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Protein

Proline iminopeptidase

Gene

pip

Organism
Thermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves H-Pro-AMC as well as a wide spectrum of amino acid substrates and several peptide substrates without a proline at the N-terminus. Proteases F1, F2 and F3 degrade oligopeptides produced by Tricorn (themselves probably produced by the proteasome) yielding free amino acids.

Catalytic activityi

Release of N-terminal proline from a peptide.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105Nucleophile1
Active sitei244By similarity1
Active sitei271Proton donor1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Protein family/group databases

ESTHERitheac-pip. Proline_iminopeptidase.
MEROPSiS33.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline iminopeptidase (EC:3.4.11.5)
Short name:
PIP
Alternative name(s):
Prolyl aminopeptidase
Short name:
PAP
Tricorn protease-interacting factor F1
Gene namesi
Name:pip
Ordered Locus Names:Ta0830
OrganismiThermoplasma acidophilum (strain ATCC 25905 / DSM 1728 / JCM 9062 / NBRC 15155 / AMRC-C165)
Taxonomic identifieri273075 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermoplasmataThermoplasmatalesThermoplasmataceaeThermoplasma
Proteomesi
  • UP000001024 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000808511 – 293Proline iminopeptidaseAdd BLAST293

Proteomic databases

PRIDEiP96084.

Interactioni

Subunit structurei

Part of the tricorn proteolytic complex.

Protein-protein interaction databases

STRINGi273075.Ta0830.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 12Combined sources7
Beta strandi15 – 22Combined sources8
Beta strandi28 – 34Combined sources7
Turni37 – 39Combined sources3
Helixi43 – 54Combined sources12
Beta strandi56 – 61Combined sources6
Helixi74 – 76Combined sources3
Helixi79 – 94Combined sources16
Beta strandi99 – 104Combined sources6
Helixi106 – 118Combined sources13
Helixi119 – 121Combined sources3
Beta strandi122 – 129Combined sources8
Helixi134 – 146Combined sources13
Helixi150 – 162Combined sources13
Helixi168 – 181Combined sources14
Beta strandi185 – 187Combined sources3
Helixi190 – 201Combined sources12
Helixi204 – 208Combined sources5
Beta strandi211 – 214Combined sources4
Turni219 – 222Combined sources4
Turni226 – 228Combined sources3
Helixi229 – 231Combined sources3
Beta strandi236 – 241Combined sources6
Helixi248 – 257Combined sources10
Beta strandi262 – 266Combined sources5
Helixi273 – 276Combined sources4
Helixi278 – 290Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MT3X-ray2.00A1-293[»]
1MTZX-ray1.80A1-293[»]
1MU0X-ray2.40A1-293[»]
1XQVX-ray2.30A1-293[»]
1XQWX-ray2.00A1-293[»]
1XQXX-ray2.10A1-293[»]
1XQYX-ray3.20A1-293[»]
1XRLX-ray1.82A1-293[»]
1XRMX-ray2.70A1-293[»]
1XRNX-ray2.80A1-293[»]
1XROX-ray1.80A1-293[»]
1XRPX-ray2.30A1-293[»]
1XRQX-ray2.80A1-293[»]
1XRRX-ray2.40A1-293[»]
ProteinModelPortaliP96084.
SMRiP96084.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96084.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S33 family.Curated

Phylogenomic databases

eggNOGiarCOG01648. Archaea.
COG0596. LUCA.
HOGENOMiHOG000026004.
KOiK01259.
OMAiWGPNEYT.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022742. Hydrolase_4.
IPR002410. Peptidase_S33.
IPR005945. Pro_imino_pep.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
PIRSFiPIRSF005539. Pept_S33_TRI_F1. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00793. PROAMNOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01250. pro_imino_pep_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P96084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQECIENYA KVNGIYIYYK LCKAPEEKAK LMTMHGGPGM SHDYLLSLRD
60 70 80 90 100
MTKEGITVLF YDQFGCGRSE EPDQSKFTID YGVEEAEALR SKLFGNEKVF
110 120 130 140 150
LMGSSYGGAL ALAYAVKYQD HLKGLIVSGG LSSVPLTVKE MNRLIDELPA
160 170 180 190 200
KYRDAIKKYG SSGSYENPEY QEAVNYFYHQ HLLRSEDWPP EVLKSLEYAE
210 220 230 240 250
RRNVYRIMNG PNEFTITGTI KDWDITDKIS AIKIPTLITV GEYDEVTPNV
260 270 280 290
ARVIHEKIAG SELHVFRDCS HLTMWEDREG YNKLLSDFIL KHL
Length:293
Mass (Da):33,487
Last modified:May 1, 1997 - v1
Checksum:i4FD91EE29668FB65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72710 Genomic DNA. Translation: AAC44636.1.
AL445065 Genomic DNA. Translation: CAC11959.1.
PIRiT37465.

Genome annotation databases

EnsemblBacteriaiCAC11959; CAC11959; CAC11959.
KEGGitac:Ta0830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72710 Genomic DNA. Translation: AAC44636.1.
AL445065 Genomic DNA. Translation: CAC11959.1.
PIRiT37465.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MT3X-ray2.00A1-293[»]
1MTZX-ray1.80A1-293[»]
1MU0X-ray2.40A1-293[»]
1XQVX-ray2.30A1-293[»]
1XQWX-ray2.00A1-293[»]
1XQXX-ray2.10A1-293[»]
1XQYX-ray3.20A1-293[»]
1XRLX-ray1.82A1-293[»]
1XRMX-ray2.70A1-293[»]
1XRNX-ray2.80A1-293[»]
1XROX-ray1.80A1-293[»]
1XRPX-ray2.30A1-293[»]
1XRQX-ray2.80A1-293[»]
1XRRX-ray2.40A1-293[»]
ProteinModelPortaliP96084.
SMRiP96084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273075.Ta0830.

Protein family/group databases

ESTHERitheac-pip. Proline_iminopeptidase.
MEROPSiS33.005.

Proteomic databases

PRIDEiP96084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC11959; CAC11959; CAC11959.
KEGGitac:Ta0830.

Phylogenomic databases

eggNOGiarCOG01648. Archaea.
COG0596. LUCA.
HOGENOMiHOG000026004.
KOiK01259.
OMAiWGPNEYT.

Miscellaneous databases

EvolutionaryTraceiP96084.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
IPR022742. Hydrolase_4.
IPR002410. Peptidase_S33.
IPR005945. Pro_imino_pep.
[Graphical view]
PfamiPF12146. Hydrolase_4. 1 hit.
[Graphical view]
PIRSFiPIRSF005539. Pept_S33_TRI_F1. 1 hit.
PRINTSiPR00111. ABHYDROLASE.
PR00793. PROAMNOPTASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR01250. pro_imino_pep_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPIP_THEAC
AccessioniPrimary (citable) accession number: P96084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.