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P96718

- YWQF_BACSU

UniProt

P96718 - YWQF_BACSU

Protein

UDP-glucose 6-dehydrogenase YwqF

Gene

ywqF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of UDP-glucose into UDP-glucuronate, one of the precursors of teichuronic acid.1 Publication

    Catalytic activityi

    UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.

    Enzyme regulationi

    Competitively inhibited by UDP-glucose. Activated by phosphorylation, which may increase affinity for NAD+; inhibited by dephosphorylation.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei260 – 2601By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi2 – 1918NADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. polysaccharide biosynthetic process Source: InterPro
    2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBSUB:BSU36230-MONOMER.
    UniPathwayiUPA00038; UER00491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase YwqF (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Gene namesi
    Name:ywqF
    Ordered Locus Names:BSU36230
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU36230.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi10 – 101Y → F: 30-fold decrease in activity but no decrease in the overall phosphorylation level. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 440440UDP-glucose 6-dehydrogenase YwqFPRO_0000253341Add
    BLAST

    Post-translational modificationi

    Phosphorylated on tyrosine residue(s). Phosphorylated by YwqD and dephosphorylated by YwqE in vitro.2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP96718.

    PTM databases

    PhosSiteiP0604162.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU36230.

    Structurei

    3D structure databases

    ProteinModelPortaliP96718.
    SMRiP96718. Positions 1-429.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004.
    HOGENOMiHOG000153773.
    KOiK00012.
    OMAiCEMVGAN.
    OrthoDBiEOG6ZSP7N.
    PhylomeDBiP96718.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P96718-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNITVIGTGY VGLVTGVSLS EIGHHVTCID IDAHKIDEMR KGISPIFEPG    50
    LEELMRKNTA DGRLNFETSY EKGLAQADII FIAVGTPQKS DGHANLEQIT 100
    DAAKRIAKHV KRDTVVVTKS TVPVGTNDLI NGLITEHLAE PVSISVASNP 150
    EFLREGSAIY DTFHGDRIVI GTADEKTANT LEELFRPFQI PIYQTDIRSA 200
    EMIKYASNAF LATKISFINE ISNICEKVGA DIEAVAYGMG QDKRIGSQFL 250
    KAGIGYGGSC FPKDTNALVQ IAGNVEHDFE LLKSVIKVNN NQQAMLVDKA 300
    LNRLGGVTGK TIALLGLSFK PNTDDMREAP SIVIADRLAA LDARIRAYDP 350
    IAVSHAKHVL PQAVEYKETI EEAVKGSDAV MILTDWADIK QFPLAAYQDL 400
    METPLIFDGR NCYTLDEALA AGVEYYSVGR KAVVPSGAIQ 440
    Length:440
    Mass (Da):47,784
    Last modified:May 1, 1997 - v1
    Checksum:i1ADA9454EBE1F645
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z92952 Genomic DNA. Translation: CAB07444.1.
    AL009126 Genomic DNA. Translation: CAB15640.1.
    PIRiA70067.
    RefSeqiNP_391504.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15640; CAB15640; BSU36230.
    GeneIDi936890.
    KEGGibsu:BSU36230.
    PATRICi18979266. VBIBacSub10457_3795.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z92952 Genomic DNA. Translation: CAB07444.1 .
    AL009126 Genomic DNA. Translation: CAB15640.1 .
    PIRi A70067.
    RefSeqi NP_391504.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P96718.
    SMRi P96718. Positions 1-429.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU36230.

    PTM databases

    PhosSitei P0604162.

    Proteomic databases

    PaxDbi P96718.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15640 ; CAB15640 ; BSU36230 .
    GeneIDi 936890.
    KEGGi bsu:BSU36230.
    PATRICi 18979266. VBIBacSub10457_3795.

    Organism-specific databases

    GenoListi BSU36230.

    Phylogenomic databases

    eggNOGi COG1004.
    HOGENOMi HOG000153773.
    KOi K00012.
    OMAi CEMVGAN.
    OrthoDBi EOG6ZSP7N.
    PhylomeDBi P96718.

    Enzyme and pathway databases

    UniPathwayi UPA00038 ; UER00491 .
    BioCyci BSUB:BSU36230-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028357. UDPglc_DH_bac.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500134. UDPglc_DH_bac. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases."
      Mijakovic I., Poncet S., Boel G., Maze A., Gillet S., Jamet E., Decottignies P., Grangeasse C., Doublet P., Le Marechal P., Deutscher J.
      EMBO J. 22:4709-4718(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, ENZYME REGULATION.
    4. "How tyrosine phosphorylation affects the UDP-glucose dehydrogenase activity of Bacillus subtilis YwqF."
      Mijakovic I., Petranovic D., Deutscher J.
      J. Mol. Microbiol. Biotechnol. 8:19-25(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF TYR-10, PHOSPHORYLATION.

    Entry informationi

    Entry nameiYWQF_BACSU
    AccessioniPrimary (citable) accession number: P96718
    Secondary accession number(s): Q795B4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 17, 2006
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3