Reviewed, UniProtKB/Swiss-Prot P96188 (MTX1_XANCR)

Names and origin

Protein names

Recommended name:
    Modification methylase XamI
      Short name=M.XamI
    EC=2.1.1.72
Alternative name(s):
    Adenine-specific methyltransferase XamI

Gene names

Name:

xamIM

Organism

Xanthomonas campestris pv. amaranthicola

Taxonomic identifier

54735 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on a-5 on both strands, and protects the DNA from cleavage by the XamI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW methylation Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Modification methylase XamI		PRO_0000087983

Sequences

	Sequence		Length	Mass (Da)
	P96188-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: B80C43CE684D8956	FASTA	527	57,535
	10 20 30 40 50 60 MSMQTEQELV ALCLALIKDQ DSLSAAEKKL TKTTPVSALK PREIDTIRKR SRAGLTHSAN 70 80 90 100 110 120 VLFDPLGHRA PRAGAVYTPA PIVRSMMTWL AAQGSPARIV DPGAGSGRFI LAAGEAFPDA 130 140 150 160 170 180 QLVAVEMDPL AALMLRANLS ARGWTDRATV MVKDYREVKL PPCAGITAFI GNPPYVRHHD 190 200 210 220 230 240 IGEDWKAWYA SNFAGYGIKA SALAGLHLHF FLQTRLLAKA GDVGAFITSA EWMDVNYGSA 250 260 270 280 290 300 LRRLLLDELG GIALHVLEPT VEAFPGTATT AAIACFRVGE TARPVRVRFI DELTNLNGLT 310 320 330 340 350 360 KGTDIPREQL QAASRWSIIV RPSAPAMAGD IELGELFRVH RGQVTGANGI WIAGEHAQGL 370 380 390 400 410 420 PDRVKMPAVT KAKDLIQAGA HLNSAEVLRR VIDLPTDLDD FTKEERRRIS SFLSWAKLHG 430 440 450 460 470 480 ADQSYIAQHR RAWWSVGLKA PAPILCTYMA RRPPQFTLNA CDARHINIAH GLYPREPLAA 490 500 510 520 GIMASLVTWL NKNINTGSGR TYAGGLTKFE PKEIERLRIP SLENLHA

References

[1]

"Isolation and nucleotide sequence of the gene encoding the XamI DNA methyltransferase of Xanthomonas campestris pv. amaranthicola." Gomez P., Ribas-Aparicio R.M., Pelaez A.I., Gomez A., Rodicio M.R. Biochim. Biophys. Acta 1351:261-266(1997) [PubMed: 9130589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 11645 / LMG 498 / NCPPB 570.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U77781 Genomic DNA. Translation: AAD13686.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3526. M.XamI.
Enzyme and pathway databases
BRENDA	2.1.1.72. 295350.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTX1_XANCR

Accession

Primary (citable) accession number: P96188

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	May 1, 1997
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)