P96465 (BLA2_STEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-lactamase L2 EC=3.5.2.6 Alternative name(s): Penicillinase
Organism	Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia)
Taxonomic identifier	40324 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Stenotrophomonas › Stenotrophomonas maltophilia group

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Tat-type signal Potential
	Chain	36 – 303	268	Beta-lactamase L2		PRO_0000017021
Regions
	Region	247 – 249	3	Substrate binding By similarity
Sites
	Active site	83	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96465 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: C278DAF4C824FD3C	FASTA	303	32,208
	10 20 30 40 50 60 MLARRRFLQF SGAAVASSLA LPLLARAAGK TAASAPTDAA LTAATDFAAL EKAVRGRFGV 70 80 90 100 110 120 TLLDTASGRR IGHRQDERFP MCSTFKSVLA ATVLSQAERQ PALLDTRVPV RDADLLSHAP 130 140 150 160 170 180 VTRRHAGKDM TVRDLCRATI ITSDNTAANL LFGVVGGPPA VTAFLRSIGD AVSRTDRLEP 190 200 210 220 230 240 ELNSFAKGDP RDTTTPAAMA ATLQRVVLGE VLQLASRQQL ADWLIDNETG DACLRAGLGK 250 260 270 280 290 300 LWRVRDKTGS NGEDARNDIA VLWPVAGGAP WVLTAYLQAG AISYEQRATV LAQVGRIADR LIG

References

[1]	"Sequence analysis and enzyme kinetics of the L2 serine beta-lactamase from Stenotrophomonas maltophilia." Walsh T.R., Macgowan A.P., Bennett P.M. Antimicrob. Agents Chemother. 41:1460-1464(1997) [PubMed: 9210666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IID 1275.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08562 Genomic DNA. Translation: CAA69869.1.
3D structure databases
ProteinModelPortal	P96465.
SMR	P96465. Positions 33-303.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. IPR000871. Beta-lactam_class-A/D. IPR023650. Beta-lactam_class-A_AS. IPR006311. TAT_signal. [Graphical view]
Gene3D	G3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA2_STEMA

Accession

Primary (citable) accession number: P96465

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 31, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program