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P96077 (RF1_THET8) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peptide chain release factor 1

Short name=RF-1
Gene names
Name:prfA
Ordered Locus Names:TTHA1940
OrganismThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579) [Reference proteome] [HAMAP]
Taxonomic identifier300852 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA By similarity. HAMAP-Rule MF_00093

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00093.

Post-translational modification

Methylated by PrmC. Methylation increases the termination efficiency of RF1 By similarity. HAMAP-Rule MF_00093

Sequence similarities

Belongs to the prokaryotic/mitochondrial release factor family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   PTMMethylation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontranslation release factor activity, codon specific

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Peptide chain release factor 1 HAMAP-Rule MF_00093
PRO_0000177762

Amino acid modifications

Modified residue2301N5-methylglutamine By similarity

Sequences

Sequence LengthMass (Da)Tools
P96077 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 49868D246BB3339E

FASTA35440,093
        10         20         30         40         50         60 
MLDKLDRLEE EYRELEALLS DPEVLKDKGR YQSLSRRYAE MGEVIGLIRE YRKVLEDLEQ 

        70         80         90        100        110        120 
AESLLDDPEL KEMAKAEREA LLARKEALEK ELERHLLPKD PMDERDAIVE IRAGTGGEEA 

       130        140        150        160        170        180 
ALFARDLFNM YLRFAEEMGF ETEVLDSHPT DLGGFSKVVF EVRGPGAYGT FKYESGVHRV 

       190        200        210        220        230        240 
QRVPVTETQG RIHTSTATVA VLPKAEEEDF ALNMDEIRID VMRASGPGGQ GVNTTDSAVR 

       250        260        270        280        290        300 
VVHLPTGIMV TCQDSRSQIK NREKALMILR SRLLEMKRAE EAERLRKTRL AQIGTGERSE 

       310        320        330        340        350 
KIRTYNFPQS RVTDHRIGFT THDLEGVLSG HLTPILEALK RADQERQLAA LAEG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning of RF-1 gene from Thermus thermophilus HB8."
Ito K., Ebihara K., Nakamura Y.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Thermus thermophilus HB8."
Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HB8 / ATCC 27634 / DSM 579.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87366 Genomic DNA. Translation: BAA13349.1.
AP008226 Genomic DNA. Translation: BAD71763.1.
RefSeqYP_145206.1. NC_006461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2B64X-ray5.90Y1-354[»]
ProteinModelPortalP96077.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING300852.TTHA1940.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD71763; BAD71763; BAD71763.
GeneID3169506.
KEGGttj:TTHA1940.
PATRIC23958859. VBITheThe93045_1911.

Phylogenomic databases

eggNOGCOG0216.
HOGENOMHOG000074815.
KOK02835.
OMAYGQLKFE.
OrthoDBEOG6TN48J.
PhylomeDBP96077.

Enzyme and pathway databases

BioCycTTHE300852:GH8R-1990-MONOMER.

Family and domain databases

Gene3D3.30.160.20. 1 hit.
HAMAPMF_00093. Rel_fac_1.
InterProIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsTIGR00019. prfA. 1 hit.
PROSITEPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP96077.

Entry information

Entry nameRF1_THET8
AccessionPrimary (citable) accession number: P96077
Secondary accession number(s): Q5SGZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references