Reviewed, UniProtKB/Swiss-Prot P96254 (PTA_MYCTU)

Names and origin

Protein names

Recommended name:
    Phosphate acetyltransferase
    EC=2.3.1.8
Alternative name(s):
    Phosphotransacetylase

Gene names

Name:	pta
Ordered Locus Names:	Rv0408, MT0421
ORF Names:	MTCY22G10.04

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + phosphate = CoA + acetyl phosphate.
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.
Subcellular location	Cytoplasm Potential.
Sequence similarities	In the N-terminal section; belongs to the cobB/cobQ family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphate acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Phosphate acetyltransferase		PRO_0000179135
Regions
	Region	365 – 690	326	Phosphate acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P96254-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: C01C412AF2810CCE	FASTA	690	72,949
	10 20 30 40 50 60 MADSSAIYLA APESQTGKST IALGLLHRLT AMVAKVGVFR PITRLSAERD YILELLLAHT 70 80 90 100 110 120 SAGLPYERCV GVTYQQLHAD RDDAIAEIVD SYHAMADECD AVVVVGSDYT DVTSPTELSV 130 140 150 160 170 180 NGRIAVNLGA PVLLTVRAKD RTPDQVASVV EVCLAELDTQ RAHTAAVVAN RCELSAIPAV 190 200 210 220 230 240 TDALRRFTPP SYVVPEEPLL SAPTVAELTQ AVNGAVVSGD VALREREVMG VLAAGMTADH 250 260 270 280 290 300 VLERLTDGMA VITPGDRSDV VLAVASAHAA EGFPSLSCIV LNGGFQLHPA IAALVSGLRL 310 320 330 340 350 360 RLPVIATALG TYDTASAAAS ARGLVTATSQ RKIDTALELM DRHVDVAGLL AQLTIPIPTV 370 380 390 400 410 420 TTPQMFTYRL LQQARSDLMR IVLPEGDDDR ILKSAGRLLQ RGIVDLTILG DEAKVRLRAA 430 440 450 460 470 480 ELGVDLDGAT VIEPCASELH DQFADQYAQL RKAKGITVEH AREIMNDATY FGTMLVHNCH 490 500 510 520 530 540 ADGMVSGAAH TTAHTVRPAL EIIKTVPGIS TVSSIFLMCL PDRVLAYGDC AIIPNPTVEQ 550 560 570 580 590 600 LADIAICSAR TAAQFGIEPR VAMLSYSTGD SGKGADVDKV RAATELVRAR EPQLPVEGPI 610 620 630 640 650 660 QYDAAVEPSV AATKLRDSPV AGRATVLIFP DLNTGNNTYK AVQRSAGAIA IGPVLQGLRK 670 680 690 PVNDLSRGAL VDDIVNTVAI TAIQAQGVHE

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAB06578.1. AE000516 Genomic DNA. Translation: AAK44645.1.
PIR	F70628.
RefSeq	NP_214922.1. NP_334831.1.
3D structure databases
SMR	P96254. Positions 5-213, 204-323, 369-687.
ModBase	Search...
Genome annotation databases
GeneID	886401. 923688.
GenomeReviews	Gene locus MT0421 in contig AE000516_GR.
KEGG	mtc:MT0421. mtu:Rv0408.
TIGR	MT0421.
Organism-specific databases
TubercuList	Rv0408.
Phylogenomic databases
HOGENOM	HBG576804.
OMA	QFGVEPR.
Enzyme and pathway databases
BRENDA	2.3.1.8. 809.
Family and domain databases
InterPro	IPR010766. DRTGG. IPR016475. P-Actrans_bac. IPR004614. P_AcTrfase. IPR002505. PTA_PTB. [Graphical view]
Pfam	PF07085. DRTGG. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF006107. PhpActrans_proteobac. 1 hit.
TIGRFAMs	TIGR00651. pta. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTA_MYCTU

Accession

Primary (citable) accession number: P96254

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)