P96426 (ARGJ_RHOFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine biosynthesis bifunctional protein ArgJ

Cleaved into the following 2 chains:

1. Arginine biosynthesis bifunctional protein ArgJ alpha chain
2. Arginine biosynthesis bifunctional protein ArgJ beta chain

Including the following 2 domains:

1. Glutamate N-acetyltransferase
   EC=2.3.1.35
   Alternative name(s):
   Ornithine acetyltransferase
   Short name=OATase
   Ornithine transacetylase
2. Amino-acid acetyltransferase
   EC=2.3.1.1
   Alternative name(s):
   N-acetylglutamate synthase
   Short name=AGSase

Gene names

Name:	argJ
Synonyms:	attH

Organism

Rhodococcus fascians

Taxonomic identifier

1828 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Rhodococcus

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate By similarity. HAMAP-Rule MF_01106
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP-Rule MF_01106 Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP-Rule MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP-Rule MF_01106 Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP-Rule MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Some bacteria possess a monofunctional ArgJ, i.e. capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP-Rule MF_01106
Sequence similarities	Belongs to the ArgJ family.
Sequence caution	The sequence CAC43342.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Autocatalytic cleavage
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Arginine biosynthesis bifunctional protein ArgJ alpha chain By similarity		PRO_0000002227
	Chain	194 – 403	210	Arginine biosynthesis bifunctional protein ArgJ beta chain By similarity		PRO_0000002228
Sites
	Active site	194	1	Nucleophile By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	273	1	Substrate By similarity
	Binding site	398	1	Substrate By similarity
	Binding site	403	1	Substrate By similarity
	Site	125	1	Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole By similarity
	Site	126	1	Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole By similarity
	Site	193 – 194	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96426 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: B596508AD60FD900	FASTA	403	42,800
	10 20 30 40 50 60 MVQSVLSSTS HGSERADMSA ASPQGFHCWT THLGLADEGK DDFSMVISDR PCTSSVVFTK 70 80 90 100 110 120 SLFAGPCVTL SKTSIEQTSP RGVLVLAKNA NVATGAEGLR NASEIRASVA RTVGIEPDAL 130 140 150 160 170 180 IMASTGVIGV QYPMDTIRAG LDGLGQGTPL DAHAVARAIM TTDTRAKVSE RAVGTSTIVG 190 200 210 220 230 240 IAKGVGMIEP DMATMLSFVF TDADVPQDTL NRIFRDVVDR TYNSVSIDTD TSTSDTAAVF 250 260 270 280 290 300 ANGSAGPVPD TEFEQALEEV CTDLVKMIAS DGEGATKLIV TTVSGASSER QARVVGKSII 310 320 330 340 350 360 NSPLVKTMIH GEDPNWGRVL MAIGKCSNEV DIEPDRIRVA FADIDVYPSS SLDQHDTVEV 370 380 390 400 VREHLATVTV EINVDLGIGT DTWRVFGCDL TDEYIRINAD YTT

References

[1]	"Leafy gall formation is controlled by fasR, an AraC-type regulatory gene in Rhodococcus fascians." Temmerman W., Vereecke D., Dreesen R., Van Montagu M., Holsters M., Goethals K. J. Bacteriol. 182:5832-5840(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D188.
[2]	"The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound." Maes T., Vereecke D., Ritsema T., Cornelis K., Thu H.N., Van Montagu M., Holsters M., Goethals K. Mol. Microbiol. 42:13-28(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: D188.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09820 Genomic DNA. Translation: CAA70949.1. AJ311775 Genomic DNA. Translation: CAC43342.1. Different initiation.
3D structure databases
ProteinModelPortal	P96426.
SMR	P96426. Positions 22-400.
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00068; UER00106. UPA00068; UER00111.
Family and domain databases
Gene3D	3.60.70.12. 1 hit.
HAMAP	MF_01106. ArgJ.
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. ArgJ-like_dom. [Graphical view]
PANTHER	PTHR23100. PTHR23100. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. Arg_biosynth_ArgJ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_RHOFA

Accession

Primary (citable) accession number: P96426
Secondary accession number(s): Q93JQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program