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Protein

Glutamate synthase [NADPH] large chain

Gene

gltB

Organism
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activity is stimulated by unphosphorylated GarA.1 Publication

Pathwayi: L-glutamate biosynthesis via GLT pathway

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route).
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamate synthase [NADPH] small chain (gltD), Glutamate synthase [NADPH] large chain (gltB)
This subpathway is part of the pathway L-glutamate biosynthesis via GLT pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate from 2-oxoglutarate and L-glutamine (NADP(+) route), the pathway L-glutamate biosynthesis via GLT pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei18For GATase activityBy similarity1
Metal bindingi1136Iron-sulfur (3Fe-4S)By similarity1
Metal bindingi1142Iron-sulfur (3Fe-4S)By similarity1
Metal bindingi1147Iron-sulfur (3Fe-4S)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1083 – 1135FMNBy similarityAdd BLAST53

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Glutamate biosynthesis

Keywords - Ligandi

3Fe-4S, FAD, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMTBH37RV:G185E-8157-MONOMER.
UniPathwayiUPA00634; UER00689.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate synthase [NADPH] large chain (EC:1.4.1.13)
Alternative name(s):
Glutamate synthase subunit alpha
Short name:
GLTS alpha chain
Gene namesi
Name:gltB
Ordered Locus Names:Rv3859c
OrganismiMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Taxonomic identifieri83332 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001584 Componenti: Chromosome

Organism-specific databases

TubercuListiRv3859c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MTBBASE
  • plasma membrane Source: MTBBASE
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004195451 – 1527Glutamate synthase [NADPH] large chainAdd BLAST1527

Proteomic databases

PaxDbiP96218.

Interactioni

Subunit structurei

Interacts with unphosphorylated GarA.1 Publication

Protein-protein interaction databases

IntActiP96218. 1 interactor.
STRINGi83332.Rv3859c.

Structurei

3D structure databases

ProteinModelPortaliP96218.
SMRiP96218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 416Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST399

Sequence similaritiesi

Belongs to the glutamate synthase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CBC. Bacteria.
COG0067. LUCA.
COG0069. LUCA.
COG0070. LUCA.
HOGENOMiHOG000031559.
InParanoidiP96218.
KOiK00265.
OMAiEHMWTPD.
PhylomeDBiP96218.

Family and domain databases

CDDicd02808. GltS_FMN. 1 hit.
Gene3Di2.160.20.60. 1 hit.
3.20.20.70. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017932. GATase_2_dom.
IPR002489. Glu_synth_asu_C.
IPR006982. Glu_synth_centr_N.
IPR002932. Glu_synthdom.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF04898. Glu_syn_central. 1 hit.
PF01645. Glu_synthase. 1 hit.
PF01493. GXGXG. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
SSF69336. SSF69336. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPKRVGLYN PAFEHDSCGV AMVVDMHGRR SRDIVDKAIT ALLNLEHRGA
60 70 80 90 100
QGAEPRSGDG AGILIQVPDE FLREAVDFEL PAPGSYATGI AFLPQSSKDA
110 120 130 140 150
AAACAAVQKI AEAEGLQVLG WRSVPTDDSS LGALSRDAMP TFRQVFLAGA
160 170 180 190 200
SGMALERRCY VVRKRAEHEL GTKGPGQDGP GRETVYFPSL SGQTLVYKGM
210 220 230 240 250
LTTPQLKAFY LDLQDERLTS ALGIVHSRFS TNTFPSWPLA HPFRRIAHNG
260 270 280 290 300
EINTVTGNEN WMRAREALIK TDIFGSAADV EKLFPICTPG ASDTARFDEV
310 320 330 340 350
LELLHLGGRS LAHAVLMMIP EAWERHESMD PARRAFYQYH ASLMEPWDGP
360 370 380 390 400
ASMTFTDGTV VGAVLDRNGL RPSRIWVTDD GLVVMASEAG VLDLHPSTVV
410 420 430 440 450
RRMRLQPGRM FLVDTAQGRI VSDEEIKADL AAEHPYQEWL DNGLVPLDEL
460 470 480 490 500
PEGKDVRMPH HRIVMRQLAF GYTYEELNLL VAPMARLGAE PIGSMGTDTP
510 520 530 540 550
VAVLSQRPRM LYDYFHQLFA QVTNPPLDAI REEVVTSLQG TTGGERDLLN
560 570 580 590 600
PDQNSCHQIV LPQPILRNHE LAKLVSLDPN DKVNGRPHGL RSKVIRCLYR
610 620 630 640 650
VSEGGAGLAA ALEEVRGAAA AAIADGARII ILSDRESDEE MAPIPSLLAV
660 670 680 690 700
AGVHHHLVRE RTRTQVGLVV ESGDAREVHH MAALVGFGAA AINPYLVFES
710 720 730 740 750
IEDMLDRGVI EGIDRTAALN NYIKAAGKGV LKVMSKMGIS TLASYTGAQL
760 770 780 790 800
FQAVGISEQV LDEYFTGLTC PTGGITLDDI AADVAARHRL AYLDRPDERA
810 820 830 840 850
HRELEVGGEY QWRREGEYHL FNPETVFKLQ HSTRTGQYKI FKEYTRLVDD
860 870 880 890 900
QSERMASLRG LLKFRTGVRP PVPLDEVEPA SEIVKRFSTG AMSYGSISAE
910 920 930 940 950
AHETLAIAMN RLGARSNCGE GGEDVKRFDR DPNGDWRRSA IKQVASARFG
960 970 980 990 1000
VTSHYLTNCT DLQIKMAQGA KPGEGGQLPG HKVYPWVAEV RHSTPGVGLI
1010 1020 1030 1040 1050
SPPPHHDIYS IEDLAQLIHD LKNANPSARV HVKLVSENGV GTVAAGVSKA
1060 1070 1080 1090 1100
HADVVLISGH DGGTGATPLT SMKHAGAPWE LGLAETQQTL LLNGLRDRIV
1110 1120 1130 1140 1150
VQVDGQLKTG RDVMIATLLG AEEFGFATAP LVVAGCIMMR VCHLDTCPVG
1160 1170 1180 1190 1200
VATQNPLLRE RFTGKPEFVE NFFMFIAEEV REYLAQLGFR TVNEAVGQAG
1210 1220 1230 1240 1250
ALDTTLARAH WKAHKLDLAP VLHEPESAFM NQDLYCSSRQ DHGLDKALDQ
1260 1270 1280 1290 1300
QLIVMSREAL DSGKPVRFST TIGNVNRTVG TMLGHELTKA YGGQGLPDGT
1310 1320 1330 1340 1350
IDITFDGSAG NSFGAFVPKG ITLRVYGDAN DYVGKGLSGG RIVVRPSDDA
1360 1370 1380 1390 1400
PQDYVAEDNI IGGNVILFGA TSGEVYLRGV VGERFAVRNS GAHAVVEGVG
1410 1420 1430 1440 1450
DHGCEYMTGG RVVILGRTGR NFAAGMSGGV AYVYDPDGEL PANLNSEMVE
1460 1470 1480 1490 1500
LETLDEDDAD WLHGTIQVHV DATDSAVGQR ILSDWSGQQR HFVKVMPRDY
1510 1520
KRVLQAIALA ERDGVDVDKA IMAAAHG
Length:1,527
Mass (Da):165,943
Last modified:July 5, 2004 - v3
Checksum:i7ADAB46904A3AE46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46688.1.
PIRiH70655.
RefSeqiNP_218376.1. NC_000962.3.
WP_003899733.1. NZ_KK339374.1.

Genome annotation databases

EnsemblBacteriaiCCP46688; CCP46688; Rv3859c.
GeneIDi886195.
KEGGimtu:Rv3859c.
PATRICi18157202. VBIMycTub87468_4303.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL123456 Genomic DNA. Translation: CCP46688.1.
PIRiH70655.
RefSeqiNP_218376.1. NC_000962.3.
WP_003899733.1. NZ_KK339374.1.

3D structure databases

ProteinModelPortaliP96218.
SMRiP96218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP96218. 1 interactor.
STRINGi83332.Rv3859c.

Proteomic databases

PaxDbiP96218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCCP46688; CCP46688; Rv3859c.
GeneIDi886195.
KEGGimtu:Rv3859c.
PATRICi18157202. VBIMycTub87468_4303.

Organism-specific databases

TubercuListiRv3859c.

Phylogenomic databases

eggNOGiENOG4105CBC. Bacteria.
COG0067. LUCA.
COG0069. LUCA.
COG0070. LUCA.
HOGENOMiHOG000031559.
InParanoidiP96218.
KOiK00265.
OMAiEHMWTPD.
PhylomeDBiP96218.

Enzyme and pathway databases

UniPathwayiUPA00634; UER00689.
BioCyciMTBH37RV:G185E-8157-MONOMER.

Family and domain databases

CDDicd02808. GltS_FMN. 1 hit.
Gene3Di2.160.20.60. 1 hit.
3.20.20.70. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017932. GATase_2_dom.
IPR002489. Glu_synth_asu_C.
IPR006982. Glu_synth_centr_N.
IPR002932. Glu_synthdom.
IPR029055. Ntn_hydrolases_N.
[Graphical view]
PfamiPF00310. GATase_2. 1 hit.
PF04898. Glu_syn_central. 1 hit.
PF01645. Glu_synthase. 1 hit.
PF01493. GXGXG. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
SSF69336. SSF69336. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLTB_MYCTU
AccessioniPrimary (citable) accession number: P96218
Secondary accession number(s): F2GDM1, L0TDS2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Was identified as a high-confidence drug target.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh
    Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.