Reviewed, UniProtKB/Swiss-Prot P96197 (LEU3_AZOVI)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:

leuB

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_01033
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033
Subunit structure	Homodimer By similarity. HAMAP MF_01033
Subcellular location	Cytoplasm HAMAP MF_01033.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	3-isopropylmalate dehydrogenase HAMAP MF_01033		PRO_0000083631
Sites
	Metal binding	223	1	Magnesium or manganese By similarity
	Metal binding	247	1	Magnesium or manganese By similarity
	Metal binding	251	1	Magnesium or manganese By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity
	Site	140	1	Important for catalysis By similarity
	Site	191	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96197-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 16AAEC7966EDAC6A	FASTA	360	38,974
	10 20 30 40 50 60 MSKQILVLPG DGIGPEIMAE AVKVLQLANE KYQLGFVLGY DELGGAAVDK YGVPLADETL 70 80 90 100 110 120 ERARRRCHPA RRRRRSEMGR HRPGHPPGAR PAENPFATGP VRQPAPGVLY PQLAEASTLR 130 140 150 160 170 180 PEVVAGLDIL IVRELTGGIY FGAPRESRLL ANGERMAYDT LPYSESEIRR IAKVGFDMAR 190 200 210 220 230 240 VRGKKLCSVD KANVLASSQL WRAVVEEVAK DYPDVVLSHM YVDNAAMQLV RAPKQFDVIV 250 260 270 280 290 300 TDNMFGDILS DEASMLTGSI GMLPSASLDA DNKGMYEPCH GSRRPDIAGK GIANPLATIL 310 320 330 340 350 360 SVSMMLRYSF GQVEAANAIE QAVSKVLDQG LRTGDIWSEG CRKVGTAEMG DAVVAALATL

References

[1]

"Characterization and mutagenesis of the leucine biosynthetic genes of Azotobacter vinelandii: an analysis of the rarity of amino acid auxotrophs." Manna A.C., Das H.K. Mol. Gen. Genet. 254:207-217(1997) [PubMed: 9108283] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11280 Genomic DNA. Translation: CAA72151.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.85. 883.
Family and domain databases
HAMAP	MF_01033. LeuB_type1. [Tree]
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 2 hits. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_AZOVI

Accession

Primary (citable) accession number: P96197

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)