Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P96622

- ENDOA_BACSU

UniProt

P96622 - ENDOA_BACSU

Protein

mRNA interferase EndoA

Gene

ndoA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Toxic component of a toxin-antitoxin (TA) module. Has specificity for UACAU sequences, claving after the first U and yielding cleavage products with 3' phosphate and 5' hydroxyl groups. Overexpression is toxic for cell growth, probably by inhibiting protein synthesis through the cleavage of single-stranded RNA. The toxicity is reversed by the antitoxin EndoAI. Toxin activity cannot be inhibited by MazE from E.coli.2 Publications

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. endonuclease activity Source: UniProtKB-KW
    3. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease, Toxin

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU04660-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    mRNA interferase EndoA (EC:3.1.-.-)
    Alternative name(s):
    Endoribonuclease EndoA
    Toxin EndoA
    mRNA interferase MazF-bs
    Short name:
    MazF-bs
    Gene namesi
    Name:ndoA
    Synonyms:mazF, ydcE
    Ordered Locus Names:BSU04660
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU04660. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 116116mRNA interferase EndoAPRO_0000201900Add
    BLAST

    Proteomic databases

    PaxDbiP96622.

    Interactioni

    Subunit structurei

    Homodimer. Forms a complex with antitoxin EndoAI in which the toxin activity is inhibited.1 Publication

    Protein-protein interaction databases

    STRINGi224308.BSU04660.

    Structurei

    Secondary structure

    1
    116
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 126
    Beta strandi23 – 297
    Helixi33 – 386
    Beta strandi40 – 5011
    Beta strandi59 – 624
    Helixi64 – 674
    Beta strandi73 – 8412
    Helixi85 – 873
    Beta strandi88 – 947
    Helixi97 – 11014

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1NE8X-ray2.10A2-116[»]
    4MDXX-ray1.50A/B1-116[»]
    4ME7X-ray2.92A/B/C/D2-116[»]
    ProteinModelPortaliP96622.
    SMRiP96622. Positions 1-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP96622.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PemK/MazF family.Curated

    Phylogenomic databases

    eggNOGiCOG2337.
    HOGENOMiHOG000290184.
    KOiK07171.
    OMAiNQYSPTT.
    OrthoDBiEOG64BQCS.
    PhylomeDBiP96622.

    Family and domain databases

    Gene3Di2.30.30.110. 1 hit.
    InterProiIPR003477. PemK-like.
    IPR011067. Plasmid_toxin/cell-grow_inhib.
    [Graphical view]
    PfamiPF02452. PemK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF033490. MazF. 1 hit.
    SUPFAMiSSF50118. SSF50118. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P96622-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIVKRGDVYF ADLSPVVGSE QGGVRPVLVI QNDIGNRFSP TAIVAAITAQ    50
    IQKAKLPTHV EIDAKRYGFE RDSVILLEQI RTIDKQRLTD KITHLDDEMM 100
    DKVDEALQIS LALIDF 116
    Length:116
    Mass (Da):12,978
    Last modified:May 1, 1997 - v1
    Checksum:iFE89144E62BD2B7C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001488 Genomic DNA. Translation: BAA19303.1.
    AL009126 Genomic DNA. Translation: CAB12273.1.
    PIRiC69773.
    RefSeqiNP_388347.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB12273; CAB12273; BSU04660.
    GeneIDi939935.
    KEGGibsu:BSU04660.
    PATRICi18972516. VBIBacSub10457_0486.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB001488 Genomic DNA. Translation: BAA19303.1 .
    AL009126 Genomic DNA. Translation: CAB12273.1 .
    PIRi C69773.
    RefSeqi NP_388347.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1NE8 X-ray 2.10 A 2-116 [» ]
    4MDX X-ray 1.50 A/B 1-116 [» ]
    4ME7 X-ray 2.92 A/B/C/D 2-116 [» ]
    ProteinModelPortali P96622.
    SMRi P96622. Positions 1-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU04660.

    Proteomic databases

    PaxDbi P96622.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12273 ; CAB12273 ; BSU04660 .
    GeneIDi 939935.
    KEGGi bsu:BSU04660.
    PATRICi 18972516. VBIBacSub10457_0486.

    Organism-specific databases

    GenoListi BSU04660. [Micado ]

    Phylogenomic databases

    eggNOGi COG2337.
    HOGENOMi HOG000290184.
    KOi K07171.
    OMAi NQYSPTT.
    OrthoDBi EOG64BQCS.
    PhylomeDBi P96622.

    Enzyme and pathway databases

    BioCyci BSUB:BSU04660-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P96622.

    Family and domain databases

    Gene3Di 2.30.30.110. 1 hit.
    InterProi IPR003477. PemK-like.
    IPR011067. Plasmid_toxin/cell-grow_inhib.
    [Graphical view ]
    Pfami PF02452. PemK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF033490. MazF. 1 hit.
    SUPFAMi SSF50118. SSF50118. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
      Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
      Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "The Bacillus subtilis ydcDE operon encodes an endoribonuclease of the MazF/PemK family and its inhibitor."
      Pellegrini O., Mathy N., Gogos A., Shapiro L., Condon C.
      Mol. Microbiol. 56:1139-1148(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN MRNA INTERFERASE, INHIBITION BY ENDOAI.
      Strain: 168.
    4. "Bacillus subtilis MazF-bs (EndoA) is a UACAU-specific mRNA interferase."
      Park J.H., Yamaguchi Y., Inouye M.
      FEBS Lett. 585:2526-2532(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS AN MRNA INTERFERASE, INHIBITION BY ENDOAI, EXPRESSION IN E.COLI.
      Strain: 168.
    5. "Crystal structure of YdcE protein from Bacillus subtilis."
      Gogos A., Mu H., Bahna F., Gomez C.A., Shapiro L.
      Proteins 53:320-322(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-116, SUBUNIT.

    Entry informationi

    Entry nameiENDOA_BACSU
    AccessioniPrimary (citable) accession number: P96622
    Secondary accession number(s): Q797K4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 2, 2005
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3