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P95722

- OBG_STRCO

UniProt

P95722 - OBG_STRCO

Protein

GTPase Obg

Gene

obg

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Plays an unknown essential role and a regulatory role in sporulation. Overexpression suppresses sporulation although cell growth rate was not reduced. Impaired differentiation was eliminated by addition of decoyinine, an inhibitor of GMP synthesis. Overexpression has no effect on undecylprodigiosin production, but decreases actinorhodin production.
    An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in those bacteria that undergo differentiation, in morphogenesis control.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi166 – 1738GTPUniRule annotation
    Nucleotide bindingi212 – 2165GTPUniRule annotation
    Nucleotide bindingi282 – 2854GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase ObgUniRule annotation
    Alternative name(s):
    GTP-binding protein ObgUniRule annotation
    Gene namesi
    Name:obgUniRule annotation
    Ordered Locus Names:SCO2595
    ORF Names:SCC88.06c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation. Cell membrane 1 Publication; Peripheral membrane protein 1 Publication
    Note: No longer associated with the cell membrane after salt-washing.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Essential for growth, it cannot be disrupted.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi168 – 1681P → V: Overexpression suppresses sporulation more than the wild-type gene. 1 Publication
    Mutagenesisi171 – 1711G → A: Overexpression enhances aerial mycelium formation, also enhances actinorhodin production. 1 Publication
    Mutagenesisi172 – 1721K → N: No phenotype upon overexpression. 1 Publication
    Mutagenesisi173 – 1731S → N: No phenotype upon overexpression. 1 Publication
    Mutagenesisi281 – 2811L → I: No phenotype upon overexpression. 1 Publication
    Mutagenesisi285 – 2851D → A: No phenotype upon overexpression. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478GTPase ObgPRO_0000386323Add
    BLAST

    Expressioni

    Inductioni

    Expressed during vegetative growth it disappears during stationary phase and sporulation (at protein level).1 Publication

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi100226.SCO2595.

    Structurei

    3D structure databases

    ProteinModelPortaliP95722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini160 – 330171OBG-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0536.
    HOGENOMiHOG000019083.
    KOiK03979.
    OMAiNGGHGCA.
    OrthoDBiEOG6H1Q1M.
    PhylomeDBiP95722.

    Family and domain databases

    Gene3Di2.70.210.12. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_01454. GTPase_Obg.
    InterProiIPR014100. GTP-bd_Obg/CgtA.
    IPR015349. GTP-bd_prot_GTP1/OBG_C.
    IPR006074. GTP1-OBG_CS.
    IPR006169. GTP1_OBG_dom.
    IPR006073. GTP_binding_domain.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR11702:SF3. PTHR11702:SF3. 1 hit.
    PfamiPF09269. DUF1967. 1 hit.
    PF01018. GTP1_OBG. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
    PRINTSiPR00326. GTP1OBG.
    SUPFAMiSSF102741. SSF102741. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF82051. SSF82051. 1 hit.
    TIGRFAMsiTIGR02729. Obg_CgtA. 1 hit.
    TIGR03595. Obg_CgtA_exten. 1 hit.
    PROSITEiPS51710. G_OBG. 1 hit.
    PS00905. GTP1_OBG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P95722-1 [UniParc]FASTAAdd to Basket

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    MTTFVDRVEL HVAAGNGGHG CASVHREKFK PLGGPDGGNG GRGGDVILTV    50
    DQSVTTLLDY HHSPHRKATN GKPGEGGNRS GKDGQDLVLP VPDGTVVLDG 100
    AGNVLADLVG HGTSYVAAQG GRGGLGNAAL ASARRKAPGF ALLGEPGDLQ 150
    DIHLELKTVA DVALVGYPSA GKSSLISVLS AAKPKIADYP FTTLVPNLGV 200
    VTAGETVYTV ADVPGLIPGA SQGKGLGLEF LRHVERCSVL VHVLDTATLE 250
    SERDPLSDLD VIETELREYG GLDNRPRIVV LNKIDVPDGK DLAEMVRPDL 300
    EARGYRVFEV SAVAHMGLRE LSFALAELVA TARAARPKEE ATRIVIRPKA 350
    VDDAGFTVTR EEDGLFRVRG EKPERWVRQT DFNNDEAVGY LSDRLNRLGV 400
    EDKLMKAGAR NGDGVAIGPE DNAVVFDWEP SVTAGAEMLG RRGEDHRFEA 450
    PRPAAQRRRD RDAERDEAQQ EFDGFEPF 478
    Length:478
    Mass (Da):51,073
    Last modified:May 1, 1997 - v1
    Checksum:iACAFBCDCF4C9E53B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87915 Genomic DNA. Translation: BAA13498.1.
    AL939113 Genomic DNA. Translation: CAB75376.1.
    PIRiT42036.
    RefSeqiNP_626832.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB75376; CAB75376; CAB75376.
    GeneIDi1098029.
    KEGGisco:SCO2595.
    PATRICi23734876. VBIStrCoe124346_2641.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D87915 Genomic DNA. Translation: BAA13498.1 .
    AL939113 Genomic DNA. Translation: CAB75376.1 .
    PIRi T42036.
    RefSeqi NP_626832.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali P95722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO2595.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB75376 ; CAB75376 ; CAB75376 .
    GeneIDi 1098029.
    KEGGi sco:SCO2595.
    PATRICi 23734876. VBIStrCoe124346_2641.

    Phylogenomic databases

    eggNOGi COG0536.
    HOGENOMi HOG000019083.
    KOi K03979.
    OMAi NGGHGCA.
    OrthoDBi EOG6H1Q1M.
    PhylomeDBi P95722.

    Family and domain databases

    Gene3Di 2.70.210.12. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_01454. GTPase_Obg.
    InterProi IPR014100. GTP-bd_Obg/CgtA.
    IPR015349. GTP-bd_prot_GTP1/OBG_C.
    IPR006074. GTP1-OBG_CS.
    IPR006169. GTP1_OBG_dom.
    IPR006073. GTP_binding_domain.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR11702:SF3. PTHR11702:SF3. 1 hit.
    Pfami PF09269. DUF1967. 1 hit.
    PF01018. GTP1_OBG. 1 hit.
    PF01926. MMR_HSR1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002401. GTP_bd_Obg/CgtA. 1 hit.
    PRINTSi PR00326. GTP1OBG.
    SUPFAMi SSF102741. SSF102741. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF82051. SSF82051. 1 hit.
    TIGRFAMsi TIGR02729. Obg_CgtA. 1 hit.
    TIGR03595. Obg_CgtA_exten. 1 hit.
    PROSITEi PS51710. G_OBG. 1 hit.
    PS00905. GTP1_OBG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the obg gene of Streptomyces griseus in relation to the onset of morphological differentiation."
      Okamoto S., Itoh M., Ochi K.
      J. Bacteriol. 179:170-179(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "An essential GTP-binding protein functions as a regulator for differentiation in Streptomyces coelicolor."
      Okamoto S., Ochi K.
      Mol. Microbiol. 30:107-119(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF PRO-168; GLY-171; LYS-172; SER-173; LEU-281 AND ASP-285, INDUCTION, SUBCELLULAR LOCATION, OVEREXPRESSION, DISRUPTION PHENOTYPE.
    4. "Obg/CtgA, a signaling protein that controls replication, translation, and morphological development?"
      Michel B.
      Dev. Cell 8:300-301(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiOBG_STRCO
    AccessioniPrimary (citable) accession number: P95722
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 13, 2009
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3