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P96189

- T2X1_XANCR

UniProt

P96189 - T2X1_XANCR

Protein

Type-2 restriction enzyme XamI

Gene

xamIR

Organism
Xanthomonas campestris pv. amaranthicola
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 49 (16 Apr 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence GTCGAC and cleaves after G-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi2125. XamI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme XamI (EC:3.1.21.4)
    Short name:
    R.XamI
    Alternative name(s):
    Endonuclease XamI
    Type II restriction enzyme XamI
    Gene namesi
    Name:xamIR
    OrganismiXanthomonas campestris pv. amaranthicola
    Taxonomic identifieri54735 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 274274Type-2 restriction enzyme XamIPRO_0000077373Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019072. Restrct_endonuc_II_XamI.
    [Graphical view]
    PfamiPF09572. RE_XamI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P96189-1 [UniParc]FASTAAdd to Basket

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    MTSAPPRWTT AELAEDAATS AAQFRTERLA VTDSWGTHYN QARGKFELLF    50
    KKLSDLNPEA ITDDNLAEAY GLGLGEALRY LAGPPISDDD LQVIADVESI 100
    APGVLKKNAE ALRKVFGVIE RVIDPHRFPW METGGMPTEQ QREAALLASS 150
    VLLAAQRIAT ERRNEGKDNQ ETMVKDYLRT LGFTEAPAAA INTIVKGPQA 200
    MQFCAECQLG ERKADVVVRL HDTRLMAIEC KVSNSSTNSV KRLNNDAAVK 250
    AEYWIKQFGT AQVVPSRSLV SSRY 274
    Length:274
    Mass (Da):30,170
    Last modified:May 1, 1997 - v1
    Checksum:iF7617A101DC198AC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77781 Genomic DNA. Translation: AAD13687.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U77781 Genomic DNA. Translation: AAD13687.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 2125. XamI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019072. Restrct_endonuc_II_XamI.
    [Graphical view ]
    Pfami PF09572. RE_XamI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and nucleotide sequence of the gene encoding the XamI DNA methyltransferase of Xanthomonas campestris pv. amaranthicola."
      Gomez P., Ribas-Aparicio R.M., Pelaez A.I., Gomez A., Rodicio M.R.
      Biochim. Biophys. Acta 1351:261-266(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 11645 / LMG 498 / NCPPB 570.

    Entry informationi

    Entry nameiT2X1_XANCR
    AccessioniPrimary (citable) accession number: P96189
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 8, 2000
    Last sequence update: May 1, 1997
    Last modified: April 16, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3