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P95722 (OBG_STRCO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase Obg
Alternative name(s):
GTP-binding protein Obg
Gene names
Name:obg
Ordered Locus Names:SCO2595
ORF Names:SCC88.06c
OrganismStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]
Taxonomic identifier100226 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Protein attributes

Sequence length478 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an unknown essential role and a regulatory role in sporulation. Overexpression suppresses sporulation although cell growth rate was not reduced. Impaired differentiation was eliminated by addition of decoyinine, an inhibitor of GMP synthesis. Overexpression has no effect on undecylprodigiosin production, but decreases actinorhodin production. HAMAP-Rule MF_01454

An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate By similarity. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control Potential. HAMAP-Rule MF_01454

Cofactor

Magnesium Potential.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01454

Subcellular location

Cytoplasm. Cell membrane; Peripheral membrane protein. Note: No longer associated with the cell membrane after salt-washing. Ref.3

Induction

Expressed during vegetative growth it disappears during stationary phase and sporulation (at protein level). Ref.3

Disruption phenotype

Essential for growth, it cannot be disrupted. Ref.3

Sequence similarities

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.

Contains 1 OBG-type G (guanine nucleotide-binding) domain.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Membrane
   LigandGTP-binding
Nucleotide-binding
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 478478GTPase Obg HAMAP-Rule MF_01454
PRO_0000386323

Regions

Domain160 – 330171OBG-type G
Nucleotide binding166 – 1738GTP By similarity
Nucleotide binding212 – 2165GTP By similarity
Nucleotide binding282 – 2854GTP By similarity

Experimental info

Mutagenesis1681P → V: Overexpression suppresses sporulation more than the wild-type gene. Ref.3
Mutagenesis1711G → A: Overexpression enhances aerial mycelium formation, also enhances actinorhodin production. Ref.3
Mutagenesis1721K → N: No phenotype upon overexpression. Ref.3
Mutagenesis1731S → N: No phenotype upon overexpression. Ref.3
Mutagenesis2811L → I: No phenotype upon overexpression. Ref.3
Mutagenesis2851D → A: No phenotype upon overexpression. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P95722 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: ACAFBCDCF4C9E53B

FASTA47851,073
        10         20         30         40         50         60 
MTTFVDRVEL HVAAGNGGHG CASVHREKFK PLGGPDGGNG GRGGDVILTV DQSVTTLLDY 

        70         80         90        100        110        120 
HHSPHRKATN GKPGEGGNRS GKDGQDLVLP VPDGTVVLDG AGNVLADLVG HGTSYVAAQG 

       130        140        150        160        170        180 
GRGGLGNAAL ASARRKAPGF ALLGEPGDLQ DIHLELKTVA DVALVGYPSA GKSSLISVLS 

       190        200        210        220        230        240 
AAKPKIADYP FTTLVPNLGV VTAGETVYTV ADVPGLIPGA SQGKGLGLEF LRHVERCSVL 

       250        260        270        280        290        300 
VHVLDTATLE SERDPLSDLD VIETELREYG GLDNRPRIVV LNKIDVPDGK DLAEMVRPDL 

       310        320        330        340        350        360 
EARGYRVFEV SAVAHMGLRE LSFALAELVA TARAARPKEE ATRIVIRPKA VDDAGFTVTR 

       370        380        390        400        410        420 
EEDGLFRVRG EKPERWVRQT DFNNDEAVGY LSDRLNRLGV EDKLMKAGAR NGDGVAIGPE 

       430        440        450        460        470 
DNAVVFDWEP SVTAGAEMLG RRGEDHRFEA PRPAAQRRRD RDAERDEAQQ EFDGFEPF 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation."
Okamoto S., Itoh M., Ochi K.
J. Bacteriol. 179:170-179(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC BAA-471 / A3(2) / M145.
[2]"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)."
Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. expand/collapse author list , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.
Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-471 / A3(2) / M145.
[3]"An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor."
Okamoto S., Ochi K.
Mol. Microbiol. 30:107-119(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF PRO-168; GLY-171; LYS-172; SER-173; LEU-281 AND ASP-285, INDUCTION, SUBCELLULAR LOCATION, OVEREXPRESSION, DISRUPTION PHENOTYPE.
[4]"Obg/CtgA, a signaling protein that controls replication, translation, and morphological development?"
Michel B.
Dev. Cell 8:300-301(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87915 Genomic DNA. Translation: BAA13498.1.
AL939113 Genomic DNA. Translation: CAB75376.1.
PIRT42036.
RefSeqNP_626832.1. NC_003888.3.

3D structure databases

ProteinModelPortalP95722.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING100226.SCO2595.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB75376; CAB75376; CAB75376.
GeneID1098029.
KEGGsco:SCO2595.
PATRIC23734876. VBIStrCoe124346_2641.

Phylogenomic databases

eggNOGCOG0536.
HOGENOMHOG000019083.
KOK03979.
OMAVFDWEPT.
OrthoDBEOG6H1Q1M.
PhylomeDBP95722.
ProtClustDBPRK12296.

Family and domain databases

Gene3D2.70.210.12. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_01454. GTPase_Obg.
InterProIPR014100. GTP-bd_Obg/CgtA.
IPR015349. GTP-bd_prot_GTP1/OBG_C.
IPR006074. GTP1-OBG_CS.
IPR006169. GTP1_OBG_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR11702:SF3. PTHR11702:SF3. 1 hit.
PfamPF09269. DUF1967. 1 hit.
PF01018. GTP1_OBG. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
PIRSFPIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTSPR00326. GTP1OBG.
SUPFAMSSF102741. SSF102741. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF82051. SSF82051. 1 hit.
TIGRFAMsTIGR02729. Obg_CgtA. 1 hit.
TIGR03595. Obg_CgtA_exten. 1 hit.
PROSITEPS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOBG_STRCO
AccessionPrimary (citable) accession number: P95722
Entry history
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families