Reviewed, UniProtKB/Swiss-Prot P96198 (DHAS_AZOVI)

Names and origin

Protein names

Recommended name:
    Aspartate-semialdehyde dehydrogenase
      Short name=ASA dehydrogenase
      Short name=ASADH
    EC=1.2.1.11

Gene names

Name:

asd

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + phosphate + NADP+ = L-4-aspartyl phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 2/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aspartate-semialdehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro aspartate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Aspartate-semialdehyde dehydrogenase		PRO_0000141360
Sites
	Active site	134	1	Acyl-thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96198-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0F9B62D790C0AAD5	FASTA	368	40,388
	10 20 30 40 50 60 MKRVGLIGWR GMVGSVLMQR MLEERDFDLI EPVFFTTSSV GGQGAIGKET VPLKDAYSIE 70 80 90 100 110 120 ELKSLDAIIT CQGGDYTSEV FPKLRDAGWQ GYWIDAASSL RMADDAVIVL DPVNRRVIDQ 130 140 150 160 170 180 SLDAGVKNYI GGNCTVSLML MALGRLRGRP GRLDERHDLS GRFRAGAQNM RELIRQMGTI 190 200 210 220 230 240 NAAVAAEPAD PASAILEIDR KGPETQRSAE FPVDHFGVPL AGSLIPYIDK ELPNGQSREE 250 260 270 280 290 300 WKGQAETNKI LGRIKNPIPV DGICVRVGAM RCHSQALTIK LNKDVPIADI EGLISQHNPW 310 320 330 340 350 360 VKLVPNHREA SMQELSPAAI TGTLTVPVGR LRKLNMGSQY LGAFTVGDQL LWGAAEPVRR MLLILLER

References

[1]

"Characterization and mutagenesis of the leucine biosynthetic genes of Azotobacter vinelandii: an analysis of the rarity of amino acid auxotrophs." Manna A.C., Das H.K. Mol. Gen. Genet. 254:207-217(1997) [PubMed: 9108283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y11280 Genomic DNA. Translation: CAA72152.1.
3D structure databases
HSSP	HSSP built from PDB template 1BRM based on UniProtKB P00353.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.11. 883.
Family and domain databases
InterPro	IPR000319. Asp-semialdehyde_DH_CS. IPR011534. Asp_ADH_proteob. IPR012080. Asp_semialdehyde_DH. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
PIRSF	PIRSF000148. ASA_dh. 1 hit.
TIGRFAMs	TIGR01745. asd_gamma. 1 hit.
PROSITE	PS01103. ASD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHAS_AZOVI

Accession

Primary (citable) accession number: P96198

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)