P96041 (SYA_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	SSO0341

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000075277
Sites
	Metal binding	604	1	Zinc By similarity
	Metal binding	608	1	Zinc By similarity
	Metal binding	708	1	Zinc By similarity
	Metal binding	712	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P96041 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 6CB15A31210E153F	FASTA	900	102,980
	10 20 30 40 50 60 MKASEEEYKL NFFIKNDFKR KICKSCQTPF WTKDDKKEYC SDIPCTDYYF FDINIKSQPL 70 80 90 100 110 120 TVKEAREKFL SFFEKRGHTR IPPKPVLARW REDLYLTIAS IVDFQPHVTS GLVPPPANPL 130 140 150 160 170 180 VVSQPSIRLE DIDNVGVTFG RHLTTFEMAA HHAFNYPDRY VYWKDETTAY ATEFFTKELG 190 200 210 220 230 240 IPEEELNFKE SWWEGGGNAG PCLEVTVGGL ELATLVFMQY KITDNGDYIP LKLKIVDTGY 250 260 270 280 290 300 GVERIAWVTQ KTPSAFHAIY GNLVYKFFDK IGVAYIDETL LKVASRFAGK IDPDNPDTIK 310 320 330 340 350 360 IHRQMVSKEL GIDIKNVEEE LDRAAKVFQI LDHTKTIMLM LADGLVPSNS GEGYLGRLVI 370 380 390 400 410 420 RRALKVLRLL KSDVRLYELV KEQIGFWKED FPQVLKNKDY ILDAVELEQQ RFEKILEKVP 430 440 450 460 470 480 SIASTLARKS EITTEDLIQV YDSNGVPPDL LEEELKKKRV KFELPRNFYA LVAKRHQTST 490 500 510 520 530 540 IKNVYDKVKL PKDLLEYITT LQPTEKLYYK DQYMRSFEGK VLGIYKNYLI LDKTTFYPEG 550 560 570 580 590 600 GGQLGDTGLI IDEKSSKRYE VVDTQKVNDV IIHVLKEEPS TIKVGDNVRG EINWERRYRL 610 620 630 640 650 660 MRHHTVTHVI LAAAKKVLGD HIWQAGAEKT PEKGRLDITH HKALTEEEVK LIENYANSVI 670 680 690 700 710 720 SDRRPVKPLE MNRMEAEMKY GVSIYEGGVP NSATIRLLEI KDWDIESCGG THVSNTSEIG 730 740 750 760 770 780 AVKIINVERI QDGIIRLEYV AGPALVDYIR ETEAKIAEAS KIIGTSPDQL PSRLRRILNE 790 800 810 820 830 840 VEKKNNLIIQ YRRIVETELL NSLKPYEING NKIYIIEGLN DEEENKEILR KLTSTDNTIA 850 860 870 880 890 900 ISISDNRLQI ATSKNMRVDK IVEELLKGGG KGGGKGTFAN VILSSKKSKE EIIDIVRKSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"Nucleotide sequence of a gene cluster encoding NusG and the L11-L1-L10-L12 ribosomal proteins from the thermophilic archaeon Sulfolobus solfataricus." Geiger M., Groebner P., Piendl W. Biochim. Biophys. Acta 1340:170-177(1997) [PubMed: 9252104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-174.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE006641 Genomic DNA. Translation: AAK40671.1. U85262 Genomic DNA. Translation: AAB99528.1.
PIR	H90176.
RefSeq	NP_341881.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P96041.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1455481.
GenomeReviews	Gene locus SSO0341 in contig AE006641_GR.
KEGG	sso:SSO0341.
NMPDR	fig|273057.1.peg.304.
Phylogenomic databases
HOGENOM	HBG392147.
OMA	GESKTDQ.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0341-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_SULSO

Accession

Primary (citable) accession number: P96041

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program