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P96463

- EABF_STRLI

UniProt

P96463 - EABF_STRLI

Protein

Extracellular exo-alpha-L-arabinofuranosidase

Gene

abfB

Organism
Streptomyces lividans
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Involved in the degradation of xylan and is a key enzyme in the complete degradation of the plant cell wall. It has a specific arabinofuranose-debranching activity on xylan from gramineae. Acts synergistically with the xylanases and binds specifically to xylan. From small arabinoxylo-oligosides (ranging from arabinoxylotriose to arabinoxylohexaose), it liberates arabinose and, after prolonged incubation, the purified enzyme exhibits some xylanolytic activity as well.1 Publication

    Catalytic activityi

    Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

    Kineticsi

    1. KM=1.17 mM for wheat arabinoxylan (at 55 degrees Celsius)1 Publication
    2. KM=5.12 mM for oat xylan (at 55 degrees Celsius)1 Publication

    Vmax=17.2 µmol/min/mg enzyme with oat xylan as substrate (at 55 degrees Celsius)1 Publication

    Vmax=18.5 µmol/min/mg enzyme with wheat arabinoxylan as substrate (at 55 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 6.1 Publication

    Temperature dependencei

    Optimum temperature is 55 degrees Celsius.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. L-arabinose metabolic process Source: InterPro
    2. xylan catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Polysaccharide degradation, Xylan degradation

    Keywords - Ligandi

    Lectin

    Enzyme and pathway databases

    UniPathwayiUPA00114.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GH62. Glycoside Hydrolase Family 62.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Extracellular exo-alpha-L-arabinofuranosidase (EC:3.2.1.55)
    Short name:
    ABF
    Alternative name(s):
    Arabinosidase
    Arabinoxylan arabinofuranohydrolase
    Gene namesi
    Name:abfB
    OrganismiStreptomyces lividans
    Taxonomic identifieri1916 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    Subcellular locationi

    Secreted 1 Publication

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3737Sequence AnalysisAdd
    BLAST
    Chaini38 – 475438Extracellular exo-alpha-L-arabinofuranosidasePRO_0000008037Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP96463.
    SMRiP96463. Positions 38-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 166128Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 62 family.Curated
    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR005193. GH62_arabinosidase.
    IPR023296. Glyco_hydro_beta-prop.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF03664. Glyco_hydro_62. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF75005. SSF75005. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P96463-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHRGSLSRGQ HVRGTRRRGA ALAALAALLV ATAPAQAAGS GALRGAGSNR    50
    CLDVLGGSQD DGALLQLYDC WGGTNQQWTS TDTGRLTVYG DKCLDVPGHA 100
    TAPGTRVQIW SCSGGRNQQW RVNSDGTVVG VESGLCLEAA GAGTPNGTAV 150
    QLWTCNGGGN QKWTGLTGTP PTDGTCALPS TYRWSSTGVL AQPKSGWVAL 200
    KDFTTVTHNG RHLVYGSTSS GSSYGSMVFS PFTNWSDMAS AGQNAMNQAA 250
    VAPTLFYFAP KNIWVLAYQW GSWPFIYRTS SDPTDPNGWS APQPLFTGSI 300
    SGSDTGPIDQ TLIADGQNMY LFFAGDNGKI YRASMPIGNF PGNFGSSYTT 350
    IMSDTKANLF EGVQVYKVQG QNQYLMIVEA MGANGRYFRS FTASSLSGSW 400
    TPQAASEGNP FAGKANSGAT WTNDISHGDL VRDNPDQTMT VDPCNLQFLY 450
    QGKAPNAGGH YNSLPWRPGV LTLRH 475
    Length:475
    Mass (Da):50,369
    Last modified:December 15, 1998 - v2
    Checksum:iC3CB14EE7BF85AAD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64551 Genomic DNA. Translation: AAC26524.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64551 Genomic DNA. Translation: AAC26524.1 .

    3D structure databases

    ProteinModelPortali P96463.
    SMRi P96463. Positions 38-164.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GH62. Glycoside Hydrolase Family 62.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00114 .

    Family and domain databases

    InterProi IPR005193. GH62_arabinosidase.
    IPR023296. Glyco_hydro_beta-prop.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF03664. Glyco_hydro_62. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF75005. SSF75005. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "New alpha-L-arabinofuranosidase produced by Streptomyces lividans: cloning and DNA sequence of the abfB gene and characterization of the enzyme."
      Vincent P., Shareck F., Dupont C., Morosoli R., Kluepfel D.
      Biochem. J. 322:845-852(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, SUBSTRATE SPECIFICITY.
      Strain: 66 / 1326.
    2. Shareck F.
      Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.

    Entry informationi

    Entry nameiEABF_STRLI
    AccessioniPrimary (citable) accession number: P96463
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    These dual activities might be explained by a rotation about the alpha-(1->3)-glycosidic bond of the arabinofuranose group to the xylose moiety of xylan, which produces a bond conformation resembling that of a beta-(1->4) bond found in xylosides. Thus a single catalytic site could perform the double activity (PubMed:9148759).1 Publication

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3