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Protein

Melibiose permease

Gene

melY

Organism
Enterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for transport of melibiose into the cell, with the concomitant import of a proton (symport system) (PubMed:9209070, PubMed:9375783, PubMed:12775706, PubMed:18177889). Can also transport lactose, and has weak activity with maltose (PubMed:18177889, PubMed:12775706). Cannot transport the analog methyl-1-thio-beta,D-galactopyranoside (TMG) (PubMed:18177889).4 Publications

Kineticsi

  1. KM=0.26 mM for melibiose1 Publication
  2. KM=0.51 mM for lactose1 Publication
  3. KM=14 mM for maltose1 Publication
  1. Vmax=82 nmol/min/mg enzyme with melibiose as substrate1 Publication
  2. Vmax=54 nmol/min/mg enzyme with lactose as substrate1 Publication
  3. Vmax=121 nmol/min/mg enzyme with maltose as substrate1 Publication

GO - Molecular functioni

Keywordsi

Biological processSugar transport, Transport

Enzyme and pathway databases

BioCyciECLO716541:GH13-5137-MONOMER

Protein family/group databases

TCDBi2.A.1.5.4 the major facilitator superfamily (mfs)
2.A.1.5.5 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Melibiose permease1 Publication
Alternative name(s):
Melibiose transporter MelYCurated
Gene namesi
Name:melY2 Publications
Ordered Locus Names:ECL_05077Imported
OrganismiEnterobacter cloacae subsp. cloacae (strain ATCC 13047 / DSM 30054 / NBRC 13535 / NCDC 279-56)
Taxonomic identifieri716541 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex
Proteomesi
  • UP000002363 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13Cytoplasmic1 PublicationAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 50Periplasmic1 PublicationAdd BLAST16
Transmembranei51 – 71HelicalSequence analysisAdd BLAST21
Topological domaini72 – 80Cytoplasmic1 Publication9
Transmembranei81 – 101HelicalSequence analysisAdd BLAST21
Topological domaini102 – 107Periplasmic1 Publication6
Transmembranei108 – 128HelicalSequence analysisAdd BLAST21
Topological domaini129 – 149Cytoplasmic1 PublicationAdd BLAST21
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171Periplasmic1 Publication1
Transmembranei172 – 192HelicalSequence analysisAdd BLAST21
Topological domaini193 – 227Cytoplasmic1 PublicationAdd BLAST35
Transmembranei228 – 248HelicalSequence analysisAdd BLAST21
Topological domaini249 – 267Periplasmic1 PublicationAdd BLAST19
Transmembranei268 – 288HelicalSequence analysisAdd BLAST21
Topological domaini289 – 297Cytoplasmic1 Publication9
Transmembranei298 – 318HelicalSequence analysisAdd BLAST21
Topological domaini319 – 325Periplasmic1 Publication7
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Topological domaini347 – 353Cytoplasmic1 Publication7
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21
Topological domaini375 – 385Periplasmic1 PublicationAdd BLAST11
Transmembranei386 – 406HelicalSequence analysisAdd BLAST21
Topological domaini407 – 425Cytoplasmic1 PublicationAdd BLAST19

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi88L → P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport. 1 Publication1
Mutagenesisi91L → P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport. 1 Publication1
Mutagenesisi182A → P: Does not affect melibiose transport. Increases affinity for maltose and maltose transport. 1 Publication1
Mutagenesisi367A → V: No change in transport activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004427421 – 425Melibiose permeaseAdd BLAST425

Structurei

3D structure databases

ProteinModelPortaliP96517
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105U0F Bacteria
ENOG410XQTD LUCA
KOiK02532
OMAiFIMATCF

Family and domain databases

CDDicd06174 MFS, 1 hit
InterProiView protein in InterPro
IPR000576 LacY/RafB_perm_fam
IPR018457 LacY/RafB_perm_fam_CS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
PfamiView protein in Pfam
PF01306 LacY_symp, 1 hit
PRINTSiPR00174 LACYSMPORT
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00882 2A0105, 1 hit
PROSITEiView protein in PROSITE
PS00896 LACY_1, 1 hit
PS00897 LACY_2, 1 hit
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

P96517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTTCTHKD NPNFWIFGLF FFLYFFIMAT CFPFLPIWLS DIIGLNKTHT
60 70 80 90 100
GIVFSCISLS AIAFQPVLGV ISDKLGLKKH LLWIISVLLF LFAPFFLYVF
110 120 130 140 150
APLLKTNIWL GALSGGLYIG FVFSAGSGAI EAYIERVSRN SAFEYGKARM
160 170 180 190 200
FGCLGWGLCA STGGILFGID PSYVFWMGSA AALLLMLLLV VAKPKPNQTA
210 220 230 240 250
QVMNALGANQ PQITAKKVFN LFRQRRMWMF ILYVIGVACV YDVFDQQFAT
260 270 280 290 300
FFKTFFATPQ EGTRAFGFAT TAGEICNAII MFCSPWIINR IGAKNTLLIA
310 320 330 340 350
GLIMATRIIG SSFATTAVEV IALKMLHALE VPFLLVGAFK YITGVFDTRL
360 370 380 390 400
SATIYLIGFQ FAKQSAAIFL SAFAGNMYDR IGFQETYLML GCFVLAITVV
410 420
SAFTLSSRQE IAAAAGAAAL TSQSR
Length:425
Mass (Da):46,701
Last modified:May 1, 1997 - v1
Checksum:iD49AF033F9105D51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000622 Genomic DNA Translation: BAA19154.1
CP001918 Genomic DNA Translation: ADF64599.1
RefSeqiWP_013099342.1, NC_014121.1
YP_003615548.1, NC_014121.1

Genome annotation databases

EnsemblBacteriaiADF64599; ADF64599; ECL_05077
GeneIDi9127542
KEGGienc:ECL_05077
PATRICifig|550.124.peg.580

Similar proteinsi

Entry informationi

Entry nameiMELY_ENTCC
AccessioniPrimary (citable) accession number: P96517
Secondary accession number(s): A0A0H3CTM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2017
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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