P95722 (OBG_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase obg

Alternative name(s):
GTP-binding protein obg

Gene names

Name:	obg
Ordered Locus Names:	SCO2595
ORF Names:	SCC88.06c

Organism

Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) [Reference proteome] [HAMAP]

Taxonomic identifier

100226 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces › Streptomyces coelicolor

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an unknown essential role and a regulatory role in sporulation. Overexpression suppresses sporulation although cell growth rate was not reduced. Impaired differentiation was eliminated by addition of decoyinine, an inhibitor of GMP synthesis. Overexpression has no effect on undecylprodigiosin production, but decreases actinorhodin production. HAMAP-Rule MF_01454 An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate By similarity. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control Potential. HAMAP-Rule MF_01454
Cofactor	Magnesium Potential.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. Cell membrane; Peripheral membrane protein. Note: No longer associated with the cell membrane after salt-washing. Ref.3
Induction	Expressed during vegetative growth it disappears during stationary phase and sporulation (at protein level). Ref.3
Disruption phenotype	Essential for growth, it cannot be disrupted. Ref.3
Sequence similarities	Belongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	GTPase obg HAMAP-Rule MF_01454		PRO_0000386323
Regions
	Domain	171 – 283	113	G
	Nucleotide binding	166 – 173	8	GTP By similarity
	Nucleotide binding	212 – 216	5	GTP By similarity
	Nucleotide binding	282 – 285	4	GTP By similarity
Experimental info
	Mutagenesis	168	1	P → V: Overexpression suppresses sporulation more than the wild-type gene. Ref.3
	Mutagenesis	171	1	G → A: Overexpression enhances aerial mycelium formation, also enhances actinorhodin production. Ref.3
	Mutagenesis	172	1	K → N: No phenotype upon overexpression. Ref.3
	Mutagenesis	173	1	S → N: No phenotype upon overexpression. Ref.3
	Mutagenesis	281	1	L → I: No phenotype upon overexpression. Ref.3
	Mutagenesis	285	1	D → A: No phenotype upon overexpression. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P95722 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: ACAFBCDCF4C9E53B	FASTA	478	51,073
	10 20 30 40 50 60 MTTFVDRVEL HVAAGNGGHG CASVHREKFK PLGGPDGGNG GRGGDVILTV DQSVTTLLDY 70 80 90 100 110 120 HHSPHRKATN GKPGEGGNRS GKDGQDLVLP VPDGTVVLDG AGNVLADLVG HGTSYVAAQG 130 140 150 160 170 180 GRGGLGNAAL ASARRKAPGF ALLGEPGDLQ DIHLELKTVA DVALVGYPSA GKSSLISVLS 190 200 210 220 230 240 AAKPKIADYP FTTLVPNLGV VTAGETVYTV ADVPGLIPGA SQGKGLGLEF LRHVERCSVL 250 260 270 280 290 300 VHVLDTATLE SERDPLSDLD VIETELREYG GLDNRPRIVV LNKIDVPDGK DLAEMVRPDL 310 320 330 340 350 360 EARGYRVFEV SAVAHMGLRE LSFALAELVA TARAARPKEE ATRIVIRPKA VDDAGFTVTR 370 380 390 400 410 420 EEDGLFRVRG EKPERWVRQT DFNNDEAVGY LSDRLNRLGV EDKLMKAGAR NGDGVAIGPE 430 440 450 460 470 DNAVVFDWEP SVTAGAEMLG RRGEDHRFEA PRPAAQRRRD RDAERDEAQQ EFDGFEPF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation." Okamoto S., Itoh M., Ochi K. J. Bacteriol. 179:170-179(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[2]	"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.
[3]	"An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor." Okamoto S., Ochi K. Mol. Microbiol. 30:107-119(1998) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF PRO-168; GLY-171; LYS-172; SER-173; LEU-281 AND ASP-285, INDUCTION, SUBCELLULAR LOCATION, OVEREXPRESSION, DISRUPTION PHENOTYPE.
[4]	"Obg/CtgA, a signaling protein that controls replication, translation, and morphological development?" Michel B. Dev. Cell 8:300-301(2005) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D87915 Genomic DNA. Translation: BAA13498.1. AL939113 Genomic DNA. Translation: CAB75376.1.
PIR	T42036.
RefSeq	NP_626832.1. NC_003888.3.
3D structure databases
HSSP	HSSP built from PDB template 1LNZ based on UniProtKB P20964.
ProteinModelPortal	P95722.
ModBase	Search...
Protein-protein interaction databases
STRING	100226.SCO2595.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB75376; CAB75376; CAB75376.
GeneID	1098029.
KEGG	sco:SCO2595.
PATRIC	23734876. VBIStrCoe124346_2641.
Phylogenomic databases
eggNOG	COG0536.
HOGENOM	HOG000019083.
KO	K03979.
OMA	FNNDEAV.
ProtClustDB	PRK12296.
Family and domain databases
Gene3D	2.70.210.12. 1 hit.
HAMAP	MF_01454. GTPase_Obg.
InterPro	IPR014100. GTP-bd_Obg/CgtA. IPR015349. GTP-bd_prot_GTP1/OBG_C. IPR006074. GTP1-OBG_CS. IPR006169. GTP1_OBG_dom. IPR006073. GTP_binding_domain. [Graphical view]
PANTHER	PTHR11702:SF3. PTHR11702:SF3. 1 hit.
Pfam	PF09269. DUF1967. 1 hit. PF01018. GTP1_OBG. 1 hit. PF01926. MMR_HSR1. 1 hit. [Graphical view]
PIRSF	PIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTS	PR00326. GTP1OBG.
SUPFAM	SSF102741. GTP-bd_prot_GTP1/OBG_C. 1 hit. SSF82051. GTP1_OBG_sub. 1 hit.
TIGRFAMs	TIGR02729. Obg_CgtA. 1 hit. TIGR03595. Obg_CgtA_exten. 1 hit.
PROSITE	PS00905. GTP1_OBG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OBG_STRCO

Accession

Primary (citable) accession number: P95722

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 13, 2009
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program