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P96711

- ARAR_BACSU

UniProt

P96711 - ARAR_BACSU

Protein

Arabinose metabolism transcriptional repressor

Gene

araR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (03 Apr 2002)
      Previous versions | rss
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    Functioni

    Transcriptional repressor of the arabinose utilization genes. Also regulates its own expression. Binds to two sequences within the promoters of the araABDLMNPQ-abfA operon and the araE gene, and to one sequence in the araR promoter.

    Enzyme regulationi

    Binding to DNA is inhibited by L-arabinose.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi30 – 4920H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU33970-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arabinose metabolism transcriptional repressor
    Gene namesi
    Name:araR
    Synonyms:araC, yvbS
    Ordered Locus Names:BSU33970
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU33970. [Micado]

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 362362Arabinose metabolism transcriptional repressorPRO_0000050620Add
    BLAST

    Proteomic databases

    PaxDbiP96711.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU33970.

    Structurei

    Secondary structure

    1
    362
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1714
    Helixi30 – 378
    Helixi41 – 5313
    Beta strandi56 – 605
    Turni61 – 633
    Beta strandi64 – 674
    Beta strandi81 – 877
    Beta strandi89 – 913
    Helixi94 – 10714
    Beta strandi111 – 1166
    Helixi121 – 13313
    Beta strandi137 – 1415
    Helixi153 – 1619
    Beta strandi166 – 1716
    Beta strandi180 – 1834
    Helixi185 – 19814
    Beta strandi203 – 21210
    Helixi213 – 22816
    Helixi235 – 2373
    Beta strandi238 – 2414
    Helixi243 – 2464
    Helixi249 – 26012
    Turni261 – 2633
    Beta strandi267 – 2704
    Helixi274 – 28613
    Turni291 – 2944
    Beta strandi296 – 2983
    Helixi305 – 3084
    Beta strandi309 – 3113
    Beta strandi314 – 3174
    Helixi321 – 33515
    Beta strandi345 – 3473
    Beta strandi350 – 3523

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3TB6X-ray2.21A/B71-362[»]
    4EGYX-ray2.30A/B1-68[»]
    4EGZX-ray2.30A/B1-68[»]
    4H0EX-ray1.97A/B1-68[»]
    ProteinModelPortaliP96711.
    SMRiP96711. Positions 2-360.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 7070HTH gntR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH gntR-type DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1609.
    HOGENOMiHOG000235268.
    OrthoDBiEOG64BQ72.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR028082. Peripla_BP_I.
    IPR000524. Tscrpt_reg_HTH_GntR.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00392. GntR. 1 hit.
    [Graphical view]
    PRINTSiPR00035. HTHGNTR.
    SMARTiSM00345. HTH_GNTR. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.
    PROSITEiPS50949. HTH_GNTR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P96711-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPKYAQVKE EISSWINQGK ILPDQKIPTE NELMQQFGVS RHTIRKAIGD    50
    LVSQGLLYSV QGGGTFVASR SAKSALHSNK TIGVLTTYIS DYIFPSIIRG 100
    IESYLSEQGY SMLLTSTNNN PDNERRGLEN LLSQHIDGLI VEPTKSALQT 150
    PNIGYYLNLE KNGIPFAMIN ASYAELAAPS FTLDDVKGGM MAAEHLLSLG 200
    HTHMMGIFKA DDTQGVKRMN GFIQAHRERE LFPSPDMIVT FTTEEKESKL 250
    LEKVKATLEK NSKHMPTAIL CYNDEIALKV IDMLREMDLK VPEDMSIVGY 300
    DDSHFAQISE VKLTSVKHPK SVLGKAAAKY VIDCLEHKKP KQEDVIFEPE 350
    LIIRQSARKL NE 362
    Length:362
    Mass (Da):40,488
    Last modified:April 3, 2002 - v3
    Checksum:i679DC8FEE69FB421
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98354 Genomic DNA. Translation: CAA66999.1.
    AL009126 Genomic DNA. Translation: CAB15402.2.
    PIRiD69588.

    Genome annotation databases

    EnsemblBacteriaiCAB15402; CAB15402; BSU33970.
    PATRICi18978794. VBIBacSub10457_3560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98354 Genomic DNA. Translation: CAA66999.1 .
    AL009126 Genomic DNA. Translation: CAB15402.2 .
    PIRi D69588.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3TB6 X-ray 2.21 A/B 71-362 [» ]
    4EGY X-ray 2.30 A/B 1-68 [» ]
    4EGZ X-ray 2.30 A/B 1-68 [» ]
    4H0E X-ray 1.97 A/B 1-68 [» ]
    ProteinModelPortali P96711.
    SMRi P96711. Positions 2-360.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU33970.

    Proteomic databases

    PaxDbi P96711.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15402 ; CAB15402 ; BSU33970 .
    PATRICi 18978794. VBIBacSub10457_3560.

    Organism-specific databases

    GenoListi BSU33970. [Micado ]

    Phylogenomic databases

    eggNOGi COG1609.
    HOGENOMi HOG000235268.
    OrthoDBi EOG64BQ72.

    Enzyme and pathway databases

    BioCyci BSUB:BSU33970-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR028082. Peripla_BP_I.
    IPR000524. Tscrpt_reg_HTH_GntR.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00392. GntR. 1 hit.
    [Graphical view ]
    PRINTSi PR00035. HTHGNTR.
    SMARTi SM00345. HTH_GNTR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    PROSITEi PS50949. HTH_GNTR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene."
      Sa-Nogueira I.M.G., Mota L.J.
      J. Bacteriol. 179:1598-1608(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "Mode of action of AraR, the key regulator of L-arabinose metabolism in Bacillus subtilis."
      Mota L.J., Tavares P., Sa-Nogueira I.M.G.
      Mol. Microbiol. 33:476-489(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-10, CHARACTERIZATION.
    4. "Control of the arabinose regulon in Bacillus subtilis by AraR in vivo: crucial roles of operators, cooperativity, and DNA looping."
      Mota L.J., Sarmento L.M., Sa-Nogueira I.M.G.
      J. Bacteriol. 183:4190-4201(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: 168.

    Entry informationi

    Entry nameiARAR_BACSU
    AccessioniPrimary (citable) accession number: P96711
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2002
    Last sequence update: April 3, 2002
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3