P95872 (RIB7_SULSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase
Short name=DAROPP reductase
Short name=DARP reductase
EC=1.1.1.302

Alternative name(s):
2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one reductase
2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate synthase
Short name=DARIPP synthase

Gene names

Ordered Locus Names:	SSO2097
ORF Names:	C06025, C06_008

Organism

Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) [Reference proteome] [HAMAP]

Taxonomic identifier

273057 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus › Sulfolobus solfataricus

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an early step in riboflavin biosynthesis, the NADPH-dependent reduction of the ribose side chain of 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate, yielding 2,5-diamino-6-ribitylamino-4(3H)-pyrimidinone 5'-phosphate By similarity.
Catalytic activity	2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NAD(P)+ = 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NAD(P)H.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HTP reductase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	5-amino-6-(5-phosphoribosylamino)uracil reductase activity Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase		PRO_0000135946
Regions
	Nucleotide binding	79 – 82	4	NADP By similarity
	Nucleotide binding	145 – 148	4	NADP By similarity
Sites
	Binding site	53	1	NADP By similarity
	Binding site	57	1	NADP By similarity
	Binding site	122	1	NADP; via amide nitrogen and carbonyl oxygen

Sequences

	Sequence		Length	Mass (Da)
	P95872 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: A741F6CADEF7631C	FASTA	213	24,561
	10 20 30 40 50 60 MVMKPYVIIF STVSIDGRLA TKTGYSELSC PYDKQRQHEI RSEVDAVMVG ANTVRVDNPS 70 80 90 100 110 120 LTVKYGKNRR NPIRVVVTRS FNLDPSYKIF TTPPSTVIYT SNYESEKVEE FIRKGVIVRK 130 140 150 160 170 180 FLHLDDLLED LYDNFNVRRL MVEGGGHLIW WFIKDNLYDE IRITISPRIF GNGVSFTQGE 190 200 210 GFIGEDSPRL ELIDAKICEC GNEVHLTYKK YMT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Organizational characteristics and information content of an archaeal genome: 156 kb of sequence from Sulfolobus solfataricus P2." Sensen C.W., Klenk H.-P., Singh R.K., Allard G., Chan C.C.-Y., Liu Q.Y., Penny S.L., Young F., Schenk M.E., Gaasterland T., Doolittle W.F., Ragan M.A., Charlebois R.L. Mol. Microbiol. 22:175-191(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08256 Genomic DNA. Translation: CAA69508.1. AE006641 Genomic DNA. Translation: AAK42277.1.
PIR	S74011.
RefSeq	NP_343487.1. NC_002754.1.
3D structure databases
ProteinModelPortal	P95872.
ModBase	Search...
Protein-protein interaction databases
STRING	273057.SSO2097.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK42277; AAK42277; SSO2097.
GeneID	1453604.
KEGG	sso:SSO2097.
Phylogenomic databases
eggNOG	COG1985.
HOGENOM	HOG000225965.
KO	K14654.
OMA	HLITEWE.
ProtClustDB	PRK05625.
Enzyme and pathway databases
UniPathway	UPA00275.
Family and domain databases
Gene3D	3.40.430.10. 1 hit.
InterPro	IPR024072. DHFR-like_dom. IPR006401. Rib_reduct_arc. IPR011549. RibD_C. IPR002734. RibDG_C. [Graphical view]
Pfam	PF01872. RibD_C. 1 hit. [Graphical view]
SUPFAM	SSF53597. SSF53597. 1 hit.
TIGRFAMs	TIGR01508. rib_reduct_arch. 1 hit. TIGR00227. ribD_Cterm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIB7_SULSO

Accession

Primary (citable) accession number: P95872

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program