P95748 (P95748_STRFR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
N-methyltransferase EMBL CAA57473.2

Gene names

Name:

tylMI(orf3*) EMBL CAA57473.2

Organism

Streptomyces fradiae (Streptomyces roseoflavus) EMBL CAA57473.2

Taxonomic identifier

1906 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine PDB 3PFG
Molecular function	Methyltransferase EMBL CAA57473.2 Transferase
Technical term	3D-structure PDB 3PFG PDB 3PFH PDB 3PX2 PDB 3PX3
Gene Ontology (GO)
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	58 – 59	2	S-adenosyl-L-methionine binding PDB 3PFG
	Region	101 – 102	2	S-adenosyl-L-homocysteine binding PDB 3PFH PDB 3PX2 PDB 3PX3
	Region	101 – 102	2	S-adenosyl-L-methionine binding PDB 3PFG
Sites
	Binding site	14	1	S-adenosyl-L-homocysteine PDB 3PFH PDB 3PX2 PDB 3PX3
	Binding site	14	1	S-adenosyl-L-methionine PDB 3PFG
	Binding site	22	1	S-adenosyl-L-homocysteine PDB 3PFH PDB 3PX2 PDB 3PX3
	Binding site	22	1	S-adenosyl-L-methionine PDB 3PFG
	Binding site	33	1	S-adenosyl-L-homocysteine PDB 3PFH PDB 3PX2 PDB 3PX3
	Binding site	33	1	S-adenosyl-L-methionine PDB 3PFG
	Binding site	58	1	S-adenosyl-L-homocysteine; via carbonyl oxygen PDB 3PFH PDB 3PX2 PDB 3PX3
	Binding site	79	1	S-adenosyl-L-homocysteine PDB 3PFH PDB 3PX2 PDB 3PX3
	Binding site	79	1	S-adenosyl-L-methionine PDB 3PFG
	Binding site	117	1	S-adenosyl-L-homocysteine; via carbonyl oxygen PDB 3PFH
	Binding site	117	1	S-adenosyl-L-methionine; via carbonyl oxygen PDB 3PFG

Sequences

	Sequence		Length	Mass (Da)
	P95748 [UniParc]. Last modified November 1, 1999. Version 2. Checksum: 74C1034DA07FA07A	FASTA	255	27,428
	10 20 30 40 50 60 MAHSSATAGP QADYSGEIAE LYDLVHQGKG KDYHREAADL AALVRRHSPK AASLLDVACG 70 80 90 100 110 120 TGMHLRHLAD SFGTVEGLEL SADMLAIARR RNPDAVLHHG DMRDFSLGRR FSAVTCMFSS 130 140 150 160 170 180 IGHLAGQAEL DAALERFAAH VLPDGVVVVE PWWFPENFTP GYVAAGTVEA GGTTVTRVSH 190 200 210 220 230 240 SSREGEATRI EVHYLVAGPD RGITHHEESH RITLFTREQY ERAFTAAGLS VEFMPGGPSG 250 RGLFTGLPGA KGETR

References

[1]	"Analysis of four tylosin biosynthetic genes from the tylLM region of Streptomyces fradiae." Gandecha A.R., Large S.L., Cundliffe E. Gene 184:197-203(1997) [PubMed: 9031628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: T59235 EMBL CAA57473.2.
[2]	Gandecha A.R. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: T59235 EMBL CAA57473.2.
[3]	"Molecular architecture of TylM1 from Streptomyces fradiae: an N,N-dimethyltransferase involved in the production of dTDP-D-mycaminose." Carney A.E., Holden H.M. Biochemistry 50:780-787(2011) [PubMed: 21142177] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) IN COMPLEX WITH S-ADENOSYL-L-HOMOCYSTEINE AND S-ADENOSYL-L-METHIONINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X81885 Genomic DNA. Translation: CAA57473.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PFG	X-ray	1.35	A	1-255	[»]
3PFH	X-ray	1.79	A/D	1-255	[»]
3PX2	X-ray	1.65	A/D	1-255	[»]
3PX3	X-ray	1.80	A/D	1-255	[»]

ProteinModelPortal

P95748.

SMR

P95748. Positions 14-249.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

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Family and domain databases

ProtoNet

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Entry information

Entry name

P95748_STRFR

Accession

Primary (citable) accession number: P95748

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 1997
Last sequence update:	November 1, 1999
Last modified:	October 19, 2011
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)