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Protein

mRNA interferase EndoA

Gene

ndoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.2 Publications

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. endonuclease activity Source: UniProtKB-KW
  3. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease, Toxin

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciBSUB:BSU04660-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA interferase EndoA (EC:3.1.-.-)
Alternative name(s):
Endoribonuclease EndoA
Toxin EndoA
mRNA interferase MazF-bs
Short name:
MazF-bs
Gene namesi
Name:ndoA
Synonyms:mazF, ydcE
Ordered Locus Names:BSU04660
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU04660. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 116116mRNA interferase EndoAPRO_0000201900Add
BLAST

Proteomic databases

PaxDbiP96622.

Interactioni

Subunit structurei

Homodimer. Forms a complex with antitoxin EndoAI in which the toxin activity is inhibited.1 Publication

Protein-protein interaction databases

STRINGi224308.BSU04660.

Structurei

Secondary structure

1
116
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Beta strandi23 – 297Combined sources
Helixi33 – 386Combined sources
Beta strandi40 – 5011Combined sources
Beta strandi59 – 624Combined sources
Helixi64 – 674Combined sources
Beta strandi73 – 8412Combined sources
Helixi85 – 873Combined sources
Beta strandi88 – 947Combined sources
Helixi97 – 11014Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NE8X-ray2.10A2-116[»]
4MDXX-ray1.50A/B1-116[»]
4ME7X-ray2.92A/B/C/D2-116[»]
ProteinModelPortaliP96622.
SMRiP96622. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96622.

Family & Domainsi

Sequence similaritiesi

Belongs to the PemK/MazF family.Curated

Phylogenomic databases

eggNOGiCOG2337.
HOGENOMiHOG000290184.
InParanoidiP96622.
KOiK11680.
OMAiNQYSPTT.
OrthoDBiEOG64BQCS.
PhylomeDBiP96622.

Family and domain databases

Gene3Di2.30.30.110. 1 hit.
InterProiIPR003477. PemK-like.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]
PfamiPF02452. PemK. 1 hit.
[Graphical view]
PIRSFiPIRSF033490. MazF. 1 hit.
SUPFAMiSSF50118. SSF50118. 1 hit.

Sequencei

Sequence statusi: Complete.

P96622-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIVKRGDVYF ADLSPVVGSE QGGVRPVLVI QNDIGNRFSP TAIVAAITAQ
60 70 80 90 100
IQKAKLPTHV EIDAKRYGFE RDSVILLEQI RTIDKQRLTD KITHLDDEMM
110
DKVDEALQIS LALIDF
Length:116
Mass (Da):12,978
Last modified:May 1, 1997 - v1
Checksum:iFE89144E62BD2B7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001488 Genomic DNA. Translation: BAA19303.1.
AL009126 Genomic DNA. Translation: CAB12273.1.
PIRiC69773.
RefSeqiNP_388347.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12273; CAB12273; BSU04660.
GeneIDi939935.
KEGGibsu:BSU04660.
PATRICi18972516. VBIBacSub10457_0486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001488 Genomic DNA. Translation: BAA19303.1.
AL009126 Genomic DNA. Translation: CAB12273.1.
PIRiC69773.
RefSeqiNP_388347.1. NC_000964.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NE8X-ray2.10A2-116[»]
4MDXX-ray1.50A/B1-116[»]
4ME7X-ray2.92A/B/C/D2-116[»]
ProteinModelPortaliP96622.
SMRiP96622. Positions 1-116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.BSU04660.

Proteomic databases

PaxDbiP96622.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12273; CAB12273; BSU04660.
GeneIDi939935.
KEGGibsu:BSU04660.
PATRICi18972516. VBIBacSub10457_0486.

Organism-specific databases

GenoListiBSU04660. [Micado]

Phylogenomic databases

eggNOGiCOG2337.
HOGENOMiHOG000290184.
InParanoidiP96622.
KOiK11680.
OMAiNQYSPTT.
OrthoDBiEOG64BQCS.
PhylomeDBiP96622.

Enzyme and pathway databases

BioCyciBSUB:BSU04660-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP96622.

Family and domain databases

Gene3Di2.30.30.110. 1 hit.
InterProiIPR003477. PemK-like.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]
PfamiPF02452. PemK. 1 hit.
[Graphical view]
PIRSFiPIRSF033490. MazF. 1 hit.
SUPFAMiSSF50118. SSF50118. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
    Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor."
    Pellegrini O., Mathy N., Gogos A., Shapiro L., Condon C.
    Mol. Microbiol. 56:1139-1148(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN MRNA INTERFERASE, INHIBITION BY ENDOAI.
    Strain: 168.
  4. "Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase."
    Park J.H., Yamaguchi Y., Inouye M.
    FEBS Lett. 585:2526-2532(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN MRNA INTERFERASE, INHIBITION BY ENDOAI, EXPRESSION IN E.COLI.
    Strain: 168.
  5. "Crystal structure of YdcE protein from Bacillus subtilis."
    Gogos A., Mu H., Bahna F., Gomez C.A., Shapiro L.
    Proteins 53:320-322(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-116, SUBUNIT.

Entry informationi

Entry nameiENDOA_BACSU
AccessioniPrimary (citable) accession number: P96622
Secondary accession number(s): Q797K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.