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Protein

L-proline cis-3-hydroxylase 1

Gene
N/A
Organism
Streptomyces sp.
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-3-hydroxy-L-proline (cis-3-Hyp). D-proline, trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, cis-4-hydroxy-D-proline, and 3,4-dehydro-DL-proline are not substrates.2 Publications

Catalytic activityi

L-proline + 2-oxoglutarate + O2 = cis-3-hydroxy-L-proline + succinate + CO2.2 Publications

Cofactori

Fe2+2 PublicationsNote: Binds 1 Fe2+ ion.2 Publications

Enzyme regulationi

Inhibited by metal ions such as Co2+, Zn2+, Ni2+ or Cu2+. Is also inhibited by EDTA in vitro. Unlike the procollagen-proline cis-3- and trans-4-hydroxylases from mammals, does not necessarily require L-ascorbate for activity although it does increase the activity of the enzyme.2 Publications

Kineticsi

Comparison of both proline 3-hydroxylase isozymes shows that type II enzyme has 1.8-fold higher activity than type I upon L-proline, and 24-fold higher activity upon L-2-azetidinecarboxylic acid.

  1. KM=0.20 mM for L-proline1 Publication
  2. KM=0.083 mM for 2-oxoglutarate1 Publication

    pH dependencei

    Optimum pH is 6.0. Is stable from pH 5.5 to 8.5.1 Publication

    Temperature dependencei

    Optimum temperature is 35 degrees Celsius. Is stable below 30 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi107IronBy similarity1
    Metal bindingi109IronBy similarity1
    Metal bindingi158IronBy similarity1
    Binding sitei1682-oxoglutarateSequence analysis1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-proline cis-3-hydroxylase 1 (EC:1.14.11.28)
    Short name:
    P3H 1
    Alternative name(s):
    Proline 3-hydroxylase 1
    Proline 3-hydroxylase type I
    OrganismiStreptomyces sp.
    Taxonomic identifieri1931 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003934261 – 290L-proline cis-3-hydroxylase 1Add BLAST290

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP96010.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    KOiK20149.

    Family and domain databases

    Gene3Di1.10.1720.10. 1 hit.
    2.60.120.330. 1 hit.
    InterProiIPR007803. Asp/Arg/Pro-Hydrxlase.
    IPR027443. IPNS-like.
    IPR008035. Pro_3_hydrox_C.
    [Graphical view]
    PfamiPF05118. Asp_Arg_Hydrox. 1 hit.
    PF05373. Pro_3_hydrox_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P96010-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRSHILGRIE LDQERLGRDL EYLATVPTVE EEYDEFSNGF WKNIPLYNAS
    60 70 80 90 100
    GGSEDRLYRD LEGSPAQPTK HAEQVPYLNE IITTVYNGER LQMARTRNLK
    110 120 130 140 150
    NAVVIPHRDF VELDRELDQY FRTHLMLEDS PLAFHSDDDT VIHMRAGEIW
    160 170 180 190 200
    FLDAAAVHSA VNFAEFSRQS LCVDLAFDGA FDEKEAFADA TVYAPNLSPD
    210 220 230 240 250
    VRERKPFTKE REAGILALSG VIGRENFRDI LFLLSKVHYT YDVHPGETFE
    260 270 280 290
    WLVSVSKGAG DDKMVEKAER IRDFAIGARA LGERFSLTTW
    Length:290
    Mass (Da):33,154
    Last modified:May 1, 1997 - v1
    Checksum:i7AC1A538D0FB294C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB007189 Genomic DNA. Translation: BAA22406.1.

    Genome annotation databases

    KEGGiag:BAA22406.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB007189 Genomic DNA. Translation: BAA22406.1.

    3D structure databases

    ProteinModelPortaliP96010.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:BAA22406.

    Phylogenomic databases

    KOiK20149.

    Family and domain databases

    Gene3Di1.10.1720.10. 1 hit.
    2.60.120.330. 1 hit.
    InterProiIPR007803. Asp/Arg/Pro-Hydrxlase.
    IPR027443. IPNS-like.
    IPR008035. Pro_3_hydrox_C.
    [Graphical view]
    PfamiPF05118. Asp_Arg_Hydrox. 1 hit.
    PF05373. Pro_3_hydrox_C. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiP3H1_STRSQ
    AccessioniPrimary (citable) accession number: P96010
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: May 1, 1997
    Last modified: October 5, 2016
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.