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P96188 (MTX1_XANCR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase XamI

Short name=M.XamI
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase XamI
Gene names
Name:xamIM
OrganismXanthomonas campestris pv. amaranthicola
Taxonomic identifier54735 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length527 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on a-5 on both strands, and protects the DNA from cleavage by the XamI endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 527527Modification methylase XamI
PRO_0000087983

Sequences

Sequence LengthMass (Da)Tools
P96188 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: B80C43CE684D8956

FASTA52757,535
        10         20         30         40         50         60 
MSMQTEQELV ALCLALIKDQ DSLSAAEKKL TKTTPVSALK PREIDTIRKR SRAGLTHSAN 

        70         80         90        100        110        120 
VLFDPLGHRA PRAGAVYTPA PIVRSMMTWL AAQGSPARIV DPGAGSGRFI LAAGEAFPDA 

       130        140        150        160        170        180 
QLVAVEMDPL AALMLRANLS ARGWTDRATV MVKDYREVKL PPCAGITAFI GNPPYVRHHD 

       190        200        210        220        230        240 
IGEDWKAWYA SNFAGYGIKA SALAGLHLHF FLQTRLLAKA GDVGAFITSA EWMDVNYGSA 

       250        260        270        280        290        300 
LRRLLLDELG GIALHVLEPT VEAFPGTATT AAIACFRVGE TARPVRVRFI DELTNLNGLT 

       310        320        330        340        350        360 
KGTDIPREQL QAASRWSIIV RPSAPAMAGD IELGELFRVH RGQVTGANGI WIAGEHAQGL 

       370        380        390        400        410        420 
PDRVKMPAVT KAKDLIQAGA HLNSAEVLRR VIDLPTDLDD FTKEERRRIS SFLSWAKLHG 

       430        440        450        460        470        480 
ADQSYIAQHR RAWWSVGLKA PAPILCTYMA RRPPQFTLNA CDARHINIAH GLYPREPLAA 

       490        500        510        520 
GIMASLVTWL NKNINTGSGR TYAGGLTKFE PKEIERLRIP SLENLHA 

« Hide

References

[1]"Isolation and nucleotide sequence of the gene encoding the XamI DNA methyltransferase of Xanthomonas campestris pv. amaranthicola."
Gomez P., Ribas-Aparicio R.M., Pelaez A.I., Gomez A., Rodicio M.R.
Biochim. Biophys. Acta 1351:261-266(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 11645 / LMG 498 / NCPPB 570.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U77781 Genomic DNA. Translation: AAD13686.1.

3D structure databases

ProteinModelPortalP96188.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3526. M.XamI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PRINTSPR00507. N12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMTX1_XANCR
AccessionPrimary (citable) accession number: P96188
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries