P96000 (GPT_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
EC=2.7.8.15

Alternative name(s):
GlcNAc-1-P transferase
Short name=G1PT
Short name=GPT
N-acetylglucosamine-1-phosphate transferase

Gene names

Name:	gnpTA
Ordered Locus Names:	SSO0060
ORF Names:	C05011

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.
Enzyme regulation	Inhibited by tunicamycin By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the glycosyltransferase 4 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Inferred from electronic annotation. Source: EC phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: InterPro transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Putative UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase		PRO_0000108767
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Transmembrane	46 – 66	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	102 – 122	21	Helical; Potential
	Transmembrane	123 – 143	21	Helical; Potential
	Transmembrane	160 – 180	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	222 – 242	21	Helical; Potential
	Transmembrane	295 – 315	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P96000 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 41F58B03A3269F7D	FASTA	322	35,252
	10 20 30 40 50 60 MRILAILLPI LISFFISYIT TVWVIRQAKK SRFVGKDINK PDKPEIPLLG GIGIIAGFIA 70 80 90 100 110 120 GSFSLLLTDV RSERVIPAVI LSSLLIAFLG LLDDIFNVRQ SVRAFLPIFA SVPLIVYSVG 130 140 150 160 170 180 HSIISIPFLG PINFGIFYYI IIIPFALTIT SNAFNMLEGL NGLGVGMGII MLSALAYIGL 190 200 210 220 230 240 THTGPTYQAG LIALSAIFSL SAFLIFNKYP AKIFPGNVGT YFIGALIGAI GIAGFMYTAL 250 260 270 280 290 300 AILYIPYVVE FILKLRTNFK GVSFGKVDSS GRLYWDEKPH SLTHIVMKMG RFKEYQVVII 310 320 LWGMEAIFAV IAVILQTTTI VI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2." Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y., Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F., Ragan M.A., Charlebois R.L. Mol. Microbiol. 22:175-191(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08257 Genomic DNA. Translation: CAA69542.1. AE006641 Genomic DNA. Translation: AAK40422.1.
PIR	S75428.
RefSeq	NP_341632.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P96000.
ModBase	Search...
Protein-protein interaction databases
STRING	273057.SSO0060.
Protocols and materials databases
DNASU	1455313.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK40422; AAK40422; SSO0060.
GeneID	1455313.
KEGG	sso:SSO0060.
Phylogenomic databases
eggNOG	COG0472.
HOGENOM	HOG000227679.
KO	K01001.
OMA	IPFGGPM.
ProtClustDB	CLSK802540.
Family and domain databases
InterPro	IPR000715. Glycosyl_transferase_4. [Graphical view]
PANTHER	PTHR22926. PTHR22926. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPT_SULSO

Accession

Primary (citable) accession number: P96000

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program