Reviewed, UniProtKB/Swiss-Prot P96513 (AMYB_BACFI)

Names and origin

Protein names	Recommended name:     Beta-amylase     EC=3.2.1.2 Alternative name(s):     1,4-alpha-D-glucan maltohydrolase
Organism	Bacillus firmus
Taxonomic identifier	1399 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	468 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
Sequence similarities	Belongs to the glycosyl hydrolase 14 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Polysaccharide degradation
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Potential
	Chain	37 – ›468	›432	Beta-amylase		PRO_0000001455
Sites
	Active site	198	1	By similarity
	Active site	394	1	By similarity
Amino acid modifications
	Disulfide bond	118 ↔ 126		By similarity
Experimental info
	Non-terminal residue	468	1

Sequences

	Sequence		Length	Mass (Da)
	P96513-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 4B0C11D7FD7316AF	FASTA	468	51,118
	10 20 30 40 50 60 MTLYRSLWKK GCMLLLSLVL SLTAFIGSPS NTASAAVADD IQASVMGPLA KINDWGSFKK 70 80 90 100 110 120 QLQTLKNNGV YAITTDVWWG YVESAGDNQF DWSYYKTYAD AVKEAGLKWV PIISTHKCGG 130 140 150 160 170 180 NVGDDCNIPL PSWLSSKGSA DEMQFKDESG YANNEALSPL WSGTGKQYDE LYASFAQNFA 190 200 210 220 230 240 GYKSIIPKIY LSGGPSGELR YPSYYPAAGW SYPGRGKFQA YTETAKNAFR TAMNDKYGSL 250 260 270 280 290 300 DKINTAWGTK LTSLSQINPP TDGDGFYTNG GYNSAYGKDF LSWYQSVLEK HLGVIGAAAH 310 320 330 340 350 360 KNFDSVFGVR IGAKISGLHW QMNNPAMPHS TEQAGGYYDY NRLIQKFKDA DLDLTFTCLE 370 380 390 400 410 420 MSDSGTAPNY SLPSTLVDTV SSIANAKGVR LNGENALQTG GSGFQKIEEK ITKFGYHGFT 430 440 450 460 LLRINNLVNN DGSPTGELSG FKQYIISKAK PDNNGGTGNK VTIYYKKG

References

[1]	"Sequencing of a beta-amylase gene from Bacillus firmus." Chen W., He B., Lou X., Guan F., Ye C. Wei Sheng Wu Xue Bao 38:142-145(1998) [PubMed: 12549376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 725.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB000264 Genomic DNA. Translation: BAA19075.1.
3D structure databases
SMR	P96513. Positions 35-466.
ModBase	Search...
Protein family/group databases
CAZy	CBM25. Carbohydrate-Binding Module Family 25. GH14. Glycoside Hydrolase Family 14.
Enzyme and pathway databases
BRENDA	3.2.1.2. 20639.
Family and domain databases
InterPro	IPR001554. Glyco_hydro_14. IPR018238. Glyco_hydro_14_CS. IPR000125. Glyco_hydro_14A_bac. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF01373. Glyco_hydro_14. 1 hit. [Graphical view]
PRINTS	PR00750. BETAAMYLASE. PR00841. GLHYDLASE14A.
PROSITE	PS00506. BETA_AMYLASE_1. 1 hit. PS00679. BETA_AMYLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYB_BACFI

Accession

Primary (citable) accession number: P96513

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)