Reviewed, UniProtKB/Swiss-Prot P96771 (PURA_AGGAC)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:

purA

Organism

Aggregatibacter actinomycetemcomitans (Actinobacillus actinomycetemcomitans) (Haemophilus actinomycetemcomitans)

Taxonomic identifier

714 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Aggregatibacter

Protein attributes

Sequence length	259 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 259	›259	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000095139
Regions
	Nucleotide binding	3 – 9	7	GTP Potential
Sites
	Active site	131	1	By similarity
	Active site	138	1	By similarity
	Metal binding	4	1	Magnesium By similarity
	Metal binding	31	1	Magnesium; via carbonyl oxygen By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P96771-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: E64198443875D6C6	FASTA	259	28,479
	10 20 30 40 50 60 QWGDEGKGKI VDLLTDRVKY VVRYQGGRGA GHTLILNGEK TVLRLIPSGI LRDNVTCLIG 70 80 90 100 110 120 NGVVLSPAAL MQEMSELENR GVNVRDRLLI SEACPLILPY HVAMDHAREA RWAKRPIRAT 130 140 150 160 170 180 GRGIGPAYED KVARRGLRVG DLFDRAAFAE KLKNILDYYN FQLVNYYKVE PVDYQKTLDD 190 200 210 220 230 240 VFAVADIITG MVADITTILD TARKNGDNIL FEGAQGTMLD IDHGTYPYVT SSNTAGGVAS 250 GSGFGPRNLD YVLGIIKAY

References

[1]	"Codon usage in Actinobacillus actinomycetemcomitans." Kaplan J.B., Fine D.H. FEMS Microbiol. Lett. 163:31-36(1998) [PubMed: 9631542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CU1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U89525 Genomic DNA. Translation: AAC46412.1.
3D structure databases
SMR	P96771. Positions 1-259.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.4.4. 164755.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_AGGAC

Accession

Primary (citable) accession number: P96771

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)