Reviewed, UniProtKB/Swiss-Prot P96084 (PIP_THEAC)

Names and origin

Protein names

Recommended name:
    Proline iminopeptidase
      Short name=PIP
    EC=3.4.11.5
Alternative name(s):
    Prolyl aminopeptidase
      Short name=PAP
    Tricorn protease-interacting factor F1

Gene names

Name:	pip
Ordered Locus Names:	Ta0830

Organism

Thermoplasma acidophilum [Complete proteome] [HAMAP]

Taxonomic identifier

2303 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Thermoplasmata › Thermoplasmatales › Thermoplasmataceae › Thermoplasma

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. Proteases F1, F2 and F3 degrade oligopeptides produced by Tricorn (themselves probably produced by the proteasome) yielding free amino acids.
Catalytic activity	Release of N-terminal proline from a peptide.
Subunit structure	Part of the tricorn proteolytic complex.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 293

293

Proline iminopeptidase

PRO_0000080851

Sites

Active site

105

1

Nucleophile

Active site

244

1

By similarity

Active site

271

1

Proton donor

Secondary structure

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293

Helix Strand Turn

Details...

Beta strand

6 – 9

4

Beta strand

13 – 15

3

Beta strand

18 – 22

5

Beta strand

29 – 34

6

Turn

37 – 39

3

Helix

42 – 52

11

Turn

53 – 55

3

Beta strand

56 – 61

6

Helix

74 – 76

3

Helix

79 – 94

16

Beta strand

99 – 104

6

Helix

106 – 118

13

Helix

119 – 121

3

Beta strand

122 – 129

8

Helix

134 – 146

13

Helix

150 – 159

10

Turn

160 – 163

4

Helix

168 – 181

14

Helix

190 – 201

12

Helix

204 – 208

5

Turn

219 – 222

4

Turn

226 – 228

3

Helix

229 – 231

3

Beta strand

236 – 241

6

Helix

248 – 257

10

Beta strand

262 – 266

5

Helix

273 – 276

4

Helix

278 – 290

13

Sequences

	Sequence		Length	Mass (Da)
	P96084-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 4FD91EE29668FB65	FASTA	293	33,487
	10 20 30 40 50 60 MDQECIENYA KVNGIYIYYK LCKAPEEKAK LMTMHGGPGM SHDYLLSLRD MTKEGITVLF 70 80 90 100 110 120 YDQFGCGRSE EPDQSKFTID YGVEEAEALR SKLFGNEKVF LMGSSYGGAL ALAYAVKYQD 130 140 150 160 170 180 HLKGLIVSGG LSSVPLTVKE MNRLIDELPA KYRDAIKKYG SSGSYENPEY QEAVNYFYHQ 190 200 210 220 230 240 HLLRSEDWPP EVLKSLEYAE RRNVYRIMNG PNEFTITGTI KDWDITDKIS AIKIPTLITV 250 260 270 280 290 GEYDEVTPNV ARVIHEKIAG SELHVFRDCS HLTMWEDREG YNKLLSDFIL KHL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Tricorn protease (TRI) interacting factor 1 from Thermoplasma acidophilum is a proline iminopeptidase." Tamura T., Tamura N., Lottspeich F., Baumeister W. FEBS Lett. 398:101-105(1996) [PubMed: 8946961] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.
[2]	"The genome sequence of the thermoacidophilic scavenger Thermoplasma acidophilum." Ruepp A., Graml W., Santos-Martinez M.-L., Koretke K.K., Volker C., Mewes H.-W., Frishman D., Stocker S., Lupas A.N., Baumeister W. Nature 407:508-513(2000) [PubMed: 11029001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25905 / AMRC-C165 / DSM 1728 / IFO 15155 / JCM 9062.
[3]	"Structures of the tricorn-interacting aminopeptidase F1 with different ligands explain its catalytic mechanism." Goettig P., Groll M., Kim J.S., Huber R., Brandstetter H. EMBO J. 21:5343-5352(2002) [PubMed: 12374735] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U72710 Genomic DNA. Translation: AAC44636.1.
AL445065 Genomic DNA. Translation: CAC11959.1.

PIR

T37465.

RefSeq

NP_394291.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MT3	X-ray	2.00	A	1-293	[»]
1MTZ	X-ray	1.80	A	1-293	[»]
1MU0	X-ray	2.40	A	1-293	[»]
1XQV	X-ray	2.30	A	1-293	[»]
1XQW	X-ray	2.00	A	1-293	[»]
1XQX	X-ray	2.10	A	1-293	[»]
1XQY	X-ray	3.20	A	1-293	[»]
1XRL	X-ray	1.82	A	1-293	[»]
1XRM	X-ray	2.70	A	1-293	[»]
1XRN	X-ray	2.80	A	1-293	[»]
1XRO	X-ray	1.80	A	1-293	[»]
1XRP	X-ray	2.30	A	1-293	[»]
1XRQ	X-ray	2.80	A	1-293	[»]
1XRR	X-ray	2.40	A	1-293	[»]

ModBase

Search...

Protein family/group databases

MEROPS

S33.005.

Genome annotation databases

GeneID

1456375.

KEGG

tac:Ta0830.

NMPDR

fig|273075.1.peg.813.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HBG515521.

OMA

PTEFHVI.

Enzyme and pathway databases

BioCyc

TACI273075:TA0830-MONOMER.

BRENDA

3.4.11.5. 435.

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR005945. Pept_S33_TRI_F1.
IPR002410. Peptidase_S33.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PIRSF

PIRSF005539. Pept_S33_TRI_F1. 1 hit.

PRINTS

PR00793. PROAMNOPTASE.

TIGRFAMs

TIGR01250. pro_imino_pep_2. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PIP_THEAC

Accession

Primary (citable) accession number: P96084

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1997
Last modified:	February 9, 2010
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)