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P96717 (YWQE_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein phosphatase YwqE

EC=3.1.3.48
Gene names
Name:ywqE
Ordered Locus Names:BSU36240
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length254 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Dephosphorylates the phosphotyrosine-containing proteins YwqD, YwqF and Ssb. Ref.4

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Cofactor

Manganese.

Enzyme regulation

Inhibited by vanadate and sodium pyrophosphate. Not inhibited by sodium fluoride.

Sequence similarities

Belongs to the CpsB/CapC family.

Biophysicochemical properties

pH dependence:

Optimally active at alkaline pHs. Activity increases with increasing pHs.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 254254Tyrosine-protein phosphatase YwqE
PRO_0000057893

Experimental info

Mutagenesis31D → A: Large decrease in activity. Ref.3
Mutagenesis51H → A: Large decrease in activity. Ref.3
Mutagenesis71H → A: Large decrease in activity.
Mutagenesis421H → A: Large decrease in activity. Ref.3
Mutagenesis1361H → A: Large decrease in activity. Ref.3
Mutagenesis1941D → A: Large decrease in activity. Ref.3
Mutagenesis1961H → A: Large decrease in activity. Ref.3

Secondary structure

.............................................. 254
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P96717 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: E2625AE51E0E9A94

FASTA25428,958
        10         20         30         40         50         60 
MIDIHCHILP AMDDGAGDSA DSIEMARAAV RQGIRTIIAT PHHNNGVYKN EPAAVREAAD 

        70         80         90        100        110        120 
QLNKRLIKED IPLHVLPGQE IRIYGEVEQD LAKRQLLSLN DTKYILIEFP FDHVPRYAEQ 

       130        140        150        160        170        180 
LFYDLQLKGY IPVIAHPERN REIRENPSLL YHLVEKGAAS QITSGSLAGI FGKQLKAFSL 

       190        200        210        220        230        240 
RLVEANLIHF VASDAHNVKT RNFHTQEALY VLEKEFGSEL PYMLTENAEL LLRNQTIFRQ 

       250 
PPQPVKRRKL FGFF 

« Hide

References

« Hide 'large scale' references
[1]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"In vitro characterization of the Bacillus subtilis protein tyrosine phosphatase YwqE."
Mijakovic I., Musumeci L., Tautz L., Petranovic D., Edwards R.A., Jensen P.R., Mustelin T., Deutscher J., Bottini N.
J. Bacteriol. 187:3384-3390(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, MUTAGENESIS OF ASP-3; HIS-5; HIS-42; HIS-136; ASP-194 AND HIS-196.
[4]"Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine."
Mijakovic I., Petranovic D., Macek B., Cepo T., Mann M., Davies J., Jensen P.R., Vujaklija D.
Nucleic Acids Res. 34:1588-1596(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z92952 Genomic DNA. Translation: CAB07456.1.
AL009126 Genomic DNA. Translation: CAB15641.1.
PIRH70066.
RefSeqNP_391505.1. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3QY6X-ray1.80A1-254[»]
3QY7X-ray1.62A1-254[»]
ProteinModelPortalP96717.
SMRP96717. Positions 1-237.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU36240.

Protein family/group databases

PptaseDBP3D0505166.

Proteomic databases

PaxDbP96717.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15641; CAB15641; BSU36240.
GeneID936893.
KEGGbsu:BSU36240.
PATRIC18979268. VBIBacSub10457_3796.

Organism-specific databases

GenoListBSU36240.

Phylogenomic databases

eggNOGCOG4464.
HOGENOMHOG000007624.
KOK01104.
OMATPHTLNG.
OrthoDBEOG6PKFDD.
PhylomeDBP96717.

Enzyme and pathway databases

BioCycBSUB:BSU36240-MONOMER.
SABIO-RKP96717.

Family and domain databases

InterProIPR016667. Caps_polysacc_synth_CpsB/CapC.
IPR004013. PHP_C.
IPR016195. Pol/histidinol_Pase-like.
[Graphical view]
PfamPF02811. PHP. 1 hit.
[Graphical view]
PIRSFPIRSF016557. Caps_synth_CpsB. 1 hit.
SUPFAMSSF89550. SSF89550. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP96717.

Entry information

Entry nameYWQE_BACSU
AccessionPrimary (citable) accession number: P96717
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList