Reviewed, UniProtKB/Swiss-Prot P96417 (GABD2_MYCTU)

Names and origin

Protein names

Recommended name:
    Putative succinate-semialdehyde dehydrogenase [NADP+] 2
      Short name=SSADH 2
      Short name=SSDH 2
    EC=1.2.1.16

Gene names

Name:	gabD2
Ordered Locus Names:	Rv1731, MT1772

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the NADP+-dependent oxidation of succinate semialdehyde to succinate. Although it has succinate semialdehyde dehydrogenase activity, is likely to act physiologically on a different aldehyde(s). NAD+ can substitute for NADP+, but enzymatic activity is three times reduced. Ref.3
Catalytic activity	Succinate semialdehyde + NAD(P)+ + H2O = succinate + NAD(P)H. Ref.3
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinate-semialdehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Putative succinate-semialdehyde dehydrogenase [NADP+] 2		PRO_0000310711
Regions
	Nucleotide binding	232 – 237	6	NAD or NADP By similarity
Sites
	Active site	254	1	By similarity
	Active site	288	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96417-1 [UniParc]. Last modified July 5, 2004. Version 3. Checksum: E3AC9E895EB46270	FASTA	518	55,324
	10 20 30 40 50 60 MPAPSAEVFD RLRNLAAIKD VAARPTRTID EVFTGKPLTT IPVGTAADVE AAFAEARAAQ 70 80 90 100 110 120 TDWAKRPVIE RAAVIRRYRD LVIENREFLM DLLQAEAGKA RWAAQEEIVD LIANANYYAR 130 140 150 160 170 180 VCVDLLKPRK AQPLLPGIGK TTVCYQPKGV VGVISPWNYP MTLTVSDSVP ALVAGNAVVL 190 200 210 220 230 240 KPDSQTPYCA LACAELLYRA GLPRALYAIV PGPGSVVGTA ITDNCDYLMF TGSSATGSRL 250 260 270 280 290 300 AEHAGRRLIG FSAELGGKNP MIVARGANLD KVAKAATRAC FSNAGQLCIS IERIYVEKDI 310 320 330 340 350 360 AEEFTRKFGD AVRNMKLGTA YDFSVDMGSL ISEAQLKTVS GHVDDATAKG AKVIAGGKAR 370 380 390 400 410 420 PDIGPLFYEP TVLTNVAPEM ECAANETFGP VVSIYPVADV DEAVEKANDT DYGLNASVWA 430 440 450 460 470 480 GSTAEGQRIA ARLRSGTVNV DEGYAFAWGS LSAPMGGMGL SGVGRRHGPE GLLKYTESQT 490 500 510 IATARVFNLD PPFGIPATVW QKSLLPIVRT VMKLPGRR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: identification of alpha-ketoglutarate decarboxylase." Tian J., Bryk R., Itoh M., Suematsu M., Nathan C. Proc. Natl. Acad. Sci. U.S.A. 102:10670-10675(2005) [PubMed: 16027371] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY. Strain: ATCC 25618 / H37Rv.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX842577 Genomic DNA. Translation: CAB07016.2. AE000516 Genomic DNA. Translation: AAK46045.1.
PIR	H70962.
RefSeq	NP_214748.2. NP_336231.1.
3D structure databases
HSSP	HSSP built from PDB template 1AG8 based on UniProtKB P20000.
ModBase	Search...
Genome annotation databases
GeneID	885204. 923923.
GenomeReviews	Gene locus MT1772 in contig AE000516_GR. Gene locus Rv1731 in contig AL123456_GR.
KEGG	mtc:MT1772. mtu:Rv1731.
TIGR	MT1772.
Organism-specific databases
TubercuList	Rv1731.
Phylogenomic databases
HOGENOM	P96417.
OMA	P96417. DTEYGLN.
Enzyme and pathway databases
BRENDA	1.2.1.16. 809.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GABD2_MYCTU

Accession

Primary (citable) accession number: P96417
Secondary accession number(s): Q7D824

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 59 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)