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P96622 (ENDOA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
mRNA interferase EndoA

EC=3.1.-.-
Alternative name(s):
Endoribonuclease EndoA
Toxin EndoA
mRNA interferase MazF-bs
Short name=MazF-bs
Gene names
Name:ndoA
Synonyms:mazF, ydcE
Ordered Locus Names:BSU04660
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length116 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli. Ref.3 Ref.4

Subunit structure

Homodimer. Forms a complex with antitoxin EndoAI in which the toxin activity is inhibited. Ref.5

Sequence similarities

Belongs to the PemK/MazF family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 116116mRNA interferase EndoA
PRO_0000201900

Secondary structure

................... 116
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P96622 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: FE89144E62BD2B7C

FASTA11612,978
        10         20         30         40         50         60 
MIVKRGDVYF ADLSPVVGSE QGGVRPVLVI QNDIGNRFSP TAIVAAITAQ IQKAKLPTHV 

        70         80         90        100        110 
EIDAKRYGFE RDSVILLEQI RTIDKQRLTD KITHLDDEMM DKVDEALQIS LALIDF 

« Hide

References

« Hide 'large scale' references
[1]"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor."
Pellegrini O., Mathy N., Gogos A., Shapiro L., Condon C.
Mol. Microbiol. 56:1139-1148(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN MRNA INTERFERASE, INHIBITION BY ENDOAI.
Strain: 168.
[4]"Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase."
Park J.H., Yamaguchi Y., Inouye M.
FEBS Lett. 585:2526-2532(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS AN MRNA INTERFERASE, INHIBITION BY ENDOAI, EXPRESSION IN E.COLI.
Strain: 168.
[5]"Crystal structure of YdcE protein from Bacillus subtilis."
Gogos A., Mu H., Bahna F., Gomez C.A., Shapiro L.
Proteins 53:320-322(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-116, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB001488 Genomic DNA. Translation: BAA19303.1.
AL009126 Genomic DNA. Translation: CAB12273.1.
PIRC69773.
RefSeqNP_388347.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1NE8X-ray2.10A2-116[»]
4MDXX-ray1.50A/B1-116[»]
4ME7X-ray2.92A/B/C/D2-116[»]
ProteinModelPortalP96622.
SMRP96622. Positions 1-116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU04660.

Proteomic databases

PaxDbP96622.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12273; CAB12273; BSU04660.
GeneID939935.
KEGGbsu:BSU04660.
PATRIC18972516. VBIBacSub10457_0486.

Organism-specific databases

GenoListBSU04660. [Micado]

Phylogenomic databases

eggNOGCOG2337.
HOGENOMHOG000290184.
KOK07171.
OMANQYSPTT.
OrthoDBEOG64BQCS.
ProtClustDBCLSK2752318.

Enzyme and pathway databases

BioCycBSUB:BSU04660-MONOMER.

Family and domain databases

Gene3D2.30.30.110. 1 hit.
InterProIPR003477. PemK.
IPR011067. Plasmid_toxin/cell-grow_inhib.
[Graphical view]
PfamPF02452. PemK. 1 hit.
[Graphical view]
PIRSFPIRSF033490. MazF. 1 hit.
SUPFAMSSF50118. SSF50118. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP96622.

Entry information

Entry nameENDOA_BACSU
AccessionPrimary (citable) accession number: P96622
Secondary accession number(s): Q797K4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 2, 2005
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList