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P95803 (DDL_STRMU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:SMU_599
OrganismStreptococcus mutans serotype c (strain ATCC 700610 / UA159) [Complete proteome] [HAMAP]
Taxonomic identifier210007 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177886

Regions

Domain133 – 335203ATP-grasp
Nucleotide binding163 – 21856ATP By similarity

Sites

Metal binding2891Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 1 By similarity
Metal binding3021Magnesium or manganese 2 By similarity
Metal binding3041Magnesium or manganese 2 By similarity

Experimental info

Sequence conflict1321N → K in AAB41854. Ref.2
Sequence conflict2381D → V in AAB41854. Ref.2
Sequence conflict2731A → R in AAB41854. Ref.2

Secondary structure

..................................................... 349
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P95803 [UniParc].

Last modified November 28, 2002. Version 2.
Checksum: FFDAAD9942CAE7EF

FASTA34938,838
        10         20         30         40         50         60 
MSKETLVLLY GGRSAERDVS VLSAESVMRA INYDNFLVKT YFITQAGDFI KTQEFDSQPS 

        70         80         90        100        110        120 
ETDKLMTNDT IIASQKIKPS DIYEEEAVVF PVLHGPMGED GSIQGFLEVL KMPYVGTNIL 

       130        140        150        160        170        180 
SSSVAMDKIT TNQVLESATT IPQVAYVALI EGEPLESKLA EVEEKLIYPV FVKPANMGSS 

       190        200        210        220        230        240 
VGISKAENRT DLKQAIALAL KYDSRVLIEQ GVDAREIEVG ILGNTDVKTT LPGEIVKDVA 

       250        260        270        280        290        300 
FYDYEAKYID NKITMAIPAE IDPVIVEKMR DYAATAFRTL GCCGLSRCDF FLTEDGKVYL 

       310        320        330        340 
NELNTMPGFT QWSMYPLLWE NMGLSYSVLI EELVSLAKEM FDKRESHLV 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014133 Genomic DNA. Translation: AAN58338.1.
U69165 Genomic DNA. Translation: AAB41854.1.
RefSeqNP_721032.1. NC_004350.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3K3PX-ray2.23A/B1-349[»]
ProteinModelPortalP95803.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING210007.SMU.599.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN58338; AAN58338; SMU_599.
GeneID1029384.
KEGGsmu:SMU_599.
PATRIC19663399. VBIStrMut61772_0532.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycSMUT210007:GC7Z-588-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP95803.

Entry information

Entry nameDDL_STRMU
AccessionPrimary (citable) accession number: P95803
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 28, 2002
Last modified: February 19, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways