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Protein

Protein PyrBI

Gene

pyrBI

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain (carB), Carbamoyl-phosphate synthase small chain (carA), Carbamoyl-phosphate synthase (glutamine-hydrolyzing) (Tmari_0554)
  2. Protein PyrBI (pyrBI)
  3. Dihydroorotase (pyrC), Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi483 – 4831ZincBy similarity
Metal bindingi488 – 4881ZincBy similarity
Metal bindingi512 – 5121ZincBy similarity
Metal bindingi515 – 5151ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-503.
UniPathwayiUPA00070; UER00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein PyrBI
Including the following 2 domains:
Aspartate carbamoyltransferase (EC:2.1.3.2)
Alternative name(s):
Aspartate transcarbamylase
Short name:
ATCase
Aspartate carbamoyltransferase regulatory region
Gene namesi
Name:pyrBI
Ordered Locus Names:TM_1642
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 527527Protein PyrBIPRO_0000113240Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243274.TM1642.

Structurei

3D structure databases

ProteinModelPortaliP96111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342Aspartate carbamoyltransferaseAdd
BLAST
Regioni343 – 35715LinkerAdd
BLAST
Regioni368 – 527160Aspartate carbamoyltransferase regulatory regionAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the ATCase/OTCase family.Curated
In the C-terminal section; belongs to the PyrI family.Curated

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
COG1781. LUCA.
InParanoidiP96111.
KOiK00608.
OMAiDTFNMLT.
OrthoDBiEOG6D8B62.

Family and domain databases

Gene3Di2.30.30.20. 1 hit.
3.30.70.140. 1 hit.
3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR020545. Asp_carbamoyltransf_reg_N.
IPR020542. Asp_carbamoyltrfase_reg_C.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
PF01948. PyrI. 1 hit.
PF02748. PyrI_C. 1 hit.
[Graphical view]
PRINTSiPR00101. ATCASE.
ProDomiPD006194. Asp_carbamoylTrfase_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53671. SSF53671. 1 hit.
SSF54893. SSF54893. 1 hit.
SSF57825. SSF57825. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96111-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRDFLGRTL AVIEDLSVEE QMFLYEKTRE LKQRWYSGED VSDFRIKKRN
60 70 80 90 100
VGIYIVFVEP STRTKESFIN AAKFHSGPNV KVNVFDSEHS SFNKQESYTD
110 120 130 140 150
TFSMLTGYSD YSIFVVRTRL EGVCRLLERR ISEFASRNGI EVPSFINAGD
160 170 180 190 200
GKHEHPTQEL LDEYTFLEQN GFDNSFIHVA LVGDLLHGRT VHSKVNGLKI
210 220 230 240 250
FKNVKVDLVA PEELMMPEHY VEKMKKNGFE VRIFSSIREY LDQKDVAKIW
260 270 280 290 300
YFTRLQLERM GEDILEKVHV LREAVTFKKE YLDALPEGVK FYHPLPRHKV
310 320 330 340 350
YPTIPNFLDT LPLNGWETQA RNGYWVRIVL LSMFGGALEA PFDTSKKEEK
360 370 380 390 400
PEEDFIIPAP ITHGSKGVQK EGKRGIKPIE NGTVIDHIAK GKTPEEIYST
410 420 430 440 450
ILKIRKILRL YDVDSADGIF RSSDGSFKGY ISLPDRYLSK KEIKKLSAIS
460 470 480 490 500
PNTTVNIIKN STVVEKYRIK LPPTIYGFEE LRCKNENCIT NPAHGENASP
510 520
SFVRNEKGQF ICEYCETPHS FEEIWSI
Length:527
Mass (Da):60,772
Last modified:May 30, 2000 - v2
Checksum:i24BBF883B3BFC03D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti525 – 5273WSI → T in CAA71345 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10300 Genomic DNA. Translation: CAA71345.1.
AE000512 Genomic DNA. Translation: AAD36709.1.
PIRiB72231.
RefSeqiNP_229442.1. NC_000853.1.
WP_004082131.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD36709; AAD36709; TM_1642.
GeneIDi897920.
KEGGitma:TM1642.
PATRICi23938258. VBITheMar51294_1661.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10300 Genomic DNA. Translation: CAA71345.1.
AE000512 Genomic DNA. Translation: AAD36709.1.
PIRiB72231.
RefSeqiNP_229442.1. NC_000853.1.
WP_004082131.1. NZ_CP011107.1.

3D structure databases

ProteinModelPortaliP96111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM1642.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD36709; AAD36709; TM_1642.
GeneIDi897920.
KEGGitma:TM1642.
PATRICi23938258. VBITheMar51294_1661.

Phylogenomic databases

eggNOGiENOG4105CXT. Bacteria.
COG0540. LUCA.
COG1781. LUCA.
InParanoidiP96111.
KOiK00608.
OMAiDTFNMLT.
OrthoDBiEOG6D8B62.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00116.
BioCyciMetaCyc:MONOMER-503.

Family and domain databases

Gene3Di2.30.30.20. 1 hit.
3.30.70.140. 1 hit.
3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR002082. Asp_carbamoyltransf.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR020545. Asp_carbamoyltransf_reg_N.
IPR020542. Asp_carbamoyltrfase_reg_C.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
PF01948. PyrI. 1 hit.
PF02748. PyrI_C. 1 hit.
[Graphical view]
PRINTSiPR00101. ATCASE.
ProDomiPD006194. Asp_carbamoylTrfase_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53671. SSF53671. 1 hit.
SSF54893. SSF54893. 1 hit.
SSF57825. SSF57825. 1 hit.
TIGRFAMsiTIGR00670. asp_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. van de Casteele M.F.J., Chen P., van Vliet F., Legrain C., Cunin R., Glansdorff N.
    Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiPYRB_THEMA
AccessioniPrimary (citable) accession number: P96111
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: April 13, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.