Reviewed, UniProtKB/Swiss-Prot P96601 (DCTS_BACSU)

Names and origin

Protein names

Recommended name:
    Probable C4-dicarboxylate sensor kinase
    EC=2.7.13.3

Gene names

Name:	dctS
Synonyms:	ydbF
Ordered Locus Names:	BSU04450

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	535 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Member of the two-component regulatory system dctS/dctR. Probably activates dctR by phosphorylation By similarity. Essential for expression of dctP.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 histidine kinase domain. Contains 1 PAS (PER-ARNT-SIM) domain.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 535	535	Probable C4-dicarboxylate sensor kinase		PRO_0000074728
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Potential
	Topological domain	33 – 172	140	Extracellular Potential
	Transmembrane	173 – 193	21	Potential
	Topological domain	194 – 535	342	Cytoplasmic Potential
	Domain	213 – 276	64	PAS
	Domain	333 – 528	196	Histidine kinase
Amino acid modifications
	Modified residue	336	1	Phosphohistidine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96601-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 614BDA8BE8F10CFE	FASTA	535	59,942
	10 20 30 40 50 60 MNKKKLSIRW KITILSYILV IFSFLIGGIV LIGNIQHTEE RELKKRLMNT ARTVSEMTEV 70 80 90 100 110 120 KEALARKKQT EAVRHAVEEI RMINEADYIV VMDMNHIRYT HPVSTSIGKK SEGADEEAAF 130 140 150 160 170 180 AEHIYFSEAK GEIGTAVRAF YPVKDQDLNQ IGVVLVGKTL PGIADILLHL KRDIAFIVVL 190 200 210 220 230 240 TLGFGLAGSF LLARHIKKQM FQLEPHEIVR MYEERTATFH SMNEGVIAID NRLVITIFNE 250 260 270 280 290 300 KAKQIFEVQG DLIGKVIWEV LKDSRLPEIV ERNKAVYNEE IRVSGKVIMS SRIPIVMKKK 310 320 330 340 350 360 VIGAVAIFQD RTEAAKMAEE LTGVRNFVEA LRVQNHEHMN KLHTIAGLIQ LGKSEKALQL 370 380 390 400 410 420 AFQASTEQEN VTEFLHRSIQ NDAAAGLLLS KIRRGRELGI AVHIDENSSL QQFPEHVDQH 430 440 450 460 470 480 DIVVLLGNLI ENAFGSFETV QSEDKRIDIS IEQTDDILAI LIEDNGCGIE PTHMPRLYDK 490 500 510 520 530 GFTVNKTGGT GYGLYLVKQI IDKGSGTIEV DSHAGQGTSF SIVFPMKGEE AQHGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome." Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N. Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis." Asai K., Baik S.-H., Kasahara Y., Moriya S., Ogasawara N. Microbiology 146:263-271(2000) [PubMed: 10708364] [Abstract] Cited for: FUNCTION, GENE NAME. Strain: 168.
[4]	"Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems." Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T., Fujita Y. J. Bacteriol. 183:7365-7370(2001) [PubMed: 11717295] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB001488 Genomic DNA. Translation: BAA19282.1. AL009126 Genomic DNA. Translation: CAB12252.1.
PIR	A69771.
RefSeq	NP_388326.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938231.
GenomeReviews	Gene locus BSU04450 in contig AL009126_GR.
KEGG	bsu:BSU04450.
NMPDR	fig|224308.1.peg.445.
Organism-specific databases
SubtiList	BG12073. dctS. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P96601.
OMA	KIRRGKE.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0445-MON.
BRENDA	2.7.13.3. 150.
Family and domain databases
InterPro	IPR003594. ATP_bd_ATPase. IPR000014. PAS. IPR004358. Sig_transdc_His_kin-like_C. IPR005467. Sig_transdc_His_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.
Pfam	PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00344. BCTRLSENSOR.
SMART	SM00387. HATPase_c. 1 hit. SM00091. PAS. 1 hit. [Graphical view]
PROSITE	PS50109. HIS_KIN. 1 hit. PS50112. PAS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DCTS_BACSU

Accession

Primary (citable) accession number: P96601

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	May 1, 1997
Last modified:	November 3, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)