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P96716

- YWQD_BACSU

UniProt

P96716 - YWQD_BACSU

Protein

Tyrosine-protein kinase YwqD

Gene

ywqD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    May be involved in the regulation of capsular polysaccharide biosynthesis. Autophosphorylates in vitro. Phosphorylates and activates in vitro two UDP-glucose dehydrogenases, YwqF and TuaD, as well as the DNA-binding proteins Ssb and SsbB.2 Publications

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei81 – 811Required for activity
    Sitei83 – 831Required for activity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. enzyme regulator activity Source: InterPro
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. capsule polysaccharide biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Capsule biogenesis/degradation, Exopolysaccharide synthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU36250-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase YwqD (EC:2.7.10.2)
    Gene namesi
    Name:ywqD
    Ordered Locus Names:BSU36250
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU36250.

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi81 – 811D → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi83 – 831D → A: Abolishes ATPase activity. 1 Publication
    Mutagenesisi225 – 2251Y → F: Prevents phosphorylation and increases ATPase activity; when associated with F-227 and F-228. 1 Publication
    Mutagenesisi227 – 2271Y → F: Prevents phosphorylation and increases ATPase activity; when associated with F-225 and F-228. 1 Publication
    Mutagenesisi228 – 2281Y → F: Prevents phosphorylation and increases ATPase activity; when associated with F-225 and F-227. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237Tyrosine-protein kinase YwqDPRO_0000217242Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei228 – 2281Phosphotyrosine; by autocatalysis1 Publication

    Post-translational modificationi

    Autophosphorylated in vitro, which inhibits ATPase activity. Dephosphorylated by YwqE in vitro.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP96716.

    PTM databases

    PhosSiteiP0310110.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU36250.

    Structurei

    3D structure databases

    ProteinModelPortaliP96716.
    SMRiP96716. Positions 6-230.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CpsD/CapB family.Curated

    Phylogenomic databases

    eggNOGiCOG0489.
    HOGENOMiHOG000004334.
    KOiK00903.
    OMAiNSVIRES.
    OrthoDBiEOG69D3H6.
    PhylomeDBiP96716.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR025669. AAA_dom.
    IPR005702. EPS_synthesis.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF13614. AAA_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01007. eps_fam. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P96716-1 [UniParc]FASTAAdd to Basket

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    MALRKNRGSR MQRNVIAMTE PKSLNSEQYR TIRTNIEFAS VDRQMKSVMI    50
    TSACPGEGKS TTAANLAVVF AQQGKKVLLI DADLRKPTVH TAFFLENTVG 100
    LTSVLLKKSS MEQAVQASNE KHLDVLTSGP IPPNPAELLS SKWMKELAYE 150
    ACAAYDMVIF DTPPILAVAD AQILGNVADG SVLVISSGKT EKEQAAKAKE 200
    ALATCKSKLL GAIMNGKKLS KHSEYGYYGT KDNFMQK 237
    Length:237
    Mass (Da):25,790
    Last modified:May 1, 1997 - v1
    Checksum:i40BE35B5E3FF97D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z92952 Genomic DNA. Translation: CAB07457.1.
    AL009126 Genomic DNA. Translation: CAB15642.1.
    PIRiG70066.
    RefSeqiNP_391506.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15642; CAB15642; BSU36250.
    GeneIDi936896.
    KEGGibsu:BSU36250.
    PATRICi18979270. VBIBacSub10457_3797.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z92952 Genomic DNA. Translation: CAB07457.1 .
    AL009126 Genomic DNA. Translation: CAB15642.1 .
    PIRi G70066.
    RefSeqi NP_391506.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P96716.
    SMRi P96716. Positions 6-230.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU36250.

    PTM databases

    PhosSitei P0310110.

    Proteomic databases

    PaxDbi P96716.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15642 ; CAB15642 ; BSU36250 .
    GeneIDi 936896.
    KEGGi bsu:BSU36250.
    PATRICi 18979270. VBIBacSub10457_3797.

    Organism-specific databases

    GenoListi BSU36250.

    Phylogenomic databases

    eggNOGi COG0489.
    HOGENOMi HOG000004334.
    KOi K00903.
    OMAi NSVIRES.
    OrthoDBi EOG69D3H6.
    PhylomeDBi P96716.

    Enzyme and pathway databases

    BioCyci BSUB:BSU36250-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR025669. AAA_dom.
    IPR005702. EPS_synthesis.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF13614. AAA_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01007. eps_fam. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Transmembrane modulator-dependent bacterial tyrosine kinase activates UDP-glucose dehydrogenases."
      Mijakovic I., Poncet S., Boel G., Maze A., Gillet S., Jamet E., Decottignies P., Grangeasse C., Doublet P., Le Marechal P., Deutscher J.
      EMBO J. 22:4709-4718(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-81; ASP-83; TYR-225; TYR-227 AND TYR-228, PHOSPHORYLATION AT TYR-228.
    4. "Bacterial single-stranded DNA-binding proteins are phosphorylated on tyrosine."
      Mijakovic I., Petranovic D., Macek B., Cepo T., Mann M., Davies J., Jensen P.R., Vujaklija D.
      Nucleic Acids Res. 34:1588-1596(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: 168.

    Entry informationi

    Entry nameiYWQD_BACSU
    AccessioniPrimary (citable) accession number: P96716
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2003
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3