Reviewed,
UniProtKB/Swiss-Prot P96197 (LEU3_AZOVI)
Last modified
February 9, 2010.
Version 61.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: 3-isopropylmalate dehydrogenase EC=1.1.1.85 Alternative name(s): Beta-IPM dehydrogenase Short name=IMDH 3-IPM-DH | ||
| Gene names |
| ||
| Organism | Azotobacter vinelandii | ||
| Taxonomic identifier | 354 [NCBI] | ||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter |
Protein attributes
| Sequence length | 360 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033 |
| Catalytic activity | (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033 |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_01033 |
| Pathway | Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033 |
| Subunit structure | Homodimer By similarity. HAMAP MF_01033 |
| Subcellular location | |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis |
| Cellular component | Cytoplasm |
| Ligand | Magnesium Manganese Metal-binding NAD |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | 3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH bindingInferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 360 | 360 | 3-isopropylmalate dehydrogenase HAMAP MF_01033 | PRO_0000083631 | |||||
Sites | |||||||||
| Metal binding | 223 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 247 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 251 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 133 | 1 | Substrate By similarity | ||||||
| Binding site | 223 | 1 | Substrate By similarity | ||||||
| Site | 140 | 1 | Important for catalysis By similarity | ||||||
| Site | 191 | 1 | Important for catalysis By similarity | ||||||
Sequences
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References
| [1] | "Characterization and mutagenesis of the leucine biosynthetic genes of Azotobacter vinelandii: an analysis of the rarity of amino acid auxotrophs." Manna A.C., Das H.K. Mol. Gen. Genet. 254:207-217(1997) [PubMed: 9108283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Y11280 Genomic DNA. Translation: CAA72151.1. |
3D structure databases | |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 1.1.1.85. 883. |
Family and domain databases | |
| HAMAP | MF_01033. LeuB_type1. [Tree] |
| InterPro | IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit. |
| Pfam | PF00180. Iso_dh. 2 hits. [Graphical view] |
| TIGRFAMs | TIGR00169. leuB. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LEU3_AZOVI | ||||||||
| Accession | Primary (citable) accession number: P96197 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


