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P96189 (T2X1_XANCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme XamI

Short name=R.XamI
EC=3.1.21.4
Alternative name(s):
Endonuclease XamI
Type II restriction enzyme XamI
Gene names
Name:xamIR
OrganismXanthomonas campestris pv. amaranthicola
Taxonomic identifier54735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence GTCGAC and cleaves after G-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Type-2 restriction enzyme XamI
PRO_0000077373

Sequences

Sequence LengthMass (Da)Tools
P96189 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: F7617A101DC198AC

FASTA27430,170
        10         20         30         40         50         60 
MTSAPPRWTT AELAEDAATS AAQFRTERLA VTDSWGTHYN QARGKFELLF KKLSDLNPEA 

        70         80         90        100        110        120 
ITDDNLAEAY GLGLGEALRY LAGPPISDDD LQVIADVESI APGVLKKNAE ALRKVFGVIE 

       130        140        150        160        170        180 
RVIDPHRFPW METGGMPTEQ QREAALLASS VLLAAQRIAT ERRNEGKDNQ ETMVKDYLRT 

       190        200        210        220        230        240 
LGFTEAPAAA INTIVKGPQA MQFCAECQLG ERKADVVVRL HDTRLMAIEC KVSNSSTNSV 

       250        260        270 
KRLNNDAAVK AEYWIKQFGT AQVVPSRSLV SSRY 

« Hide

References

[1]"Isolation and nucleotide sequence of the gene encoding the XamI DNA methyltransferase of Xanthomonas campestris pv. amaranthicola."
Gomez P., Ribas-Aparicio R.M., Pelaez A.I., Gomez A., Rodicio M.R.
Biochim. Biophys. Acta 1351:261-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11645 / LMG 498 / NCPPB 570.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77781 Genomic DNA. Translation: AAD13687.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2125. XamI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019072. Restrct_endonuc_II_XamI.
[Graphical view]
PfamPF09572. RE_XamI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2X1_XANCR
AccessionPrimary (citable) accession number: P96189
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries