P96282 (PSS_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CDP-diacylglycerol--serine O-phosphatidyltransferase
EC=2.7.8.8

Alternative name(s):
Phosphatidylserine synthase

Gene names

Name:	pssA
Ordered Locus Names:	Rv0436c, MT0452
ORF Names:	MTCY22G10.33c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	CDP-diacylglycerol + L-serine = CMP + (3-sn-phosphatidyl)-L-serine.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
Sequence caution	The sequence AAK44675.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	CDP-diacylglycerol metabolic process Inferred from electronic annotation. Source: InterPro phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from direct assay. Source: MTBBASE integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CDP-diacylglycerol-serine O-phosphatidyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	CDP-diacylglycerol--serine O-phosphatidyltransferase		PRO_0000056798
Regions
	Transmembrane	15 – 35	21	Helical; Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	135 – 155	21	Helical; Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Transmembrane	207 – 227	21	Helical; Potential
Experimental info
	Sequence conflict	167	1	G → V in AAK44675. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P96282 [UniParc]. Last modified August 1, 1998. Version 2. Checksum: 17C46D9ADA650F50	FASTA	286	31,218
	10 20 30 40 50 60 MIGKPRGRRG VNLQILPSAM TVLSICAGLT AIKFALEHQP KAAMALIAAA AILDGLDGRV 70 80 90 100 110 120 ARILDAQSRM GAEIDSLADA VNFGVTPALV LYVSMLSKWP VGWVVVLLYA VCVVLRLARY 130 140 150 160 170 180 NALQDDGTQP AYAHEFFVGM PAPAGAVSMI GLLALKMQFG EGWWTSGWFL SFWVTGTSIL 190 200 210 220 230 240 LVSGIPMKKM HAVSVPPNYA AALLAVLAIC AAAAVLAPYL LIWVIIIAYM CHIPFAVRSQ 250 260 270 280 RWLAQHPEVW DDKPKQRRAV RRASRRAHPY RPSMARLGLR KPGRRL

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAB06576.1. AE000516 Genomic DNA. Translation: AAK44675.1. Different initiation.
PIR	B70632.
RefSeq	NP_214950.1. NC_000962.2. NP_334861.1. NC_002755.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000003528; EBMYCP00000003528; EBMYCG00000003526. EBMYCT00000069577; EBMYCP00000067636; EBMYCG00000069572.
GeneID	886385. 923770.
GenomeReviews	Gene locus MT0452 in contig AE000516_GR. Gene locus Rv0436c in contig AL123456_GR.
KEGG	mtc:MT0452. mtu:Rv0436c.
PATRIC	18122704. VBIMycTub22151_0486.
TIGR	MT0452.
Organism-specific databases
TubercuList	Rv0436c.
Phylogenomic databases
GeneTree	EBGT00050000015994.
HOGENOM	HBG735258.
OMA	CMSIRLA.
PhylomeDB	P96282.
ProtClustDB	CLSK790520.
Family and domain databases
InterPro	IPR004533. CDP-diaglyc--ser_O-PTrfase. IPR000462. CDP-OH_P_trans. [Graphical view]
KO	K00998.
Pfam	PF01066. CDP-OH_P_transf. 1 hit. [Graphical view]
TIGRFAMs	TIGR00473. PssA. 1 hit.
PROSITE	PS00379. CDP_ALCOHOL_P_TRANSF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSS_MYCTU

Accession

Primary (citable) accession number: P96282

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program