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Protein

Holo-[acyl-carrier-protein] synthase

Gene

acpS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the 4'-phosphopantetheine moiety from coenzyme A to a Ser of fatty acid acyl-carrier-protein ACP. Also modifies the D-alanyl carrier protein but fails to recognize PCP and AcpK, an acyl carrier protein of secondary metabolism.1 Publication

Catalytic activityi

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium1
Metal bindingi58Magnesium1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU04620-MONOMER.
SABIO-RKP96618.

Names & Taxonomyi

Protein namesi
Recommended name:
Holo-[acyl-carrier-protein] synthaseUniRule annotation (EC:2.7.8.7UniRule annotation)
Short name:
Holo-ACP synthaseUniRule annotation
Alternative name(s):
4'-phosphopantetheinyl transferase AcpSUniRule annotation
Gene namesi
Name:acpSUniRule annotation
Synonyms:ydcB
Ordered Locus Names:BSU04620
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi2I → A: 6% of wild-type activity. 1 Publication1
Mutagenesisi5I → R: Loss of activity; no trimer formation. 1 Publication1
Mutagenesisi113Q → E: 14% of wild-type activity. 1 Publication1
Mutagenesisi113Q → R: Loss of activity; no trimer formation. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL4734.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001756131 – 121Holo-[acyl-carrier-protein] synthaseAdd BLAST121

Proteomic databases

PaxDbiP96618.
PRIDEiP96618.

Interactioni

Subunit structurei

Homotrimer.1 Publication

Protein-protein interaction databases

IntActiP96618. 1 interactor.
STRINGi224308.Bsubs1_010100002618.

Chemistry databases

BindingDBiP96618.

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 11Combined sources10
Helixi12 – 21Combined sources10
Helixi25 – 29Combined sources5
Helixi32 – 38Combined sources7
Helixi43 – 63Combined sources21
Beta strandi68 – 71Combined sources4
Helixi74 – 76Combined sources3
Beta strandi78 – 81Combined sources4
Beta strandi87 – 91Combined sources5
Turni92 – 94Combined sources3
Beta strandi95 – 105Combined sources11
Beta strandi107 – 117Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F7LX-ray1.50A1-121[»]
1F7TX-ray1.80A/B/C/D/E/F2-121[»]
1F80X-ray2.30A/B/C2-121[»]
ProteinModelPortaliP96618.
SMRiP96618.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96618.

Family & Domainsi

Sequence similaritiesi

Belongs to the P-Pant transferase superfamily. AcpS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41082V8. Bacteria.
COG0736. LUCA.
HOGENOMiHOG000014315.
InParanoidiP96618.
KOiK00997.
OMAiHGVSFQD.
PhylomeDBiP96618.

Family and domain databases

Gene3Di3.90.470.20. 1 hit.
HAMAPiMF_00101. AcpS. 1 hit.
InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
IPR002582. ACPS.
IPR004568. Ppantetheine-prot_Trfase_dom.
[Graphical view]
PfamiPF01648. ACPS. 1 hit.
[Graphical view]
ProDomiPD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56214. SSF56214. 1 hit.
TIGRFAMsiTIGR00516. acpS. 1 hit.
TIGR00556. pantethn_trn. 1 hit.

Sequencei

Sequence statusi: Complete.

P96618-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYGIGLDIT ELKRIASMAG RQKRFAERIL TRSELDQYYE LSEKRKNEFL
60 70 80 90 100
AGRFAAKEAF SKAFGTGIGR QLSFQDIEIR KDQNGKPYII CTKLSQAAVH
110 120
VSITHTKEYA AAQVVIERLS S
Length:121
Mass (Da):13,718
Last modified:May 1, 1997 - v1
Checksum:i6C10401DA7116701
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001488 Genomic DNA. Translation: BAA19299.1.
AL009126 Genomic DNA. Translation: CAB12269.1.
PIRiH69772.
RefSeqiNP_388343.1. NC_000964.3.
WP_003234281.1. NZ_JNCM01000031.1.

Genome annotation databases

EnsemblBacteriaiCAB12269; CAB12269; BSU04620.
GeneIDi938194.
KEGGibsu:BSU04620.
PATRICi18972508. VBIBacSub10457_0482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001488 Genomic DNA. Translation: BAA19299.1.
AL009126 Genomic DNA. Translation: CAB12269.1.
PIRiH69772.
RefSeqiNP_388343.1. NC_000964.3.
WP_003234281.1. NZ_JNCM01000031.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F7LX-ray1.50A1-121[»]
1F7TX-ray1.80A/B/C/D/E/F2-121[»]
1F80X-ray2.30A/B/C2-121[»]
ProteinModelPortaliP96618.
SMRiP96618.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP96618. 1 interactor.
STRINGi224308.Bsubs1_010100002618.

Chemistry databases

BindingDBiP96618.
ChEMBLiCHEMBL4734.

Proteomic databases

PaxDbiP96618.
PRIDEiP96618.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12269; CAB12269; BSU04620.
GeneIDi938194.
KEGGibsu:BSU04620.
PATRICi18972508. VBIBacSub10457_0482.

Phylogenomic databases

eggNOGiENOG41082V8. Bacteria.
COG0736. LUCA.
HOGENOMiHOG000014315.
InParanoidiP96618.
KOiK00997.
OMAiHGVSFQD.
PhylomeDBiP96618.

Enzyme and pathway databases

BioCyciBSUB:BSU04620-MONOMER.
SABIO-RKP96618.

Miscellaneous databases

EvolutionaryTraceiP96618.

Family and domain databases

Gene3Di3.90.470.20. 1 hit.
HAMAPiMF_00101. AcpS. 1 hit.
InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
IPR002582. ACPS.
IPR004568. Ppantetheine-prot_Trfase_dom.
[Graphical view]
PfamiPF01648. ACPS. 1 hit.
[Graphical view]
ProDomiPD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56214. SSF56214. 1 hit.
TIGRFAMsiTIGR00516. acpS. 1 hit.
TIGR00556. pantethn_trn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACPS_BACSU
AccessioniPrimary (citable) accession number: P96618
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.