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Protein

Leucyl aminopeptidase

Gene

ape2

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially acts as a leucyl-aminopeptidase, although it also has activity against other substrates.

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

pH dependencei

Optimum pH is 6.

Temperature dependencei

Optimum temperature is 80 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei106 – 1061SubstrateBy similarity
Metal bindingi273 – 2731Zinc; catalyticPROSITE-ProRule annotation
Active sitei274 – 2741Proton acceptorPROSITE-ProRule annotation
Metal bindingi277 – 2771Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi296 – 2961Zinc; catalyticPROSITE-ProRule annotation
Sitei359 – 3591Transition state stabilizerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2015-MONOMER.
BRENDAi3.4.11.B3. 6163.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucyl aminopeptidase (EC:3.4.11.1)
Gene namesi
Name:ape2
Synonyms:lap
Ordered Locus Names:SSO2154
ORF Names:C01_030
OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifieri273057 [NCBI]
Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
Proteomesi
  • UP000001974 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 785785Leucyl aminopeptidasePRO_0000095107Add
BLAST

Post-translational modificationi

Can be phosphorylated by cell extracts.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Co-immunoprecipitates with the 60 kDa chaperonin.

Protein-protein interaction databases

STRINGi273057.SSO2154.

Structurei

3D structure databases

ProteinModelPortaliP95928.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2425Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiarCOG02969. Archaea.
COG0308. LUCA.
HOGENOMiHOG000106482.
InParanoidiP95928.
KOiK13722.
OMAiITFREIY.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P95928-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKVNRYEIF LDFSFQTGDY KGYEKIEMES DEETVVLDAV GLKIVKAKVN
60 70 80 90 100
GKEIEFSQDE SRVNVKSGSF SGILEVEFEG KVTERKLVGI YKASYKDGYV
110 120 130 140 150
ISTQFEATHA RDFIPCFDHP AMKARFKLTV RVDKGLKVIS NMPVVREKEE
160 170 180 190 200
NGKVVYEFDE TPKMSTYLLY LGIGNFEEIR DEGKIPTIIV ATIPGKVQKG
210 220 230 240 250
RFSMQISRNS IEFYEKYFEI PYQLPKVHLI AIPEFAYGAM ENWGAITFRE
260 270 280 290 300
TALLADDSSS VYQKFRVAEV VAHELAHQWF GNLVTLKWWD DLWLNESFAT
310 320 330 340 350
FMSHKAISQL FPSWNFWDYF VLNQTSRALE KDSVSTTHPI EAHVRDPNEV
360 370 380 390 400
EQMFDDISYG KGASILRMIE AYVGEENFRR GVVNYLKKFS YSNAQGSDLW
410 420 430 440 450
NSISEVYGSD ISPIMADWIT KPGYPMVRVS VSGKRVSLEQ ERFSLIGNVE
460 470 480 490 500
NLLYKIPLTM EVNGKVVTHL LDKERDTMVF EEDVKSLKVN VNRTGFYRVF
510 520 530 540 550
YYNNSDLVFN SNLSELDKWG IINDYWAFLL AGKIGFKEYE RVISKFFNDK
560 570 580 590 600
DFLPVNELSN ELFTLHAINP DKYQGIAKEF HRIQLKNWRN SKDELGRLTY
610 620 630 640 650
SNILYRLAAI DDEFSLGLSE LFRFYGSLDS DTRQGVAVAY AITYEDNSVD
660 670 680 690 700
ELLERFRKET FDEEKLRYLT AMLFFRKPYL VGNTLSLILS GEIKKQDIPL
710 720 730 740 750
TLSTAAYNPY AKSAVLNWIK MHINFMREAY KGTGILGRRL AEVIPLIGIG
760 770 780
AERETEQFFS NLNMPEAERG IGTGLELLKA YSRLK
Length:785
Mass (Da):90,515
Last modified:May 1, 1997 - v1
Checksum:i73ACFDEB1F2832BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043465 Genomic DNA. Translation: AAC63218.1.
Y08256 Genomic DNA. Translation: CAA69432.1.
AE006641 Genomic DNA. Translation: AAK42330.1.
PIRiS73098.
RefSeqiWP_009992163.1. NC_002754.1.

Genome annotation databases

EnsemblBacteriaiAAK42330; AAK42330; SSO2154.
GeneIDi27428475.
KEGGisso:SSO2154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043465 Genomic DNA. Translation: AAC63218.1.
Y08256 Genomic DNA. Translation: CAA69432.1.
AE006641 Genomic DNA. Translation: AAK42330.1.
PIRiS73098.
RefSeqiWP_009992163.1. NC_002754.1.

3D structure databases

ProteinModelPortaliP95928.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi273057.SSO2154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK42330; AAK42330; SSO2154.
GeneIDi27428475.
KEGGisso:SSO2154.

Phylogenomic databases

eggNOGiarCOG02969. Archaea.
COG0308. LUCA.
HOGENOMiHOG000106482.
InParanoidiP95928.
KOiK13722.
OMAiITFREIY.

Enzyme and pathway databases

BioCyciSSOL273057:GCH2-2015-MONOMER.
BRENDAi3.4.11.B3. 6163.

Family and domain databases

InterProiIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PfamiPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPE2_SULSO
AccessioniPrimary (citable) accession number: P95928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.