Reviewed, UniProtKB/Swiss-Prot P96318 (IDH_CALNO)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase [NADP]
      Short name=IDH
    EC=1.1.1.42
Alternative name(s):
    Oxalosuccinate decarboxylase
    NADP(+)-specific ICDH
    IDP

Gene names

Name:

icd

Organism

Caldococcus noboribetus

Taxonomic identifier

57174 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Desulfurococcales › Caldococcus

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 98 degrees Celsius. Thermostable.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Isocitrate dehydrogenase [NADP]		PRO_0000083573
Regions
	Nucleotide binding	340 – 346	7	NADP By similarity
Sites
	Metal binding	308	1	Magnesium or manganese By similarity
	Binding site	108	1	NADP By similarity
	Binding site	117	1	Substrate By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	123	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	353	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	393	1	NADP By similarity
	Binding site	397	1	NADP By similarity
	Site	163	1	Critical for catalysis By similarity
	Site	230	1	Critical for catalysis By similarity
Amino acid modifications
	Modified residue	117	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96318-1 [UniParc]. Last modified May 1, 2000. Version 2. Checksum: F06A559FCAA1C42B	FASTA	429	47,620
	10 20 30 40 50 60 MVSHPCTADE AKPPSEGQLA RFENGKLIVP DNLIVAYFKG DGIGPEIVES AKKVLDAAVD 70 80 90 100 110 120 KAYGGTRRIV WWEVTAGEEA QKECGSLLPD GTLQAFKLAR VNLKGPLTTP VGGGFRSLNV 130 140 150 160 170 180 TLRMVLDLYS NVRPVKWYGQ PTPHCHPENI DWVIFRENTE DVYAGIEWPF DSPEAQKIRD 190 200 210 220 230 240 FLKKEFGIEL TPDTGIGIKP ISKWRTQRHV RRAMEWAIRN GYKHVTIMHK GNIMKYTEGA 250 260 270 280 290 300 FRQWAYDLIL SEFRDYVVTE EEVNTKYGGK APEGKIIVND RIADNMLQQI ITRPGEYNVI 310 320 330 340 350 360 VTPNLNGDYI SDEANALVGG IGMAAGLDMG DGIAVAEPVH GSAPKYAGKN VINPTAEILS 370 380 390 400 410 420 GMYLLSDFVG WPEVKLLVEY AVKQAIAHKQ VTYDLAREMG GVTPISTTEY TDVLVDYIRH ADLKALKGQ

References

[1]

"Eubacteria-type isocitrate dehydrogenase from an Archaeon: cloning, sequencing, and expression of a gene encoding isocitrate dehydrogenase from a hyperthermophilic archaebacterium Caldococcus noboribetus." Aoshima M., Yamagishi A., Oshima T. Arch. Biochem. Biophys. 336:77-85(1996) [PubMed: 8951037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NC12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86855 Genomic DNA. Translation: BAA13177.2.
PIR	T44658.
3D structure databases
HSSP	HSSP built from PDB template 1HQS based on UniProtKB P39126.
ModBase	Search...
Family and domain databases
InterPro	IPR004439. IsoCit_DHase_NADP_prok. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF1. NADP_IDH_prok. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00183. prok_nadp_idh. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDH_CALNO

Accession

Primary (citable) accession number: P96318

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 2000
Last modified:	November 25, 2008
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)