Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

dTDP-epi-vancosaminyltransferase

Gene

gtfA

Organism
Amycolatopsis orientalis (Nocardia orientalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of 4-epi-vancosamine from a TDP donor to the beta-OH-Tyr-6 of the aglycone cosubstrate in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics. Strongly prefers devancoaminyl-vancomycin (DVV) as substrate rather than the heptapeptide vancomycin aglycone (AGV). Acts downstream of GtfB.3 Publications

Catalytic activityi

dTDP-beta-L-4-epi-vancosamine + devancoaminyl-vancomycin = dTDP + chloroorienticin B.1 Publication

Pathwayi: vancomycin biosynthesis

This protein is involved in the pathway vancomycin biosynthesis, which is part of Antibiotic biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway vancomycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei127 – 1271Devancoaminyl-vancomycin; via carbonyl oxygen1 Publication
Binding sitei133 – 1331Devancoaminyl-vancomycin1 Publication
Binding sitei141 – 1411Devancoaminyl-vancomycin1 Publication
Binding sitei169 – 1691Devancoaminyl-vancomycin1 Publication
Binding sitei207 – 2071dTDP-beta-L-4-epi-vancosamine1 Publication
Binding sitei230 – 2301dTDP-beta-L-4-epi-vancosamine1 Publication

GO - Molecular functioni

  • transferase activity, transferring hexosyl groups Source: UniProtKB

GO - Biological processi

  • lipid glycosylation Source: InterPro
  • vancomycin biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BRENDAi2.4.1.311. 315.
UniPathwayiUPA00162.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-epi-vancosaminyltransferase (EC:2.4.1.311)
Alternative name(s):
Glycosyltransferase AH1
Short name:
GtfAH1
Glycosyltransferase GtfA
Gene namesi
Name:gtfA
OrganismiAmycolatopsis orientalis (Nocardia orientalis)
Taxonomic identifieri31958 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeAmycolatopsis

Pathology & Biotechi

Chemistry

DrugBankiDB00512. Vancomycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396dTDP-epi-vancosaminyltransferasePRO_0000430436Add
BLAST

Structurei

Secondary structure

1
396
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 108Combined sources
Helixi11 – 2616Combined sources
Beta strandi31 – 355Combined sources
Helixi37 – 393Combined sources
Helixi40 – 456Combined sources
Beta strandi50 – 523Combined sources
Helixi59 – 613Combined sources
Helixi72 – 743Combined sources
Helixi75 – 9016Combined sources
Beta strandi94 – 1007Combined sources
Helixi102 – 11514Combined sources
Beta strandi119 – 1257Combined sources
Helixi126 – 1283Combined sources
Helixi130 – 1323Combined sources
Helixi135 – 15925Combined sources
Helixi168 – 1736Combined sources
Turni183 – 1853Combined sources
Beta strandi193 – 1975Combined sources
Helixi211 – 2144Combined sources
Beta strandi219 – 2213Combined sources
Beta strandi224 – 2274Combined sources
Helixi235 – 24915Combined sources
Beta strandi254 – 2574Combined sources
Turni259 – 2624Combined sources
Beta strandi274 – 2774Combined sources
Helixi280 – 2845Combined sources
Beta strandi290 – 2945Combined sources
Helixi296 – 30510Combined sources
Beta strandi309 – 3124Combined sources
Helixi324 – 3318Combined sources
Beta strandi334 – 3385Combined sources
Helixi345 – 35511Combined sources
Helixi360 – 3678Combined sources
Helixi368 – 3703Combined sources
Helixi375 – 39016Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PN3X-ray2.80A/B1-396[»]
1PNVX-ray2.80A/B1-396[»]
3H4IX-ray1.30A1-214[»]
3H4TX-ray1.15A1-214[»]
ProteinModelPortaliP96558.
SMRiP96558. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96558.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 123dTDP-beta-L-4-epi-vancosamine binding1 Publication
Regioni277 – 2782dTDP-beta-L-4-epi-vancosamine binding1 Publication
Regioni293 – 2986dTDP-beta-L-4-epi-vancosamine binding1 Publication

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family.Curated

Phylogenomic databases

KOiK16440.

Family and domain databases

InterProiIPR004276. GlycoTrans_28_N.
IPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF03033. Glyco_transf_28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLITGCGS RGDTEPLVAL AARLRELGAD ARMCLPPDYV ERCAEVGVPM
60 70 80 90 100
VPVGRAVRAG AREPGELPPG AAEVVTEVVA EWFDKVPAAI EGCDAVVTTG
110 120 130 140 150
LLPAAVAVRS MAEKLGIPYR YTVLSPDHLP SEQSQAERDM YNQGADRLFG
160 170 180 190 200
DAVNSHRASI GLPPVEHLYD YGYTDQPWLA ADPVLSPLRP TDLGTVQTGA
210 220 230 240 250
WILPDERPLS AELEAFLAAG STPVYVGFGS SSRPATADAA KMAIKAVRAS
260 270 280 290 300
GRRIVLSRGW ADLVLPDDGA DCFVVGEVNL QELFGRVAAA IHHDSAGTTL
310 320 330 340 350
LAMRAGIPQI VVRRVVDNVV EQAYHADRVA ELGVGVAVDG PVPTIDSLSA
360 370 380 390
ALDTALAPEI RARATTVADT IRADGTTVAA QLLFDAVSLE KPTVPA
Length:396
Mass (Da):41,662
Last modified:May 1, 1997 - v1
Checksum:iA771471559C6CBF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84349 Genomic DNA. Translation: AAB49292.1.
AJ223998 Genomic DNA. Translation: CAA11774.1.
PIRiT30584.

Genome annotation databases

KEGGiag:CAA11774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84349 Genomic DNA. Translation: AAB49292.1.
AJ223998 Genomic DNA. Translation: CAA11774.1.
PIRiT30584.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PN3X-ray2.80A/B1-396[»]
1PNVX-ray2.80A/B1-396[»]
3H4IX-ray1.30A1-214[»]
3H4TX-ray1.15A1-214[»]
ProteinModelPortaliP96558.
SMRiP96558. Positions 1-391.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

DrugBankiDB00512. Vancomycin.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA11774.

Phylogenomic databases

KOiK16440.

Enzyme and pathway databases

UniPathwayiUPA00162.
BRENDAi2.4.1.311. 315.

Miscellaneous databases

EvolutionaryTraceiP96558.

Family and domain databases

InterProiIPR004276. GlycoTrans_28_N.
IPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF03033. Glyco_transf_28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces toyocaensis."
    Solenberg P.J., Matsushima P., Stack D.R., Wilkie S.C., Thompson R.C., Baltz R.H.
    Chem. Biol. 4:195-202(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic."
    van Wageningen A., Kirkpatrick P., Williams D., Harris B., Kershaw J., Lennard N., Jones M., Jones S., Solenberg P.
    Chem. Biol. 5:155-162(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PATHWAY.
  3. "Characterization of a regiospecific epivancosaminyl transferase GtfA and enzymatic reconstitution of the antibiotic chloroeremomycin."
    Lu W., Oberthuer M., Leimkuhler C., Tao J., Kahne D., Walsh C.T.
    Proc. Natl. Acad. Sci. U.S.A. 101:4390-4395(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, PATHWAY.
  4. "Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway."
    Mulichak A.M., Losey H.C., Lu W., Wawrzak Z., Walsh C.T., Garavito R.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:9238-9243(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) IN COMPLEX WITH VANCOMYCIN AND DTDP-BETA-L-4-EPI-VANCOSAMINE.
  5. "Chimeric glycosyltransferases for the generation of hybrid glycopeptides."
    Truman A.W., Dias M.V., Wu S., Blundell T.L., Huang F., Spencer J.B.
    Chem. Biol. 16:676-685(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS) OF 1-214 IN COMPLEX WITH UDP-2-DEOXY-2-FLUORO-GLUCOSE, FUNCTION.

Entry informationi

Entry nameiGTFA_AMYOR
AccessioniPrimary (citable) accession number: P96558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: May 1, 1997
Last modified: January 20, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In A.orientalis different glycosyltransferases are involved in biosynthesis of the vancomycin group of antibiotics. GtfA, GtfB (AC P96559) and GtfC (AC P96560) are involved in biosynthesis of antibiotic chloroeremomycin, while GtfD (AC Q9AFC7) and GtfE (AC G4V4R9) are involved in biosynthesis of vancomycin.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.