO	K02407.
OMA	YNIMAER.
ProtClustDB	PRK08453.
Family and domain databases
InterPro	IPR010809. FliD_C. IPR003481. FliD_N. [Graphical view]
Pfam	PF07195. FliD_C. 1 hit. PF02465. FliD_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FLID_HELPY

Accession

Primary (citable) accession number: P96786

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 78 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program