P95961 (SYFA_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	SSO0100
ORF Names:	C04_022

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00282
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00282
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00282.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00282		PRO_0000126820

Sequences

	Sequence		Length	Mass (Da)
	P95961 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 527E274CC79944E2	FASTA	468	54,102
	10 20 30 40 50 60 MISMLSENEA KILFFLKDLK KTNSVELAIK MGIPESSVLS LIELLREKGY VKTEVKSEKH 70 80 90 100 110 120 YVLTEEGRKR KESGLPEDIL INTLNGQEKD LNEIKNILNK DFNIAISWAK RKGLIDIKEG 130 140 150 160 170 180 KVIPKVKTYM SSEYLALSNL ERADSNTINL LKKRGLIEEK ERKIVSVELI KEPKESEIGI 190 200 210 220 230 240 SNLNRELIIS GEWKKYKLKK YNVEAFPPYY TISKKHYFRE FLEKVKDIMI SLGFKEINTG 250 260 270 280 290 300 YIEMEFYNFD LLFQPQDHPA REIHDSFSVE GSGKIEDKDL LNNVKEIHEK FWKYEWKQDI 310 320 330 340 350 360 TLRLMLRSQT TATTARVLAS RPKAPQKVFT LGKVFRPDAI DATHLIEFHQ LDGVIIDNNF 370 380 390 400 410 420 TFKELLGVLK EIFYRLGIKE VKFKPAYFPF TEPSVEAYGY LEKLGWVEMC GAGLLRPEIL 430 440 450 460 SSVGIDSTAG AWGIGIERLA MSFLNISDIR LLYSNNIEYI RDTKVKIE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2." Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y., Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F., Ragan M.A., Charlebois R.L. Mol. Microbiol. 22:175-191(1996) [PubMed: 8899719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08257 Genomic DNA. Translation: CAA69551.1. AE006641 Genomic DNA. Translation: AAK40456.1.
PIR	S75389.
RefSeq	NP_341666.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P95961.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1453284.
GenomeReviews	Gene locus SSO0100 in contig AE006641_GR.
KEGG	sso:SSO0100.
NMPDR	fig|273057.1.peg.89.
Phylogenomic databases
HOGENOM	HBG497668.
OMA	PEFYQCE.
ProtClustDB	PRK04172.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO0100-MONOMER.
Family and domain databases
HAMAP	MF_00282. Phe_tRNA_synth_alpha2. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR022917. Phe_tRNA_synth_alpha2_bac/arc. IPR002319. Phenylalanyl-tRNA_Synthase. IPR002831. Tscrpt_reg_TrmB_N. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01889.
Pfam	PF01978. TrmB. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_SULSO

Accession

Primary (citable) accession number: P95961

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program