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P95722

- OBG_STRCO

UniProt

P95722 - OBG_STRCO

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Protein

GTPase Obg

Gene

obg

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an unknown essential role and a regulatory role in sporulation. Overexpression suppresses sporulation although cell growth rate was not reduced. Impaired differentiation was eliminated by addition of decoyinine, an inhibitor of GMP synthesis. Overexpression has no effect on undecylprodigiosin production, but decreases actinorhodin production.
An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi166 – 1738GTPUniRule annotation
Nucleotide bindingi212 – 2165GTPUniRule annotation
Nucleotide bindingi282 – 2854GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTPase ObgUniRule annotation
Alternative name(s):
GTP-binding protein ObgUniRule annotation
Gene namesi
Name:obgUniRule annotation
Ordered Locus Names:SCO2595
ORF Names:SCC88.06c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm 1 PublicationUniRule annotation. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: No longer associated with the cell membrane after salt-washing.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Essential for growth, it cannot be disrupted.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi168 – 1681P → V: Overexpression suppresses sporulation more than the wild-type gene. 1 Publication
Mutagenesisi171 – 1711G → A: Overexpression enhances aerial mycelium formation, also enhances actinorhodin production. 1 Publication
Mutagenesisi172 – 1721K → N: No phenotype upon overexpression. 1 Publication
Mutagenesisi173 – 1731S → N: No phenotype upon overexpression. 1 Publication
Mutagenesisi281 – 2811L → I: No phenotype upon overexpression. 1 Publication
Mutagenesisi285 – 2851D → A: No phenotype upon overexpression. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478GTPase ObgPRO_0000386323Add
BLAST

Expressioni

Inductioni

Expressed during vegetative growth it disappears during stationary phase and sporulation (at protein level).1 Publication

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO2595.

Structurei

3D structure databases

ProteinModelPortaliP95722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini160 – 330171OBG-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0536.
HOGENOMiHOG000019083.
InParanoidiP95722.
KOiK03979.
OMAiNGGHGCA.
OrthoDBiEOG6H1Q1M.
PhylomeDBiP95722.

Family and domain databases

Gene3Di2.70.210.12. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01454. GTPase_Obg.
InterProiIPR014100. GTP-bd_Obg/CgtA.
IPR015349. GTP-bd_prot_GTP1/OBG_C.
IPR006074. GTP1-OBG_CS.
IPR006169. GTP1_OBG_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11702:SF3. PTHR11702:SF3. 1 hit.
PfamiPF09269. DUF1967. 1 hit.
PF01018. GTP1_OBG. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view]
PIRSFiPIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF102741. SSF102741. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF82051. SSF82051. 1 hit.
TIGRFAMsiTIGR02729. Obg_CgtA. 1 hit.
TIGR03595. Obg_CgtA_exten. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P95722-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTFVDRVEL HVAAGNGGHG CASVHREKFK PLGGPDGGNG GRGGDVILTV
60 70 80 90 100
DQSVTTLLDY HHSPHRKATN GKPGEGGNRS GKDGQDLVLP VPDGTVVLDG
110 120 130 140 150
AGNVLADLVG HGTSYVAAQG GRGGLGNAAL ASARRKAPGF ALLGEPGDLQ
160 170 180 190 200
DIHLELKTVA DVALVGYPSA GKSSLISVLS AAKPKIADYP FTTLVPNLGV
210 220 230 240 250
VTAGETVYTV ADVPGLIPGA SQGKGLGLEF LRHVERCSVL VHVLDTATLE
260 270 280 290 300
SERDPLSDLD VIETELREYG GLDNRPRIVV LNKIDVPDGK DLAEMVRPDL
310 320 330 340 350
EARGYRVFEV SAVAHMGLRE LSFALAELVA TARAARPKEE ATRIVIRPKA
360 370 380 390 400
VDDAGFTVTR EEDGLFRVRG EKPERWVRQT DFNNDEAVGY LSDRLNRLGV
410 420 430 440 450
EDKLMKAGAR NGDGVAIGPE DNAVVFDWEP SVTAGAEMLG RRGEDHRFEA
460 470
PRPAAQRRRD RDAERDEAQQ EFDGFEPF
Length:478
Mass (Da):51,073
Last modified:May 1, 1997 - v1
Checksum:iACAFBCDCF4C9E53B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87915 Genomic DNA. Translation: BAA13498.1.
AL939113 Genomic DNA. Translation: CAB75376.1.
PIRiT42036.
RefSeqiNP_626832.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB75376; CAB75376; CAB75376.
GeneIDi1098029.
KEGGisco:SCO2595.
PATRICi23734876. VBIStrCoe124346_2641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87915 Genomic DNA. Translation: BAA13498.1 .
AL939113 Genomic DNA. Translation: CAB75376.1 .
PIRi T42036.
RefSeqi NP_626832.1. NC_003888.3.

3D structure databases

ProteinModelPortali P95722.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO2595.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB75376 ; CAB75376 ; CAB75376 .
GeneIDi 1098029.
KEGGi sco:SCO2595.
PATRICi 23734876. VBIStrCoe124346_2641.

Phylogenomic databases

eggNOGi COG0536.
HOGENOMi HOG000019083.
InParanoidi P95722.
KOi K03979.
OMAi NGGHGCA.
OrthoDBi EOG6H1Q1M.
PhylomeDBi P95722.

Family and domain databases

Gene3Di 2.70.210.12. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_01454. GTPase_Obg.
InterProi IPR014100. GTP-bd_Obg/CgtA.
IPR015349. GTP-bd_prot_GTP1/OBG_C.
IPR006074. GTP1-OBG_CS.
IPR006169. GTP1_OBG_dom.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR11702:SF3. PTHR11702:SF3. 1 hit.
Pfami PF09269. DUF1967. 1 hit.
PF01018. GTP1_OBG. 1 hit.
PF01926. MMR_HSR1. 1 hit.
[Graphical view ]
PIRSFi PIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
PRINTSi PR00326. GTP1OBG.
SUPFAMi SSF102741. SSF102741. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF82051. SSF82051. 1 hit.
TIGRFAMsi TIGR02729. Obg_CgtA. 1 hit.
TIGR03595. Obg_CgtA_exten. 1 hit.
PROSITEi PS51710. G_OBG. 1 hit.
PS00905. GTP1_OBG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation."
    Okamoto S., Itoh M., Ochi K.
    J. Bacteriol. 179:170-179(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor."
    Okamoto S., Ochi K.
    Mol. Microbiol. 30:107-119(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PRO-168; GLY-171; LYS-172; SER-173; LEU-281 AND ASP-285, INDUCTION, SUBCELLULAR LOCATION, OVEREXPRESSION, DISRUPTION PHENOTYPE.
  4. "Obg/CtgA, a signaling protein that controls replication, translation, and morphological development?"
    Michel B.
    Dev. Cell 8:300-301(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiOBG_STRCO
AccessioniPrimary (citable) accession number: P95722
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3