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Protein

Malto-oligosyltrehalose trehalohydrolase

Gene

treZ

Organism
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.1 Publication

Kineticsi

  1. KM=7.22 mM for maltotriosyltrehalose1 Publication
  2. KM=5.66 mM for maltotetraosyltrehalose1 Publication
  3. KM=5.89 mM for maltopentaosyltrehalose1 Publication
  4. KM=5.86 mM for maltopentaose1 Publication
  5. KM=5.63 mM for maltohexaose1 Publication
  6. KM=2.63 mM for maltoheptaose1 Publication

    pH dependencei

    Optimum pH is 5.0.1 Publication

    Temperature dependencei

    Optimum temperature is 85 degrees Celsius.1 Publication

    Pathwayi: trehalose biosynthesis

    This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.1 Publication
    View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei255Nucleophile1 Publication1
    Active sitei286Proton donor1 Publication1
    Sitei380Transition state stabilizer1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase
    Biological processCarbohydrate metabolism

    Enzyme and pathway databases

    BRENDAi3.2.1.141. 6163.
    UniPathwayiUPA00299.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malto-oligosyltrehalose trehalohydrolase (EC:3.2.1.1411 Publication)
    Short name:
    MTHase
    Alternative name(s):
    4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
    Maltooligosyl trehalose trehalohydrolase
    Gene namesi
    Name:treZ
    Ordered Locus Names:SSO2093
    OrganismiSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
    Taxonomic identifieri273057 [NCBI]
    Taxonomic lineageiArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus
    Proteomesi
    • UP000001974 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi218W → F or A: 15-fold and 150-fold reduction in specific activity, respectively. 1 Publication1
    Mutagenesisi255D → A: 660-fold reduction in specific activity. 1 Publication1
    Mutagenesisi259A → S: Insignificant change in specific activity. 1 Publication1
    Mutagenesisi286E → A: 1100-fold reduction in specific activity. 1 Publication1
    Mutagenesisi328Y → F: 2-fold reduction in specific activity. 1 Publication1
    Mutagenesisi355F → Y: Very small decrease in specific activity. 1 Publication1
    Mutagenesisi356R → K: 2-fold reduction in specific activity. 1 Publication1
    Mutagenesisi380D → A: 8140-fold reduction in specific activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003937521 – 561Malto-oligosyltrehalose trehalohydrolaseAdd BLAST561

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi273057.SSO2093.

    Structurei

    3D structure databases

    ProteinModelPortaliP95867.
    SMRiP95867.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni253 – 258Substrate bindingBy similarity6
    Regioni311 – 315Substrate bindingBy similarity5
    Regioni379 – 384Substrate bindingBy similarity6

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family.Curated

    Phylogenomic databases

    eggNOGiarCOG02951. Archaea.
    COG0296. LUCA.
    HOGENOMiHOG000155669.
    InParanoidiP95867.
    KOiK01236.
    OMAiFTPMLFM.
    OrthoDBiPOG093Z00WH.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiView protein in InterPro
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    IPR015156. Maltooligo_trehalose_arc_C.
    IPR012768. Trehalose_TreZ.
    PfamiView protein in Pfam
    PF09071. Alpha-amyl_C. 1 hit.
    PF00128. Alpha-amylase. 2 hits.
    PF02922. CBM_48. 1 hit.
    PIRSFiPIRSF006337. Trehalose_TreZ. 1 hit.
    SMARTiView protein in SMART
    SM00642. Aamy. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR02402. trehalose_TreZ. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P95867-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTFGYKLDED GVTFNLWAPY QRKVKLKILN RGIYEMERDD KGYFTITLDN
    60 70 80 90 100
    VRVGDRYKYI LDDNSEVPDP ASRYQPEGVH GYSEIISPDF EWDDENSVKV
    110 120 130 140 150
    KREDLVIYEL HIGTFTSEGT FEGVIKKLNY LKELGVTAIE IMPIAQFPGK
    160 170 180 190 200
    KDWGYDGVYL YAVQNSYGGP SGFRKLVNEA HKLGLAVILD VVYNHVGPEG
    210 220 230 240 250
    NYMVKLGPYF SEKYKTPWGL TFNFDDAGSD EVRKFILENV EYWINEFHVD
    260 270 280 290 300
    GFRLDAVHAI IDNSPKHILE DIADVVHKYD KIVIAESDLN DPRVVNPKEK
    310 320 330 340 350
    CGYNIDAQWV DDFHHAIHAF LTGERQGYYS DFGSIGDIVK SYKDVFIYDG
    360 370 380 390 400
    KYSNFRRKTH GKSVGDLDGC KFVVYIQNHD QVGNRGGGER LIKLVDKESY
    410 420 430 440 450
    KIAAALYILS PYIPMIFMGE EYGEENPFYY FSDFSDPKLI QGVREGRRRE
    460 470 480 490 500
    NGQETDPQSD CTFNDSKLSW KINDDILSFY KSLIKIRKEY GLACNRKLSV
    510 520 530 540 550
    ENGNYWLTVK GNGCLAVYVF SKSVIEMKYS GTLVLSSNSS FPSQITESKY
    560
    ELDKGFALYK L
    Length:561
    Mass (Da):64,371
    Last modified:May 1, 1997 - v1
    Checksum:iB00E403020F6B242
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y08256 Genomic DNA. Translation: CAA69503.1.
    AE006641 Genomic DNA. Translation: AAK42272.1.
    PIRiS73087.
    RefSeqiWP_009989788.1. NC_002754.1.

    Genome annotation databases

    EnsemblBacteriaiAAK42272; AAK42272; SSO2093.
    GeneIDi27428413.
    KEGGisso:SSO2093.
    PATRICifig|273057.12.peg.2171.

    Similar proteinsi

    Entry informationi

    Entry nameiTREZ_SULSO
    AccessioniPrimary (citable) accession number: P95867
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: May 1, 1997
    Last modified: August 30, 2017
    This is version 122 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families