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P96614 (CSHA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DEAD-box ATP-dependent RNA helicase CshA
EC=3.6.4.13
Gene names
Name:cshA
Synonyms:ydbR
Ordered Locus Names:BSU04580
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length494 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May work in conjunction with the cold shock proteins to ensure proper initiation of transcription at low and optimal temperatures. In vitro, unwinds dsRNA in both 5'- and 3'-directions and shows RNA-dependent ATPase activity. Required for vegetative growth at 37 degrees Celsius and more important for growth at 22 degrees Celsius. Ref.5

Catalytic activity

ATP + H2O = ADP + phosphate.

Subcellular location

Cytoplasm › nucleoid. Note: Shows transcription-dependent localization at subcellular sites surrounding the nucleoid. Associated with free 50S ribosomal subunit, 70S ribosome and polysomes. Ref.4 Ref.5

Induction

Induced by cold shock. Ref.3 Ref.5

Miscellaneous

The presence of CshA or CshB is essential for viability.

Sequence similarities

Belongs to the DEAD box helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Sequence caution

The sequence BAA19295.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB12265.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processRNA secondary structure unwinding

Inferred from direct assay Ref.4. Source: UniProtKB

response to cold

Inferred from genetic interaction Ref.5. Source: UniProtKB

   Cellular componentbacterial nucleoid

Inferred from direct assay Ref.5. Source: UniProtKB

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from direct assay Ref.4. Source: UniProtKB

ATP-dependent RNA helicase activity

Inferred from direct assay Ref.4. Source: UniProtKB

RNA binding

Inferred from direct assay Ref.4. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 494494DEAD-box ATP-dependent RNA helicase CshA
PRO_0000280054

Regions

Domain34 – 204171Helicase ATP-binding
Domain215 – 375161Helicase C-terminal
Nucleotide binding47 – 548ATP By similarity
Motif3 – 3129Q motif
Motif152 – 1554DEAD box

Sequences

Sequence LengthMass (Da)Tools
P96614 [UniParc].

Last modified March 6, 2007. Version 2.
Checksum: A44C5EAE314E46ED

FASTA49455,330
        10         20         30         40         50         60 
MTITFQDFNL SSDLMKAINR MGFEEATPIQ AQTIPLGLSN KDVIGQAQTG TGKTAAFGIP 

        70         80         90        100        110        120 
LVEKINPESP NIQAIVIAPT RELAIQVSEE LYKIGQDKRA KVLPIYGGQD IGRQIRALKK 

       130        140        150        160        170        180 
NPNIIVGTPG RLLDHINRRT IRLNNVNTVV MDEADEMLNM GFIDDIESIL SNVPSEHQTL 

       190        200        210        220        230        240 
LFSATMPAPI KRIAERFMTE PEHVKVKAKE MTVSNIQQFY LEVQERKKFD TLTRLLDIQS 

       250        260        270        280        290        300 
PELAIVFGRT KRRVDELAEA LNLRGYAAEG IHGDLTQAKR MVALRKFKEG AIEVLVATDV 

       310        320        330        340        350        360 
AARGLDISGV THVYNFDVPQ DPESYVHRIG RTGRAGKTGM AMTFITPREK SMLRAIEQTT 

       370        380        390        400        410        420 
KRKMDRMKEP TLDEALEGQQ QVTVERLRTT ISENNLNFYM TAAAELLEDH DAVTVVAAAI 

       430        440        450        460        470        480 
KMATKEPDDT PVRLTDEAPM VSKRYKNQRS SKRRDGQGGG YRGGKGKSNN RSSYDKKRSN 

       490 
DRRSSGDRRQ KKSY

« Hide

References

« Hide 'large scale' references
[1]"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis."
Beckering C.L., Steil L., Weber M.H.W., Voelker U., Marahiel M.A.
J. Bacteriol. 184:6395-6402(2002) [PubMed: 12399512] [Abstract]
Cited for: INDUCTION BY COLD SHOCK.
Strain: 168 / JH642.
[4]"Bacillus subtilis DEAD protein YdbR possesses ATPase, RNA binding, and RNA unwinding activities."
Ando Y., Nakamura K.
Biosci. Biotechnol. Biochem. 70:1606-1615(2006) [PubMed: 16861794] [Abstract]
Cited for: CHARACTERIZATION, SUBCELLULAR LOCATION.
[5]"Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis."
Hunger K., Beckering C.L., Wiegeshoff F., Graumann P.L., Marahiel M.A.
J. Bacteriol. 188:240-248(2006) [PubMed: 16352840] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INDUCTION.
Strain: 168 / JH642.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB001488 Genomic DNA. Translation: BAA19295.1. Different initiation.
AL009126 Genomic DNA. Translation: CAB12265.1. Different initiation.
PIRD69772.
RefSeqNP_388339.1. NC_000964.3.

3D structure databases

HSSPHSSP built from PDB template 1HV8 based on UniProtKB Q58083.
ProteinModelPortalP96614.
SMRP96614. Positions 3-423.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBACT00000000739; EBBACP00000000739; EBBACG00000000737.
GeneID938170.
GenomeReviewsGene locus BSU04580 in contig AL009126_GR.
KEGGbsu:BSU04580.
NMPDRfig|224308.1.peg.458.
PATRIC18972500. VBIBacSub10457_0478.

Organism-specific databases

GenoListBSU04580. [Micado]

Phylogenomic databases

GeneTreeEBGT00070000031896.
HOGENOMHBG737336.
PhylomeDBP96614.
ProtClustDBCLSK886742.

Enzyme and pathway databases

BioCycBSUB:BSU04580-MONOMER.

Family and domain databases

InterProIPR014001. DEAD-like_helicase.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR001650. Helicase_C.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCSHA_BACSU
AccessionPrimary (citable) accession number: P96614
Secondary accession number(s): Q797L0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 6, 2007
Last modified: January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents