Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type-2 restriction enzyme XamI

Gene

xamIR

Organism
Xanthomonas campestris pv. amaranthicola
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence GTCGAC and cleaves after G-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2125. XamI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme XamI (EC:3.1.21.4)
Short name:
R.XamI
Alternative name(s):
Endonuclease XamI
Type II restriction enzyme XamI
Gene namesi
Name:xamIR
OrganismiXanthomonas campestris pv. amaranthicola
Taxonomic identifieri54735 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 274274Type-2 restriction enzyme XamIPRO_0000077373Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019072. Restrct_endonuc_II_XamI.
[Graphical view]
PfamiPF09572. RE_XamI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96189-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSAPPRWTT AELAEDAATS AAQFRTERLA VTDSWGTHYN QARGKFELLF
60 70 80 90 100
KKLSDLNPEA ITDDNLAEAY GLGLGEALRY LAGPPISDDD LQVIADVESI
110 120 130 140 150
APGVLKKNAE ALRKVFGVIE RVIDPHRFPW METGGMPTEQ QREAALLASS
160 170 180 190 200
VLLAAQRIAT ERRNEGKDNQ ETMVKDYLRT LGFTEAPAAA INTIVKGPQA
210 220 230 240 250
MQFCAECQLG ERKADVVVRL HDTRLMAIEC KVSNSSTNSV KRLNNDAAVK
260 270
AEYWIKQFGT AQVVPSRSLV SSRY
Length:274
Mass (Da):30,170
Last modified:May 1, 1997 - v1
Checksum:iF7617A101DC198AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77781 Genomic DNA. Translation: AAD13687.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U77781 Genomic DNA. Translation: AAD13687.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2125. XamI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019072. Restrct_endonuc_II_XamI.
[Graphical view]
PfamiPF09572. RE_XamI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and nucleotide sequence of the gene encoding the XamI DNA methyltransferase of Xanthomonas campestris pv. amaranthicola."
    Gomez P., Ribas-Aparicio R.M., Pelaez A.I., Gomez A., Rodicio M.R.
    Biochim. Biophys. Acta 1351:261-266(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 11645 / LMG 498 / NCPPB 570.

Entry informationi

Entry nameiT2X1_XANCR
AccessioniPrimary (citable) accession number: P96189
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.