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Protein

Probable C4-dicarboxylate sensor kinase

Gene

dctS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system DctS/DctR. Probably activates DctR by phosphorylation (By similarity). Essential for expression of dctP.By similarity2 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU04450-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable C4-dicarboxylate sensor kinase (EC:2.7.13.3)
Gene namesi
Name:dctS
Synonyms:ydbF
Ordered Locus Names:BSU04450
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Topological domaini33 – 172140ExtracellularSequence analysisAdd
BLAST
Transmembranei173 – 19321HelicalSequence analysisAdd
BLAST
Topological domaini194 – 535342CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 535535Probable C4-dicarboxylate sensor kinasePRO_0000074728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei336 – 3361Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP96601.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100002518.

Structurei

3D structure databases

ProteinModelPortaliP96601.
SMRiP96601. Positions 37-169, 201-312, 394-527.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini213 – 27664PASAdd
BLAST
Domaini333 – 528196Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiP96601.
KOiK11691.
OMAiIMSSRIP.
OrthoDBiEOG65BDG0.
PhylomeDBiP96601.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKKLSIRW KITILSYILV IFSFLIGGIV LIGNIQHTEE RELKKRLMNT
60 70 80 90 100
ARTVSEMTEV KEALARKKQT EAVRHAVEEI RMINEADYIV VMDMNHIRYT
110 120 130 140 150
HPVSTSIGKK SEGADEEAAF AEHIYFSEAK GEIGTAVRAF YPVKDQDLNQ
160 170 180 190 200
IGVVLVGKTL PGIADILLHL KRDIAFIVVL TLGFGLAGSF LLARHIKKQM
210 220 230 240 250
FQLEPHEIVR MYEERTATFH SMNEGVIAID NRLVITIFNE KAKQIFEVQG
260 270 280 290 300
DLIGKVIWEV LKDSRLPEIV ERNKAVYNEE IRVSGKVIMS SRIPIVMKKK
310 320 330 340 350
VIGAVAIFQD RTEAAKMAEE LTGVRNFVEA LRVQNHEHMN KLHTIAGLIQ
360 370 380 390 400
LGKSEKALQL AFQASTEQEN VTEFLHRSIQ NDAAAGLLLS KIRRGRELGI
410 420 430 440 450
AVHIDENSSL QQFPEHVDQH DIVVLLGNLI ENAFGSFETV QSEDKRIDIS
460 470 480 490 500
IEQTDDILAI LIEDNGCGIE PTHMPRLYDK GFTVNKTGGT GYGLYLVKQI
510 520 530
IDKGSGTIEV DSHAGQGTSF SIVFPMKGEE AQHGS
Length:535
Mass (Da):59,942
Last modified:May 1, 1997 - v1
Checksum:i614BDA8BE8F10CFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001488 Genomic DNA. Translation: BAA19282.1.
AL009126 Genomic DNA. Translation: CAB12252.1.
PIRiA69771.
RefSeqiNP_388326.1. NC_000964.3.
WP_003234348.1. NZ_JNCM01000031.1.

Genome annotation databases

EnsemblBacteriaiCAB12252; CAB12252; BSU04450.
GeneIDi938231.
KEGGibsu:BSU04450.
PATRICi18972470. VBIBacSub10457_0463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001488 Genomic DNA. Translation: BAA19282.1.
AL009126 Genomic DNA. Translation: CAB12252.1.
PIRiA69771.
RefSeqiNP_388326.1. NC_000964.3.
WP_003234348.1. NZ_JNCM01000031.1.

3D structure databases

ProteinModelPortaliP96601.
SMRiP96601. Positions 37-169, 201-312, 394-527.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100002518.

Proteomic databases

PaxDbiP96601.

Protocols and materials databases

DNASUi938231.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12252; CAB12252; BSU04450.
GeneIDi938231.
KEGGibsu:BSU04450.
PATRICi18972470. VBIBacSub10457_0463.

Phylogenomic databases

eggNOGiENOG4105CEQ. Bacteria.
COG3290. LUCA.
HOGENOMiHOG000241936.
InParanoidiP96601.
KOiK11691.
OMAiIMSSRIP.
OrthoDBiEOG65BDG0.
PhylomeDBiP96601.

Enzyme and pathway databases

BioCyciBSUB:BSU04450-MONOMER.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR033463. sCache_3.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
IPR016120. Sig_transdc_His_kin_SpoOB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF17203. sCache_3_2. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
SSF55890. SSF55890. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
    Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
    Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis."
    Asai K., Baik S.-H., Kasahara Y., Moriya S., Ogasawara N.
    Microbiology 146:263-271(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, GENE NAME.
    Strain: 168.
  4. "Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems."
    Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T., Fujita Y.
    J. Bacteriol. 183:7365-7370(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiDCTS_BACSU
AccessioniPrimary (citable) accession number: P96601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: May 1, 1997
Last modified: May 11, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.