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P95722

- OBG_STRCO

UniProt

P95722 - OBG_STRCO

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Protein
GTPase Obg
Gene
obg, SCO2595, SCC88.06c
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays an unknown essential role and a regulatory role in sporulation. Overexpression suppresses sporulation although cell growth rate was not reduced. Impaired differentiation was eliminated by addition of decoyinine, an inhibitor of GMP synthesis. Overexpression has no effect on undecylprodigiosin production, but decreases actinorhodin production.UniRule annotation
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate By similarity. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control Reviewed prediction.UniRule annotation

Cofactori

Magnesium Reviewed prediction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi166 – 1738GTP By similarity
Nucleotide bindingi212 – 2165GTP By similarity
Nucleotide bindingi282 – 2854GTP By similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-HAMAP
  2. GTPase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase Obg
Alternative name(s):
GTP-binding protein Obg
Gene namesi
Name:obg
Ordered Locus Names:SCO2595
ORF Names:SCC88.06c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm. Cell membrane; Peripheral membrane protein
Note: No longer associated with the cell membrane after salt-washing.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it cannot be disrupted.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi168 – 1681P → V: Overexpression suppresses sporulation more than the wild-type gene. 1 Publication
Mutagenesisi171 – 1711G → A: Overexpression enhances aerial mycelium formation, also enhances actinorhodin production. 1 Publication
Mutagenesisi172 – 1721K → N: No phenotype upon overexpression. 1 Publication
Mutagenesisi173 – 1731S → N: No phenotype upon overexpression. 1 Publication
Mutagenesisi281 – 2811L → I: No phenotype upon overexpression. 1 Publication
Mutagenesisi285 – 2851D → A: No phenotype upon overexpression. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478GTPase ObgUniRule annotation
PRO_0000386323Add
BLAST

Expressioni

Inductioni

Expressed during vegetative growth it disappears during stationary phase and sporulation (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO2595.

Structurei

3D structure databases

ProteinModelPortaliP95722.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini160 – 330171OBG-type G
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0536.
HOGENOMiHOG000019083.
KOiK03979.
OMAiNGGHGCA.
OrthoDBiEOG6H1Q1M.
PhylomeDBiP95722.

Family and domain databases

Gene3Di2.70.210.12. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01454. GTPase_Obg.
InterProiIPR014100. GTP-bd_Obg/CgtA.
IPR015349. GTP-bd_prot_GTP1/OBG_C.
IPR006074. GTP1-OBG_CS.
IPR006169. GTP1_OBG_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11702:SF3. PTHR11702:SF3. 1 hit.
PfamiPF09269. DUF1967. 1 hit.
PF01018. GTP1_OBG. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
PIRSFiPIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF102741. SSF102741. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF82051. SSF82051. 1 hit.
TIGRFAMsiTIGR02729. Obg_CgtA. 1 hit.
TIGR03595. Obg_CgtA_exten. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P95722-1 [UniParc]FASTAAdd to Basket

« Hide

MTTFVDRVEL HVAAGNGGHG CASVHREKFK PLGGPDGGNG GRGGDVILTV    50
DQSVTTLLDY HHSPHRKATN GKPGEGGNRS GKDGQDLVLP VPDGTVVLDG 100
AGNVLADLVG HGTSYVAAQG GRGGLGNAAL ASARRKAPGF ALLGEPGDLQ 150
DIHLELKTVA DVALVGYPSA GKSSLISVLS AAKPKIADYP FTTLVPNLGV 200
VTAGETVYTV ADVPGLIPGA SQGKGLGLEF LRHVERCSVL VHVLDTATLE 250
SERDPLSDLD VIETELREYG GLDNRPRIVV LNKIDVPDGK DLAEMVRPDL 300
EARGYRVFEV SAVAHMGLRE LSFALAELVA TARAARPKEE ATRIVIRPKA 350
VDDAGFTVTR EEDGLFRVRG EKPERWVRQT DFNNDEAVGY LSDRLNRLGV 400
EDKLMKAGAR NGDGVAIGPE DNAVVFDWEP SVTAGAEMLG RRGEDHRFEA 450
PRPAAQRRRD RDAERDEAQQ EFDGFEPF 478
Length:478
Mass (Da):51,073
Last modified:May 1, 1997 - v1
Checksum:iACAFBCDCF4C9E53B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87915 Genomic DNA. Translation: BAA13498.1.
AL939113 Genomic DNA. Translation: CAB75376.1.
PIRiT42036.
RefSeqiNP_626832.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB75376; CAB75376; CAB75376.
GeneIDi1098029.
KEGGisco:SCO2595.
PATRICi23734876. VBIStrCoe124346_2641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D87915 Genomic DNA. Translation: BAA13498.1 .
AL939113 Genomic DNA. Translation: CAB75376.1 .
PIRi T42036.
RefSeqi NP_626832.1. NC_003888.3.

3D structure databases

ProteinModelPortali P95722.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO2595.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB75376 ; CAB75376 ; CAB75376 .
GeneIDi 1098029.
KEGGi sco:SCO2595.
PATRICi 23734876. VBIStrCoe124346_2641.

Phylogenomic databases

eggNOGi COG0536.
HOGENOMi HOG000019083.
KOi K03979.
OMAi NGGHGCA.
OrthoDBi EOG6H1Q1M.
PhylomeDBi P95722.

Family and domain databases

Gene3Di 2.70.210.12. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_01454. GTPase_Obg.
InterProi IPR014100. GTP-bd_Obg/CgtA.
IPR015349. GTP-bd_prot_GTP1/OBG_C.
IPR006074. GTP1-OBG_CS.
IPR006169. GTP1_OBG_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11702:SF3. PTHR11702:SF3. 1 hit.
Pfami PF09269. DUF1967. 1 hit.
PF01018. GTP1_OBG. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view ]
PIRSFi PIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTSi PR00326. GTP1OBG.
SUPFAMi SSF102741. SSF102741. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF82051. SSF82051. 1 hit.
TIGRFAMsi TIGR02729. Obg_CgtA. 1 hit.
TIGR03595. Obg_CgtA_exten. 1 hit.
PROSITEi PS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation."
    Okamoto S., Itoh M., Ochi K.
    J. Bacteriol. 179:170-179(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor."
    Okamoto S., Ochi K.
    Mol. Microbiol. 30:107-119(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PRO-168; GLY-171; LYS-172; SER-173; LEU-281 AND ASP-285, INDUCTION, SUBCELLULAR LOCATION, OVEREXPRESSION, DISRUPTION PHENOTYPE.
  4. "Obg/CtgA, a signaling protein that controls replication, translation, and morphological development?"
    Michel B.
    Dev. Cell 8:300-301(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiOBG_STRCO
AccessioniPrimary (citable) accession number: P95722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: May 1, 1997
Last modified: May 14, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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