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Protein

Peptide chain release factor 1

Gene

prfA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA.By similarity

GO - Molecular functioni

  1. translation release factor activity, codon specific Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1990-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide chain release factor 1
Short name:
RF-1
Gene namesi
Name:prfA
Ordered Locus Names:TTHA1940
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
ProteomesiUP000000532: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Peptide chain release factor 1PRO_0000177762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei230 – 2301N5-methylglutamineBy similarity

Post-translational modificationi

Methylated by PrmC. Methylation increases the termination efficiency of RF1 (By similarity).By similarity

Keywords - PTMi

Methylation

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1940.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V4RX-ray5.90AY1-354[»]
ProteinModelPortaliP96077.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP96077.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0216.
HOGENOMiHOG000074815.
KOiK02835.
OMAiWKVEIVS.
OrthoDBiEOG6TN48J.
PhylomeDBiP96077.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P96077-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDKLDRLEE EYRELEALLS DPEVLKDKGR YQSLSRRYAE MGEVIGLIRE
60 70 80 90 100
YRKVLEDLEQ AESLLDDPEL KEMAKAEREA LLARKEALEK ELERHLLPKD
110 120 130 140 150
PMDERDAIVE IRAGTGGEEA ALFARDLFNM YLRFAEEMGF ETEVLDSHPT
160 170 180 190 200
DLGGFSKVVF EVRGPGAYGT FKYESGVHRV QRVPVTETQG RIHTSTATVA
210 220 230 240 250
VLPKAEEEDF ALNMDEIRID VMRASGPGGQ GVNTTDSAVR VVHLPTGIMV
260 270 280 290 300
TCQDSRSQIK NREKALMILR SRLLEMKRAE EAERLRKTRL AQIGTGERSE
310 320 330 340 350
KIRTYNFPQS RVTDHRIGFT THDLEGVLSG HLTPILEALK RADQERQLAA

LAEG
Length:354
Mass (Da):40,093
Last modified:May 1, 1997 - v1
Checksum:i49868D246BB3339E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87366 Genomic DNA. Translation: BAA13349.1.
AP008226 Genomic DNA. Translation: BAD71763.1.
RefSeqiWP_011173949.1. NC_006461.1.
YP_145206.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71763; BAD71763; BAD71763.
GeneIDi3169506.
KEGGittj:TTHA1940.
PATRICi23958859. VBITheThe93045_1911.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87366 Genomic DNA. Translation: BAA13349.1.
AP008226 Genomic DNA. Translation: BAD71763.1.
RefSeqiWP_011173949.1. NC_006461.1.
YP_145206.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4V4RX-ray5.90AY1-354[»]
ProteinModelPortaliP96077.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1940.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71763; BAD71763; BAD71763.
GeneIDi3169506.
KEGGittj:TTHA1940.
PATRICi23958859. VBITheThe93045_1911.

Phylogenomic databases

eggNOGiCOG0216.
HOGENOMiHOG000074815.
KOiK02835.
OMAiWKVEIVS.
OrthoDBiEOG6TN48J.
PhylomeDBiP96077.

Enzyme and pathway databases

BioCyciTTHE300852:GH8R-1990-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP96077.

Family and domain databases

Gene3Di3.30.160.20. 1 hit.
HAMAPiMF_00093. Rel_fac_1.
InterProiIPR014720. dsRNA-bd_dom.
IPR005139. PCRF.
IPR000352. Pep_chain_release_fac_I_II.
IPR004373. PrfA.
[Graphical view]
PfamiPF03462. PCRF. 1 hit.
PF00472. RF-1. 1 hit.
[Graphical view]
SMARTiSM00937. PCRF. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00019. prfA. 1 hit.
PROSITEiPS00745. RF_PROK_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of RF-1 gene from Thermus thermophilus HB8."
    Ito K., Ebihara K., Nakamura Y.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete genome sequence of Thermus thermophilus HB8."
    Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T., Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HB8 / ATCC 27634 / DSM 579.

Entry informationi

Entry nameiRF1_THET8
AccessioniPrimary (citable) accession number: P96077
Secondary accession number(s): Q5SGZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: March 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.