Reviewed, UniProtKB/Swiss-Prot P96348 (BLA6_KLEPN)

Names and origin

Protein names

Recommended name:
    Beta-lactamase SHV-6
    EC=3.5.2.6

Gene names

Name:	bla
Synonyms:	shv6

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	260 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	SHV enzymes hydrolyze broad spectrum cephalosporins notably cefotaxime and ceftazidime.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 11	›11	Potential
	Chain	12 – ›260	›249	Beta-lactamase SHV-6		PRO_0000016985
Regions
	Region	220 – 222	3	Substrate binding By similarity
Sites
	Active site	56	1	Acyl-ester intermediate By similarity
	Active site	154	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	63 ↔ 109		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	260	1

Sequences

	Sequence		Length	Mass (Da)
	P96348-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 26A8E2D43632EAA0	FASTA	260	28,157
	10 20 30 40 50 60 LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE RFPMMSTFKV 70 80 90 100 110 120 VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA AAITMSDNSA 130 140 150 160 170 180 ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARATTTPA SMAATLRKLL 190 200 210 220 230 240 TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG IVALLGPNNK 250 260 AERIVVIYLR DTPASMAERN

References

[1]	"Substitution of alanine for aspartate at position 179 in the SHV-6 extended-spectrum beta-lactamase." Arlet G., Rouveau M., Philippon A. FEMS Microbiol. Lett. 152:163-167(1997) [PubMed: 9228783] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SLK-47.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11069 Genomic DNA. Translation: CAA71948.1.
3D structure databases
HSSP	HSSP built from PDB template 1SHV based on UniProtKB P14557.
SMR	P96348. Positions 12-260.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 758.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA6_KLEPN

Accession

Primary (citable) accession number: P96348

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)