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Protein

dTDP-epi-vancosaminyltransferase

Gene

gtfA

Organism
Amycolatopsis orientalis (Nocardia orientalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of 4-epi-vancosamine from a TDP donor to the beta-OH-Tyr-6 of the aglycone cosubstrate in the biosynthesis of glycopeptide antibiotic chloroeremomycin, a member of the vancomycin group of antibiotics. Strongly prefers devancoaminyl-vancomycin (DVV) as substrate rather than the heptapeptide vancomycin aglycone (AGV). Acts downstream of GtfB.3 Publications

Catalytic activityi

dTDP-beta-L-4-epi-vancosamine + devancoaminyl-vancomycin = dTDP + chloroorienticin B.1 Publication

Pathwayi: vancomycin biosynthesis

This protein is involved in the pathway vancomycin biosynthesis, which is part of Antibiotic biosynthesis.2 Publications
View all proteins of this organism that are known to be involved in the pathway vancomycin biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei127Devancoaminyl-vancomycin; via carbonyl oxygen1 Publication1
Binding sitei133Devancoaminyl-vancomycin1 Publication1
Binding sitei141Devancoaminyl-vancomycin1 Publication1
Binding sitei169Devancoaminyl-vancomycin1 Publication1
Binding sitei207dTDP-beta-L-4-epi-vancosamine1 Publication1
Binding sitei230dTDP-beta-L-4-epi-vancosamine1 Publication1

GO - Molecular functioni

  • transferase activity, transferring hexosyl groups Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Antibiotic biosynthesis

Enzyme and pathway databases

BRENDAi2.4.1.311. 315.
UniPathwayiUPA00162.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-epi-vancosaminyltransferase (EC:2.4.1.311)
Alternative name(s):
Glycosyltransferase AH1
Short name:
GtfAH1
Glycosyltransferase GtfA
Gene namesi
Name:gtfA
OrganismiAmycolatopsis orientalis (Nocardia orientalis)
Taxonomic identifieri31958 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeAmycolatopsis

Pathology & Biotechi

Chemistry databases

DrugBankiDB00512. Vancomycin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004304361 – 396dTDP-epi-vancosaminyltransferaseAdd BLAST396

Structurei

Secondary structure

1396
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 10Combined sources8
Helixi11 – 26Combined sources16
Beta strandi31 – 35Combined sources5
Helixi37 – 39Combined sources3
Helixi40 – 45Combined sources6
Beta strandi50 – 52Combined sources3
Helixi59 – 61Combined sources3
Helixi72 – 74Combined sources3
Helixi75 – 90Combined sources16
Beta strandi94 – 100Combined sources7
Helixi102 – 115Combined sources14
Beta strandi119 – 125Combined sources7
Helixi126 – 128Combined sources3
Helixi130 – 132Combined sources3
Helixi135 – 159Combined sources25
Helixi168 – 173Combined sources6
Turni183 – 185Combined sources3
Beta strandi193 – 197Combined sources5
Helixi211 – 214Combined sources4
Beta strandi219 – 221Combined sources3
Beta strandi224 – 227Combined sources4
Helixi235 – 249Combined sources15
Beta strandi254 – 257Combined sources4
Turni259 – 262Combined sources4
Beta strandi274 – 277Combined sources4
Helixi280 – 284Combined sources5
Beta strandi290 – 294Combined sources5
Helixi296 – 305Combined sources10
Beta strandi309 – 312Combined sources4
Helixi324 – 331Combined sources8
Beta strandi334 – 338Combined sources5
Helixi345 – 355Combined sources11
Helixi360 – 367Combined sources8
Helixi368 – 370Combined sources3
Helixi375 – 390Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PN3X-ray2.80A/B1-396[»]
1PNVX-ray2.80A/B1-396[»]
3H4IX-ray1.30A1-214[»]
3H4TX-ray1.15A1-214[»]
ProteinModelPortaliP96558.
SMRiP96558.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96558.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 12dTDP-beta-L-4-epi-vancosamine binding1 Publication3
Regioni277 – 278dTDP-beta-L-4-epi-vancosamine binding1 Publication2
Regioni293 – 298dTDP-beta-L-4-epi-vancosamine binding1 Publication6

Sequence similaritiesi

Belongs to the glycosyltransferase 28 family.Curated

Phylogenomic databases

KOiK16440.

Family and domain databases

InterProiIPR004276. GlycoTrans_28_N.
IPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF03033. Glyco_transf_28. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLITGCGS RGDTEPLVAL AARLRELGAD ARMCLPPDYV ERCAEVGVPM
60 70 80 90 100
VPVGRAVRAG AREPGELPPG AAEVVTEVVA EWFDKVPAAI EGCDAVVTTG
110 120 130 140 150
LLPAAVAVRS MAEKLGIPYR YTVLSPDHLP SEQSQAERDM YNQGADRLFG
160 170 180 190 200
DAVNSHRASI GLPPVEHLYD YGYTDQPWLA ADPVLSPLRP TDLGTVQTGA
210 220 230 240 250
WILPDERPLS AELEAFLAAG STPVYVGFGS SSRPATADAA KMAIKAVRAS
260 270 280 290 300
GRRIVLSRGW ADLVLPDDGA DCFVVGEVNL QELFGRVAAA IHHDSAGTTL
310 320 330 340 350
LAMRAGIPQI VVRRVVDNVV EQAYHADRVA ELGVGVAVDG PVPTIDSLSA
360 370 380 390
ALDTALAPEI RARATTVADT IRADGTTVAA QLLFDAVSLE KPTVPA
Length:396
Mass (Da):41,662
Last modified:May 1, 1997 - v1
Checksum:iA771471559C6CBF5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84349 Genomic DNA. Translation: AAB49292.1.
AJ223998 Genomic DNA. Translation: CAA11774.1.
PIRiT30584.

Genome annotation databases

KEGGiag:CAA11774.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84349 Genomic DNA. Translation: AAB49292.1.
AJ223998 Genomic DNA. Translation: CAA11774.1.
PIRiT30584.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PN3X-ray2.80A/B1-396[»]
1PNVX-ray2.80A/B1-396[»]
3H4IX-ray1.30A1-214[»]
3H4TX-ray1.15A1-214[»]
ProteinModelPortaliP96558.
SMRiP96558.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

DrugBankiDB00512. Vancomycin.

Protein family/group databases

CAZyiGT1. Glycosyltransferase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:CAA11774.

Phylogenomic databases

KOiK16440.

Enzyme and pathway databases

UniPathwayiUPA00162.
BRENDAi2.4.1.311. 315.

Miscellaneous databases

EvolutionaryTraceiP96558.

Family and domain databases

InterProiIPR004276. GlycoTrans_28_N.
IPR002213. UDP_glucos_trans.
[Graphical view]
PANTHERiPTHR11926. PTHR11926. 1 hit.
PfamiPF03033. Glyco_transf_28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGTFA_AMYOR
AccessioniPrimary (citable) accession number: P96558
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 2014
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

In A.orientalis different glycosyltransferases are involved in biosynthesis of the vancomycin group of antibiotics. GtfA, GtfB (AC P96559) and GtfC (AC P96560) are involved in biosynthesis of antibiotic chloroeremomycin, while GtfD (AC Q9AFC7) and GtfE (AC G4V4R9) are involved in biosynthesis of vancomycin.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.