P96116 (TROA_TREPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Periplasmic zinc-binding protein TroA

Alternative name(s):
Tromp-1

Gene names

Name:	troA
Synonyms:	troMP1
Ordered Locus Names:	TP_0163

Organism

Treponema pallidum (strain Nichols) [Complete proteome] [HAMAP]

Taxonomic identifier

243276 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Spirochaetes › Spirochaetia › Spirochaetales › Spirochaetaceae › Treponema › Treponema pallidum › Treponema pallidum subsp. pallidum

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of an ATP-driven transport system TroABCD for zinc. Substrate-binding protein involved in the transport of zinc across the cytoplasmic membrane.
Subunit structure	Monomer.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the bacterial solute-binding protein 9 family.
Caution	Was originally (Ref.1) thought to be an outer membrane protein with porin-like properties.
Sequence caution	The sequence AAA92353.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ion transport Transport Zinc transport
Cellular component	Periplasm
Domain	Signal
Ligand	Metal-binding Zinc
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: InterPro zinc ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Chain

23 – 308

286

Periplasmic zinc-binding protein TroA

PRO_0000031872

Sites

Metal binding

68

1

Zinc

Metal binding

133

1

Zinc

Metal binding

199

1

Zinc

Metal binding

279

1

Zinc

Secondary structure

1

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308

Helix Strand Turn

Details...

Beta strand

35 – 40

6

Helix

41 – 51

11

Helix

52 – 54

3

Beta strand

55 – 60

6

Turn

67 – 69

3

Helix

74 – 82

9

Beta strand

84 – 88

5

Turn

91 – 94

4

Helix

95 – 97

3

Helix

98 – 104

7

Beta strand

107 – 112

6

Helix

113 – 116

4

Helix

119 – 121

3

Turn

126 – 128

3

Helix

134 – 136

3

Helix

138 – 155

18

Helix

157 – 159

3

Helix

160 – 186

27

Helix

190 – 192

3

Beta strand

194 – 200

7

Helix

203 – 209

7

Beta strand

212 – 217

6

Beta strand

221 – 223

3

Helix

227 – 239

13

Beta strand

243 – 248

6

Helix

254 – 264

11

Turn

265 – 267

3

Beta strand

271 – 276

6

Beta strand

278 – 280

3

Helix

287 – 289

3

Helix

291 – 306

16

Sequences

	Sequence		Length	Mass (Da)
	P96116 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 2FDD8FF20D012B08	FASTA	308	33,570
	10 20 30 40 50 60 MIRERICACV LALGMLTGFT HAFGSKDAAA DGKPLVVTTI GMIADAVKNI AQGDVHLKGL 70 80 90 100 110 120 MGPGVDPHLY TATAGDVEWL GNADLILYNG LHLETKMGEV FSKLRGSRLV VAVSETIPVS 130 140 150 160 170 180 QRLSLEEAEF DPHVWFDVKL WSYSVKAVYE SLCKLLPGKT REFTQRYQAY QQQLDKLDAY 190 200 210 220 230 240 VRRKAQSLPA ERRVLVTAHD AFGYFSRAYG FEVKGLQGVS TASEASAHDM QELAAFIAQR 250 260 270 280 290 300 KLPAIFIESS IPHKNVEALR DAVQARGHVV QIGGELFSDA MGDAGTSEGT YVGMVTHNID TIVAALAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Porin activity and sequence analysis of a 31-kilodalton Treponema pallidum subsp. pallidum rare outer membrane protein (Tromp1)." Blanco D.R., Champion C.I., Exner M.M., Erdjument-Bromage H., Hancock R.E., Tempst P., Miller J.N., Lovett M.A. J. Bacteriol. 177:3556-3562(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nichols.
[2]	"Identification and transcriptional analysis of a Treponema pallidum operon encoding a putative ABC transport system, an iron-activated repressor protein homolog, and a glycolytic pathway enzyme homolog." Hardham J.M., Stamm L.V., Porcella S.F., Frye J.G., Barnes N.Y., Howell J.K., Mueller S.L., Radolf J.D., Weinstock G.M., Norris S.J. Gene 197:47-64(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Nichols.
[3]	"Complete genome sequence of Treponema pallidum, the syphilis spirochete." Fraser C.M., Norris S.J., Weinstock G.M., White O., Sutton G.G., Dodson R.J., Gwinn M.L., Hickey E.K., Clayton R.A., Ketchum K.A., Sodergren E., Hardham J.M., McLeod M.P., Salzberg S.L., Peterson J.D., Khalak H.G., Richardson D.L., Howell J.K. , Chidambaram M., Utterback T.R., McDonald L.A., Artiach P., Bowman C., Cotton M.D., Fujii C., Garland S.A., Hatch B., Horst K., Roberts K.M., Sandusky M., Weidman J.F., Smith H.O., Venter J.C. Science 281:375-388(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Nichols.
[4]	"Physicochemical evidence that Treponema pallidum TroA is a zinc-containing metalloprotein that lacks porin-like structure." Deka R.K., Lee Y.-H., Hagman K.E., Shevchenko D., Lingwood C.A., Hasemann C.A., Norgard M.V., Radolf J.D. J. Bacteriol. 181:4420-4423(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone." Lee Y.-H., Deka R.K., Norgard M.V., Radolf J.D., Hasemann C.A. Nat. Struct. Biol. 6:628-633(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 23-313.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U16363 Genomic DNA. Translation: AAA92353.1. Different initiation.
U55214 Genomic DNA. Translation: AAC45725.1.
AE000520 Genomic DNA. Translation: AAC65151.1.

PIR

A71360.

RefSeq

NP_218602.1. NC_000919.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1K0F	X-ray	2.50	A	32-308	[»]
1TOA	X-ray	1.80	A/B	23-308	[»]

ProteinModelPortal

P96116.

SMR

P96116. Positions 32-308.

ModBase

Search...

Protein-protein interaction databases

IntAct

P96116. 8 interactions.

STRING

243276.TP0163.

Protein family/group databases

TCDB

3.A.1.15.8. ATP-binding cassette (ABC) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC65151; AAC65151; TP_0163.

GeneID

2611122.

KEGG

tpa:TP0163.

PATRIC

20530293. VBITrePal57110_0178.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG0803.

KO

K11707.

OMA

GTYVGMM.

ProtClustDB

CLSK375142.

Family and domain databases

InterPro

IPR006129. AdhesinB.
IPR006128. Lipoprotein_4.
IPR006127. Perip_solute-bd_prot_fam.
[Graphical view]

Pfam

PF01297. SBP_bac_9. 1 hit.
[Graphical view]

PRINTS

PR00691. ADHESINB.
PR00690. ADHESNFAMILY.

ProtoNet

Search...

Other

EvolutionaryTrace

P96116.

Entry information

Entry name

TROA_TREPA

Accession

Primary (citable) accession number: P96116
Secondary accession number(s): Q56329

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program