P95963 (AMPM_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	SSO0098
ORF Names:	C04024, C05_012

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Methionine aminopeptidase		PRO_0000148981
Sites
	Metal binding	85	1	Cobalt 1 By similarity
	Metal binding	96	1	Cobalt 1 By similarity
	Metal binding	96	1	Cobalt 2 By similarity
	Metal binding	156	1	Cobalt 2 By similarity
	Metal binding	189	1	Cobalt 2 By similarity
	Metal binding	284	1	Cobalt 1 By similarity
	Metal binding	284	1	Cobalt 2 By similarity
	Binding site	65	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P95963 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 3E156B383EF516F0	FASTA	301	33,252
	10 20 30 40 50 60 MTEDELNKLL LAGKIAAKAR DEVSLDVKAS AKVLDICEEV ESIIIENKAF PSFPCNISIN 70 80 90 100 110 120 SEAAHYSPTI NDEKRIPEGA VVKLDLGAHI DGFISDTAIT ISLDSRYQRL LDASKTALEA 130 140 150 160 170 180 AITNFKAGLS IGEIGRVIEK VIRAQGYKPI RNLGGHLIRR YELHAGVFIP NVYERGLGVI 190 200 210 220 230 240 QSDSVYAIEP FATDGGGEVV EGKSITIYSL KNPNIKGLSS RENELIDFIY TRFNYLPFSE 250 260 270 280 290 300 RWLKEFSTNV DELRNNIKNL VKKGALRGYP ILIEIKKGVV SQFEHTVIVK GDSIIVSTKS L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2." Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y., Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F., Ragan M.A., Charlebois R.L. Mol. Microbiol. 22:175-191(1996) [PubMed: 8899719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08257 Genomic DNA. Translation: CAA69553.1. AE006641 Genomic DNA. Translation: AAK40454.1.
PIR	S75391.
RefSeq	NP_341664.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P95963.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1453282.
GenomeReviews	Gene locus SSO0098 in contig AE006641_GR.
KEGG	sso:SSO0098.
NMPDR	fig|273057.1.peg.87.
Phylogenomic databases
HOGENOM	HBG318153.
OMA	IEPFATN.
ProtClustDB	PRK08671.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0098-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002468. Pept_M24A_MAP2. IPR018349. Pept_M24A_MAP2_BS. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 2 hits. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01265.
PANTHER	PTHR10804:SF9. Pept_M24A_MAP2. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00501. Met_pdase_II. 1 hit.
PROSITE	PS01202. MAP_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_SULSO

Accession

Primary (citable) accession number: P95963

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	December 14, 2011
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program