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Protein

GTPase Obg

Gene

obg

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an unknown essential role and a regulatory role in sporulation. Overexpression suppresses sporulation although cell growth rate was not reduced. Impaired differentiation was eliminated by addition of decoyinine, an inhibitor of GMP synthesis. Overexpression has no effect on undecylprodigiosin production, but decreases actinorhodin production.1 Publication
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. Plays a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi173MagnesiumUniRule annotation1
Metal bindingi193MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 173GTPUniRule annotation8
Nucleotide bindingi191 – 195GTPUniRule annotation5
Nucleotide bindingi212 – 215GTPUniRule annotation4
Nucleotide bindingi282 – 285GTPUniRule annotation4
Nucleotide bindingi311 – 313GTPUniRule annotation3

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase ObgUniRule annotation (EC:3.6.5.-UniRule annotation)
Alternative name(s):
GTP-binding protein ObgUniRule annotation
Gene namesi
Name:obgUniRule annotation
Ordered Locus Names:SCO2595
ORF Names:SCC88.06c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication
  • Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
  • Note: No longer associated with the cell membrane after salt-washing.

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it cannot be disrupted.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi168P → V: Overexpression suppresses sporulation more than the wild-type gene. 1 Publication1
Mutagenesisi171G → A: Overexpression enhances aerial mycelium formation, also enhances actinorhodin production. 1 Publication1
Mutagenesisi172K → N: No phenotype upon overexpression. 1 Publication1
Mutagenesisi173S → N: No phenotype upon overexpression. 1 Publication1
Mutagenesisi281L → I: No phenotype upon overexpression. 1 Publication1
Mutagenesisi285D → A: No phenotype upon overexpression. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003863231 – 478GTPase ObgAdd BLAST478

Expressioni

Inductioni

Expressed during vegetative growth it disappears during stationary phase and sporulation (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO2595

Structurei

3D structure databases

ProteinModelPortaliP95722
SMRiP95722
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 330OBG-type GUniRule annotationAdd BLAST171

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9R Bacteria
COG0536 LUCA
HOGENOMiHOG000019083
InParanoidiP95722
KOiK03979
OMAiPHVGIVH
OrthoDBiPOG091H025M
PhylomeDBiP95722

Family and domain databases

CDDicd01898 Obg, 1 hit
Gene3Di2.40.240.60, 1 hit
2.70.210.12, 1 hit
HAMAPiMF_01454 GTPase_Obg, 1 hit
InterProiView protein in InterPro
IPR031167 G_OBG
IPR014100 GTP-bd_Obg/CgtA
IPR015349 GTP-bd_prot_GTP1/OBG_C
IPR036346 GTP-bd_prot_GTP1/OBG_C_sf
IPR006074 GTP1-OBG_CS
IPR006169 GTP1_OBG_dom
IPR036726 GTP1_OBG_dom_sf
IPR006073 GTP_binding_domain
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF09269 DUF1967, 1 hit
PF01018 GTP1_OBG, 1 hit
PF01926 MMR_HSR1, 1 hit
PRINTSiPR00326 GTP1OBG
SUPFAMiSSF102741 SSF102741, 1 hit
SSF52540 SSF52540, 1 hit
SSF82051 SSF82051, 1 hit
TIGRFAMsiTIGR02729 Obg_CgtA, 1 hit
TIGR03595 Obg_CgtA_exten, 1 hit
PROSITEiView protein in PROSITE
PS51710 G_OBG, 1 hit
PS00905 GTP1_OBG, 1 hit

Sequencei

Sequence statusi: Complete.

P95722-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTFVDRVEL HVAAGNGGHG CASVHREKFK PLGGPDGGNG GRGGDVILTV
60 70 80 90 100
DQSVTTLLDY HHSPHRKATN GKPGEGGNRS GKDGQDLVLP VPDGTVVLDG
110 120 130 140 150
AGNVLADLVG HGTSYVAAQG GRGGLGNAAL ASARRKAPGF ALLGEPGDLQ
160 170 180 190 200
DIHLELKTVA DVALVGYPSA GKSSLISVLS AAKPKIADYP FTTLVPNLGV
210 220 230 240 250
VTAGETVYTV ADVPGLIPGA SQGKGLGLEF LRHVERCSVL VHVLDTATLE
260 270 280 290 300
SERDPLSDLD VIETELREYG GLDNRPRIVV LNKIDVPDGK DLAEMVRPDL
310 320 330 340 350
EARGYRVFEV SAVAHMGLRE LSFALAELVA TARAARPKEE ATRIVIRPKA
360 370 380 390 400
VDDAGFTVTR EEDGLFRVRG EKPERWVRQT DFNNDEAVGY LSDRLNRLGV
410 420 430 440 450
EDKLMKAGAR NGDGVAIGPE DNAVVFDWEP SVTAGAEMLG RRGEDHRFEA
460 470
PRPAAQRRRD RDAERDEAQQ EFDGFEPF
Length:478
Mass (Da):51,073
Last modified:May 1, 1997 - v1
Checksum:iACAFBCDCF4C9E53B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87915 Genomic DNA Translation: BAA13498.1
AL939113 Genomic DNA Translation: CAB75376.1
PIRiT42036
RefSeqiNP_626832.1, NC_003888.3
WP_003976206.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAB75376; CAB75376; CAB75376
GeneIDi1098029
KEGGisco:SCO2595
PATRICifig|100226.15.peg.2641

Similar proteinsi

Entry informationi

Entry nameiOBG_STRCO
AccessioniPrimary (citable) accession number: P95722
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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